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Literature summary extracted from

  • Lamble, H.J.; Theodossis, A.; Milburn, C.C.; Taylor, G.L.;, Bull, S.D.; Hough, D.W.; Danson, M.J.
    Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus (2005), FEBS Lett., 579, 6865-6869.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.178 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.178 2.8
-
 ATP pH 7.5, 60°C Saccharolobus solfataricus
2.7.1.178 3.6
-
 2-dehydro-3-deoxy-D-gluconate pH 7.5, 60°C Saccharolobus solfataricus
2.7.1.178 8.1
-
 2-dehydro-3-deoxy-D-galactonate pH 7.5, 60°C Saccharolobus solfataricus
4.1.2.55 0.1
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus
4.1.2.55 0.17
-
 2-dehydro-3-deoxy-D-galactonate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus
4.1.2.55 9.9
-
 2-dehydro-3-deoxy-D-galactonate 60°C, pH not specified in the publication Saccharolobus solfataricus
4.1.2.55 25.7
-
 2-dehydro-3-deoxy-D-gluconate 60°C, pH not specified in the publication Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.178 34875
-
 x * 34875, calculated from sequence Saccharolobus solfataricus
2.7.1.178 37513
-
 x * 37513, mass spectrometric analysis Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus
-
 ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus
-
 ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus
-
 pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate 6-phosphate Saccharolobus solfataricus
-
 pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus comparison of the catalytic efficiency (kcat/KM) for 2-dehydro-3-deoxy-D-gluconate (1.1 mM/s) and 2-dehydro-3-deoxy-D-gluconate 6-phosphate (643 mM/s) suggests that cleavage of the phosphorylated substrate would be significantly favoured in a situation where both compounds are present. If the 2-dehydro-3-deoxy-D-gluconate kinase is present and active then central metabolism will occur via the part-phosphorylative route.The Km of the kinase for ATP of 2.8 mM is high compared to the likely intracellular concentration, and it is possible that the non-phosphorylative pathway would be favoured under starvation conditions, which could produce pyruvate to allow the citric acid cycle to function without ATP input. regardless of whether Sulfolobus solfataricus uses the part-phosphorylative or the non-phosphorylative Entner-Doudoroff pathway, then the same enzymes can be used for the metabolism of glucose and its C4 epimer galactose pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate 6-phosphate Saccharolobus solfataricus
-
 pyruvate + D-glyceraldehyde 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.178 Saccharolobus solfataricus Q97U29
-
-
4.1.2.55 Saccharolobus solfataricus O54288
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.178
-
 Saccharolobus solfataricus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-galactonate = ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate (2) Saccharolobus solfataricus
a
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate = ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate (1) Saccharolobus solfataricus
a
4.1.2.55 2-dehydro-3-deoxy-6-phospho-D-galactonate = pyruvate + D-glyceraldehyde 3-phosphate (2) Saccharolobus solfataricus
a
4.1.2.55 2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate (1) Saccharolobus solfataricus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-galactonate
-
 Saccharolobus solfataricus ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-galactonate 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate are phosphorylated with similar catalytic efficiency Saccharolobus solfataricus ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate
-
 Saccharolobus solfataricus ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
2.7.1.178 ATP + 2-dehydro-3-deoxy-D-gluconate 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate are phosphorylated with similar catalytic efficiency Saccharolobus solfataricus ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate
-
 Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate catalytic efficiency (kcat/KM) is 295fold lower compared to 2-dehydro-3-deoxy-D-galactonate 6-phosphate Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
 Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-galactonate 6-phosphate catalytic efficiency (kcat/KM) is 295fold higher compared to 2-dehydro-3-deoxy-D-galactonate Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate comparison of the catalytic efficiency (kcat/KM) for 2-dehydro-3-deoxy-D-gluconate (1.1 mM/s) and 2-dehydro-3-deoxy-D-gluconate 6-phosphate (643 mM/s) suggests that cleavage of the phosphorylated substrate would be significantly favoured in a situation where both compounds are present. If the 2-dehydro-3-deoxy-D-gluconate kinase is present and active then central metabolism will occur via the part-phosphorylative route.The Km of the kinase for ATP of 2.8 mM is high compared to the likely intracellular concentration, and it is possible that the non-phosphorylative pathway would be favoured under starvation conditions, which could produce pyruvate to allow the citric acid cycle to function without ATP input. regardless of whether Sulfolobus solfataricus uses the part-phosphorylative or the non-phosphorylative Entner-Doudoroff pathway, then the same enzymes can be used for the metabolism of glucose and its C4 epimer galactose Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate catalytic efficiency (kcat/KM) is 580fold lower compared to 2-dehydro-3-deoxy-D-gluconate 6-phosphate Saccharolobus solfataricus pyruvate + D-glyceraldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
 Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-gluconate 6-phosphate catalytic efficiency (kcat/KM) is 580fold higher compared to 2-dehydro-3-deoxy-D-gluconate Saccharolobus solfataricus pyruvate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.178 ? x * 34875, calculated from sequence Saccharolobus solfataricus
2.7.1.178 ? x * 37513, mass spectrometric analysis Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.178 KDG kinase
-
 Saccharolobus solfataricus
4.1.2.55 KDG aldolase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.178 60
-
 assay at Saccharolobus solfataricus
4.1.2.55 60
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.178 3.8
-
 ATP pH 7.5, 60°C Saccharolobus solfataricus
2.7.1.178 5
-
 2-dehydro-3-deoxy-D-gluconate pH 7.5, 60°C Saccharolobus solfataricus
2.7.1.178 5.4
-
 2-dehydro-3-deoxy-D-galactonate pH 7.5, 60°C Saccharolobus solfataricus
4.1.2.55 6.8
-
 2-dehydro-3-deoxy-D-galactonate 60°C, pH not specified in the publication Saccharolobus solfataricus
4.1.2.55 28.2
-
 2-dehydro-3-deoxy-D-gluconate 60°C, pH not specified in the publication Saccharolobus solfataricus
4.1.2.55 34.8
-
 2-dehydro-3-deoxy-D-galactonate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus
4.1.2.55 61.9
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.178 7.5
-
 assay at Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
4.1.2.55 physiological function comparison of the catalytic efficiency (kcat/KM) for 2-dehydro-3-deoxy-D-gluconate (1.1 mM/s) and 2-dehydro-3-deoxy-D-gluconate 6-phosphate (643 mM/s) suggests that cleavage of the phosphorylated substrate would be significantly favoured in a situation where both compounds are present. If the 2-dehydro-3-deoxy-D-gluconate kinase is present and active then central metabolism will occur via the part-phosphorylative route.The Km of the kinase for ATP of 2.8 mM is high compared to the likely intracellular concentration, and it is possible that the non-phosphorylative pathway would be favoured under starvation conditions, which could produce pyruvate to allow the citric acid cycle to function without ATP input. regardless of whether Sulfolobus solfataricus uses the part-phosphorylative or the non-phosphorylative Entner-Doudoroff pathway, then the same enzymes can be used for the metabolism of glucose and its C4 epimer galactose Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.178 0.7
-
 2-dehydro-3-deoxy-D-galactonate pH 7.5, 60°C Saccharolobus solfataricus
2.7.1.178 1.4
-
 ATP pH 7.5, 60°C Saccharolobus solfataricus
2.7.1.178 1.4
-
 2-dehydro-3-deoxy-D-gluconate pH 7.5, 60°C Saccharolobus solfataricus
4.1.2.55 0.7
-
 2-dehydro-3-deoxy-D-galactonate 60°C, pH not specified in the publication Saccharolobus solfataricus
4.1.2.55 1.1
-
 2-dehydro-3-deoxy-D-gluconate 60°C, pH not specified in the publication Saccharolobus solfataricus
4.1.2.55 207
-
 2-dehydro-3-deoxy-D-galactonate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus
4.1.2.55 643
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate 60°C, pH not specified in the publication Saccharolobus solfataricus