BRENDA - Enzyme Database show

Molecular and biochemical characterization of the recombinant amidase from hyperthermophilic archaeon Sulfolobus solfataricus

dAbusco, A.S.; Ammendola, S.; Scandurra, R.; Politi, L.; Extremophiles 5, 183-192 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.4
overexpression in Escherichia coli as a fusion protein with an N-terminus six-histidine-residue affinity tag
Saccharolobus solfataricus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.3
-
2-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
2-toluamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
4-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
Benzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
cyclomaltohexaose
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
nicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.4
-
2-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.8
-
Isonicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.9
-
3-indolacetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1
-
Isobutyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.2
-
butyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.2
-
methacrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.3
-
Pentanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.3
-
Propionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.5
-
4-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.5
-
picolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3
-
Acrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3
-
lactamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.6
-
acetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
4
-
3-phenylpropionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
5
-
cyclohexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
5.8
-
hexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
6.7
-
L-prolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.4
Co2+
1 mM, about 20% activation
Saccharolobus solfataricus
3.5.1.4
Fe3+
1 mM, about 20% activation
Saccharolobus solfataricus
3.5.1.4
additional information
no effect: Cu2+, Mn2+, and Ca2+
Saccharolobus solfataricus
3.5.1.4
V2+
1 mM, about 20% activation
Saccharolobus solfataricus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.4
55000
-
x * 55000, SDS-PAGE
Saccharolobus solfataricus
3.5.1.4
55784
-
x * 55784, calculated from sequence
Saccharolobus solfataricus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.4
Saccharolobus solfataricus
P95896
-
-
3.5.1.4
Saccharolobus solfataricus MT4
P95896
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.4
-
Saccharolobus solfataricus
Storage Stability
EC Number
Storage Stability
Organism
3.5.1.4
the enzyme may be stored at room temperature in the presence of 0.04% NaN3 for 1 year without any loss of activity
Saccharolobus solfataricus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
(S)-2-phenylpropionamide + H2O
no activity with (R)-2-phenylpropionamide
719432
Saccharolobus solfataricus
(S)-2-phenylpropionate + NH3
-
-
-
?
3.5.1.4
2-aminobenzamide + H2O
-
719432
Saccharolobus solfataricus
2-aminobenzoic acid + NH3
-
-
-
?
3.5.1.4
2-hydroxybenzamide + H2O
-
719432
Saccharolobus solfataricus
2-hydroxybenzoic acid + NH3
-
-
-
?
3.5.1.4
2-thiophenecarboxamide + H2O
-
719432
Saccharolobus solfataricus
2-thiophenecarboxylate + NH3
-
-
-
?
3.5.1.4
2-toluamide + H2O
-
719432
Saccharolobus solfataricus
2-toluic acid + NH3
-
-
-
?
3.5.1.4
3-indolacetamide + H2O
-
719432
Saccharolobus solfataricus
3-indolacetate + NH3
-
-
-
?
3.5.1.4
3-phenylpropionamide + H2O
-
719432
Saccharolobus solfataricus
3-phenylpropionate + NH3
-
-
-
?
3.5.1.4
3-toluamide + H2O
-
719432
Saccharolobus solfataricus
3-toluic acid + NH3
-
-
-
?
3.5.1.4
4-aminobenzamide + H2O
-
719432
Saccharolobus solfataricus
4-aminobenzoic acid + NH3
-
-
-
?
3.5.1.4
4-hydroxybenzamide + H2O
-
719432
Saccharolobus solfataricus
4-hydroxybenzoic acid + NH3
-
-
-
?
3.5.1.4
4-methylbenzamide + H2O
best aromatic substrate
719432
Saccharolobus solfataricus
4-methylbenzoic acid + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
719432
Saccharolobus solfataricus
acetate + NH3
-
-
-
?
3.5.1.4
acrylamide + H2O
-
719432
Saccharolobus solfataricus
acrylate + NH3
-
-
-
?
3.5.1.4
anthranilamide + H2O
-
719432
Saccharolobus solfataricus
anthranilate + NH3
-
-
-
?
3.5.1.4
benzamide + H2O
-
719432
Saccharolobus solfataricus
benzoate + NH3
-
-
-
?
3.5.1.4
butyramide + H2O
-
719432
Saccharolobus solfataricus
butyrate + NH3
-
-
-
?
3.5.1.4
cyclohexanamide + H2O
-
719432
Saccharolobus solfataricus
cyclohexanoate + NH3
-
-
-
?
3.5.1.4
hexanamide + H2O
-
719432
Saccharolobus solfataricus
hexanoate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
best aliphatic substrate
719432
Saccharolobus solfataricus
isobutyrate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
best aliphatic substrate
719432
Saccharolobus solfataricus MT4
isobutyrate + NH3
-
-
-
?
3.5.1.4
isonicotinamide + H2O
-
719432
Saccharolobus solfataricus
isonicotinic acid + NH3
-
-
-
?
3.5.1.4
L-asparagine + H2O
-
719432
Saccharolobus solfataricus
L-aspartate + NH3
-
-
-
?
3.5.1.4
L-asparagine + H2O
-
719432
Saccharolobus solfataricus MT4
L-aspartate + NH3
-
-
-
?
3.5.1.4
L-glutamine + H2O
-
719432
Saccharolobus solfataricus
L-glutamate + NH3
-
-
-
?
3.5.1.4
L-glutamine + H2O
-
719432
Saccharolobus solfataricus MT4
L-glutamate + NH3
-
-
-
?
3.5.1.4
L-prolinamide + H2O
-
719432
Saccharolobus solfataricus
L-proline + NH3
-
-
-
?
3.5.1.4
lactamide + H2O
-
719432
Saccharolobus solfataricus
lactate + NH3
-
-
-
?
3.5.1.4
methacrylamide + H2O
-
719432
Saccharolobus solfataricus
methacrylate + NH3
-
-
-
?
3.5.1.4
additional information
the enzyme transfers the acyl group also to hydroxylamine and hydrazine
719432
Saccharolobus solfataricus
?
-
-
-
-
3.5.1.4
additional information
the enzyme transfers the acyl group also to hydroxylamine and hydrazine
719432
Saccharolobus solfataricus MT4
?
-
-
-
-
3.5.1.4
nicotinamide + H2O
-
719432
Saccharolobus solfataricus
nicotinic acid + NH3
-
-
-
?
3.5.1.4
pentanamide + H2O
-
719432
Saccharolobus solfataricus
pentanoate + NH3
-
-
-
?
3.5.1.4
pentanamide + H2O
-
719432
Saccharolobus solfataricus MT4
pentanoate + NH3
-
-
-
?
3.5.1.4
picolinamide + H2O
-
719432
Saccharolobus solfataricus
picolinate + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
719432
Saccharolobus solfataricus
propionate + NH3
-
-
-
?
3.5.1.4
pyrazinamide + H2O
-
719432
Saccharolobus solfataricus
pyrazinoic acid + NH3
-
-
-
?
3.5.1.4
salicylamide + H2O
-
719432
Saccharolobus solfataricus
salicylate + NH3
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.4
?
x * 55000, SDS-PAGE; x * 55784, calculated from sequence
Saccharolobus solfataricus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.4
70
-
assay at
Saccharolobus solfataricus
3.5.1.4
95
-
-
Saccharolobus solfataricus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.4
75
95
75°C: about 50% of maximal activity, 95°C: maximal activity, 100°C: less than 10% of activity
Saccharolobus solfataricus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.4
70
-
activity is constant for more than 72 h
Saccharolobus solfataricus
3.5.1.4
80
-
half-life: 25 h
Saccharolobus solfataricus
3.5.1.4
90
-
half-life: 0.5 h
Saccharolobus solfataricus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.3
-
2-toluamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.8
-
4-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.9
-
2-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.1
-
4-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.3
-
2-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.6
-
acetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.6
-
Isonicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.6
-
nicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.8
-
3-indolacetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.8
-
picolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.9
-
methacrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.1
-
L-prolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.2
-
Propionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
5.3
-
hexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
6.4
-
Benzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
7.1
-
3-phenylpropionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
7.3
-
cyclomaltohexaose
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
8.3
-
Acrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
9.9
-
butyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
10.7
-
lactamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
16.3
-
cyclohexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
20.5
-
Pentanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
21.9
-
Isobutyramide
pH 7.5, 70°C
Saccharolobus solfataricus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.4
7.5
-
-
Saccharolobus solfataricus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.4
4
9.5
pH 4: about 50% of maximal activity, pH 9.5: about 50% of maximal activity
Saccharolobus solfataricus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.1.4
Saccharolobus solfataricus
protein without His-tag tail calculated from amino acid composition
-
5.94
3.5.1.4
Saccharolobus solfataricus
protein with His-tag tail calculated from amino acid composition
-
6.05
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.4
overexpression in Escherichia coli as a fusion protein with an N-terminus six-histidine-residue affinity tag
Saccharolobus solfataricus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.3
-
2-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
2-toluamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
4-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
Benzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
cyclomaltohexaose
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
nicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.4
-
2-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.8
-
Isonicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.9
-
3-indolacetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1
-
Isobutyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.2
-
butyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.2
-
methacrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.3
-
Pentanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.3
-
Propionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.5
-
4-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.5
-
picolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3
-
Acrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3
-
lactamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.6
-
acetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
4
-
3-phenylpropionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
5
-
cyclohexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
5.8
-
hexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
6.7
-
L-prolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.4
Co2+
1 mM, about 20% activation
Saccharolobus solfataricus
3.5.1.4
Fe3+
1 mM, about 20% activation
Saccharolobus solfataricus
3.5.1.4
additional information
no effect: Cu2+, Mn2+, and Ca2+
Saccharolobus solfataricus
3.5.1.4
V2+
1 mM, about 20% activation
Saccharolobus solfataricus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.4
55000
-
x * 55000, SDS-PAGE
Saccharolobus solfataricus
3.5.1.4
55784
-
x * 55784, calculated from sequence
Saccharolobus solfataricus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.4
-
Saccharolobus solfataricus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.1.4
the enzyme may be stored at room temperature in the presence of 0.04% NaN3 for 1 year without any loss of activity
Saccharolobus solfataricus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
(S)-2-phenylpropionamide + H2O
no activity with (R)-2-phenylpropionamide
719432
Saccharolobus solfataricus
(S)-2-phenylpropionate + NH3
-
-
-
?
3.5.1.4
2-aminobenzamide + H2O
-
719432
Saccharolobus solfataricus
2-aminobenzoic acid + NH3
-
-
-
?
3.5.1.4
2-hydroxybenzamide + H2O
-
719432
Saccharolobus solfataricus
2-hydroxybenzoic acid + NH3
-
-
-
?
3.5.1.4
2-thiophenecarboxamide + H2O
-
719432
Saccharolobus solfataricus
2-thiophenecarboxylate + NH3
-
-
-
?
3.5.1.4
2-toluamide + H2O
-
719432
Saccharolobus solfataricus
2-toluic acid + NH3
-
-
-
?
3.5.1.4
3-indolacetamide + H2O
-
719432
Saccharolobus solfataricus
3-indolacetate + NH3
-
-
-
?
3.5.1.4
3-phenylpropionamide + H2O
-
719432
Saccharolobus solfataricus
3-phenylpropionate + NH3
-
-
-
?
3.5.1.4
3-toluamide + H2O
-
719432
Saccharolobus solfataricus
3-toluic acid + NH3
-
-
-
?
3.5.1.4
4-aminobenzamide + H2O
-
719432
Saccharolobus solfataricus
4-aminobenzoic acid + NH3
-
-
-
?
3.5.1.4
4-hydroxybenzamide + H2O
-
719432
Saccharolobus solfataricus
4-hydroxybenzoic acid + NH3
-
-
-
?
3.5.1.4
4-methylbenzamide + H2O
best aromatic substrate
719432
Saccharolobus solfataricus
4-methylbenzoic acid + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
719432
Saccharolobus solfataricus
acetate + NH3
-
-
-
?
3.5.1.4
acrylamide + H2O
-
719432
Saccharolobus solfataricus
acrylate + NH3
-
-
-
?
3.5.1.4
anthranilamide + H2O
-
719432
Saccharolobus solfataricus
anthranilate + NH3
-
-
-
?
3.5.1.4
benzamide + H2O
-
719432
Saccharolobus solfataricus
benzoate + NH3
-
-
-
?
3.5.1.4
butyramide + H2O
-
719432
Saccharolobus solfataricus
butyrate + NH3
-
-
-
?
3.5.1.4
cyclohexanamide + H2O
-
719432
Saccharolobus solfataricus
cyclohexanoate + NH3
-
-
-
?
3.5.1.4
hexanamide + H2O
-
719432
Saccharolobus solfataricus
hexanoate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
best aliphatic substrate
719432
Saccharolobus solfataricus
isobutyrate + NH3
-
-
-
?
3.5.1.4
isobutyramide + H2O
best aliphatic substrate
719432
Saccharolobus solfataricus MT4
isobutyrate + NH3
-
-
-
?
3.5.1.4
isonicotinamide + H2O
-
719432
Saccharolobus solfataricus
isonicotinic acid + NH3
-
-
-
?
3.5.1.4
L-asparagine + H2O
-
719432
Saccharolobus solfataricus
L-aspartate + NH3
-
-
-
?
3.5.1.4
L-asparagine + H2O
-
719432
Saccharolobus solfataricus MT4
L-aspartate + NH3
-
-
-
?
3.5.1.4
L-glutamine + H2O
-
719432
Saccharolobus solfataricus
L-glutamate + NH3
-
-
-
?
3.5.1.4
L-glutamine + H2O
-
719432
Saccharolobus solfataricus MT4
L-glutamate + NH3
-
-
-
?
3.5.1.4
L-prolinamide + H2O
-
719432
Saccharolobus solfataricus
L-proline + NH3
-
-
-
?
3.5.1.4
lactamide + H2O
-
719432
Saccharolobus solfataricus
lactate + NH3
-
-
-
?
3.5.1.4
methacrylamide + H2O
-
719432
Saccharolobus solfataricus
methacrylate + NH3
-
-
-
?
3.5.1.4
additional information
the enzyme transfers the acyl group also to hydroxylamine and hydrazine
719432
Saccharolobus solfataricus
?
-
-
-
-
3.5.1.4
additional information
the enzyme transfers the acyl group also to hydroxylamine and hydrazine
719432
Saccharolobus solfataricus MT4
?
-
-
-
-
3.5.1.4
nicotinamide + H2O
-
719432
Saccharolobus solfataricus
nicotinic acid + NH3
-
-
-
?
3.5.1.4
pentanamide + H2O
-
719432
Saccharolobus solfataricus
pentanoate + NH3
-
-
-
?
3.5.1.4
pentanamide + H2O
-
719432
Saccharolobus solfataricus MT4
pentanoate + NH3
-
-
-
?
3.5.1.4
picolinamide + H2O
-
719432
Saccharolobus solfataricus
picolinate + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
719432
Saccharolobus solfataricus
propionate + NH3
-
-
-
?
3.5.1.4
pyrazinamide + H2O
-
719432
Saccharolobus solfataricus
pyrazinoic acid + NH3
-
-
-
?
3.5.1.4
salicylamide + H2O
-
719432
Saccharolobus solfataricus
salicylate + NH3
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.4
?
x * 55000, SDS-PAGE; x * 55784, calculated from sequence
Saccharolobus solfataricus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.4
70
-
assay at
Saccharolobus solfataricus
3.5.1.4
95
-
-
Saccharolobus solfataricus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.4
75
95
75°C: about 50% of maximal activity, 95°C: maximal activity, 100°C: less than 10% of activity
Saccharolobus solfataricus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.4
70
-
activity is constant for more than 72 h
Saccharolobus solfataricus
3.5.1.4
80
-
half-life: 25 h
Saccharolobus solfataricus
3.5.1.4
90
-
half-life: 0.5 h
Saccharolobus solfataricus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.3
-
2-toluamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.8
-
4-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.9
-
2-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.1
-
4-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.3
-
2-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.6
-
acetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.6
-
Isonicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.6
-
nicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.8
-
3-indolacetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.8
-
picolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.9
-
methacrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.1
-
L-prolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.2
-
Propionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
5.3
-
hexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
6.4
-
Benzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
7.1
-
3-phenylpropionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
7.3
-
cyclomaltohexaose
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
8.3
-
Acrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
9.9
-
butyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
10.7
-
lactamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
16.3
-
cyclohexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
20.5
-
Pentanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
21.9
-
Isobutyramide
pH 7.5, 70°C
Saccharolobus solfataricus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.4
7.5
-
-
Saccharolobus solfataricus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.4
4
9.5
pH 4: about 50% of maximal activity, pH 9.5: about 50% of maximal activity
Saccharolobus solfataricus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.5.1.4
Saccharolobus solfataricus
protein without His-tag tail calculated from amino acid composition
-
5.94
3.5.1.4
Saccharolobus solfataricus
protein with His-tag tail calculated from amino acid composition
-
6.05
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.27
-
4-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
2-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.44
-
acetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.46
-
L-prolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.72
-
picolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.73
-
4-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.75
-
2-toluamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.91
-
hexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.8
-
3-phenylpropionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2
-
3-indolacetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2
-
Isonicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.4
-
methacrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.46
-
Propionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.8
-
Acrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.3
-
2-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.3
-
cyclohexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.57
-
lactamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
5.3
-
nicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
8.25
-
butyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
15.7
-
Pentanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
21.3
-
Benzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
21.9
-
Isobutyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
24.3
-
cyclomaltohexaose
pH 7.5, 70°C
Saccharolobus solfataricus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.27
-
4-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.3
-
2-Aminobenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.44
-
acetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.46
-
L-prolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.72
-
picolinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.73
-
4-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.75
-
2-toluamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
0.91
-
hexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
1.8
-
3-phenylpropionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2
-
3-indolacetamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2
-
Isonicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.4
-
methacrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.46
-
Propionamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
2.8
-
Acrylamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.3
-
2-hydroxybenzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.3
-
cyclohexanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
3.57
-
lactamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
5.3
-
nicotinamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
8.25
-
butyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
15.7
-
Pentanamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
21.3
-
Benzamide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
21.9
-
Isobutyramide
pH 7.5, 70°C
Saccharolobus solfataricus
3.5.1.4
24.3
-
cyclomaltohexaose
pH 7.5, 70°C
Saccharolobus solfataricus