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Literature summary extracted from

  • Hong, S.K.; Kim, K.H.; Park, J.K.; Jeong, K.W.; Kim, Y.; Kim, E.E.
    New design platform for malonyl-CoA-acyl carrier protein transacylase (2010), FEBS Lett., 584, 1240-1244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 expressed in Escherichia coli Streptococcus pneumoniae
2.3.1.39 expressed in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.39 crystal structures of MCAT from Staphylococcus aureus and Streptococcus pneumoniae are determined at 1.46 and 2.1 A resolution, respectively Streptococcus pneumoniae
2.3.1.39 crystal structures of MCAT from Staphylococcus aureus and Streptococcus pneumoniae are determined at 1.46 and 2.1 A resolution, respectively. In the SaMCAT structure, the N-terminal expression peptide of a neighboring molecule running in the opposite direction of malonyl-CoA makes extensive interactions with the highly conserved Gly-Gln-Gly-Ser-Gln stretch, suggesting a new design platform Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.39 F198S mutant shows a reduction in activity Streptococcus pneumoniae
2.3.1.39 H199A mutant shows a drastic reduction in activity Streptococcus pneumoniae
2.3.1.39 L89A mutant shows almost the same activity as wild-type Streptococcus pneumoniae
2.3.1.39 N158A mutant shows almost the same activity as wild-type Streptococcus pneumoniae
2.3.1.39 R115A mutant shows a reduction in activity Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Staphylococcus aureus Q99UN8
-
-
2.3.1.39 Streptococcus pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein alpha-ketoglutarate dehydrogenase (KDH)-coupled assay system is used: KDH-dependent consumption of coenzyme A (CoA) generated by MCAT is accompanied by a reduction of nicotinamide adenine dinucleotide (NAD+) to NADH Streptococcus pneumoniae CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein alpha-ketoglutarate dehydrogenase (KDH)-coupled assay system is used: KDH-dependent consumption of coenzyme A (CoA) generated by MCAT is accompanied by a reduction of nicotinamide adenine dinucleotide (NAD+) to NADH Staphylococcus aureus CoA + malonyl-[acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 malonyl-CoA-acyl carrier protein transacylase
-
 Streptococcus pneumoniae
2.3.1.39 malonyl-CoA-acyl carrier protein transacylase
-
 Staphylococcus aureus
2.3.1.39 SaMCAT
-
 Staphylococcus aureus
2.3.1.39 SpnMCAT
-
 Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 6.8
-
 assay at Streptococcus pneumoniae
2.3.1.39 6.8
-
 assay at Staphylococcus aureus