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Literature summary extracted from

  • Janowitz, T.; Ajonina, I.; Perbandt, M.; Woltersdorf, C.; Hertel, P.; Liebau, E.; Gigengack, U.
    The 3-ureidopropionase of Caenorhabditis elegans, an enzyme involved in pyrimidine degradation (2010), FEBS J., 277, 4100-4109.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.6 hexahistidine fusion protein expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.6 0.147
-
 3-ureidopropionic acid pH 7.5, 30°C Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.6 43200
-
 12 * 43200 Caenorhabditis elegans
3.5.1.6 472000
-
 gel filtration, blue native PAGE Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.6 Caenorhabditis elegans Q19437
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.6 immobilized metal ion affinity chromatography (Ni2+) Caenorhabditis elegans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.6 striated muscle cell
-
 Caenorhabditis elegans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.6 1.1
-
 pH 7.5, 30°C Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.6 2-methyl-3-ureidopropionic acid + H2O 82% activity in comparison to 3-ureidopropionic acid Caenorhabditis elegans 3-amino-2-methylpropanoic acid + NH3 + CO2
-
 ?
3.5.1.6 3-ureidopropionic acid + H2O
-
 Caenorhabditis elegans beta-alanine + CO2 + NH3
-
 ?
3.5.1.6 4-amino-4-oxobutyric acid + H2O 3% activity in comparison to 3-ureidopropionic acid Caenorhabditis elegans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.6 dodecamer 12 * 43200 Caenorhabditis elegans

Synonyms

EC Number Synonyms Comment Organism
3.5.1.6 3-ureidopropionase
-
 Caenorhabditis elegans
3.5.1.6 beta-alanine synthase
-
 Caenorhabditis elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.6 40
-
 unstable, 65 s half-life Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.6 7 8 broad optimum Caenorhabditis elegans