Any feedback?
Literature summary extracted from
- Cyclodextrin glycosyltransferase: a key enzyme in the assimilation of starch by the halophilic archaeon Haloferax mediterranei (2012), Extremophiles, 16, 147-159.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.19 | NaCl | enzyme shows its maximum hydrolysis activity in a buffer with 1.5 M NaCl and retains up to 65% of its activity at 0.5 M NaCl | Haloferax mediterranei |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.19 | expression in Escherichia coli | Haloferax mediterranei |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.19 | additional information | - |
additional information | Km value is 17.2 mg/ml for beta-cyclization activity using soluble starch, pH 7.0, 55°C | Haloferax mediterranei |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.1.19 | extracellular | secretion by the Tat pathway | Haloferax mediterranei | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 82000 | - |
x * 82000, SDS-PAGE | Haloferax mediterranei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.19 | Haloferax mediterranei | Q53I75 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.19 | native enzyme | Haloferax mediterranei |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 48 | - |
pH 7.5, 55°C | Haloferax mediterranei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.19 | additional information | cyclization is the predominant activity, followed by hydrolysis and to a lesser extent coupling and disproportionation activities | Haloferax mediterranei | ? | - |
? | |
| 2.4.1.19 | soluble potato starch | - |
Haloferax mediterranei | alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin | ratio of products is alpha-cyclodextrin to beta-cyclodextrin to gamma-cyclodextrin 1:0.6:0.3 | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.19 | ? | x * 82000, SDS-PAGE | Haloferax mediterranei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 55 | - |
presence of 1.5 M NaCl | Haloferax mediterranei |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 35 | - |
50% of maximum activity | Haloferax mediterranei |
| 2.4.1.19 | 60 | - |
50% of maximum activity | Haloferax mediterranei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 7 | - |
presence of 1.5 M NaCl | Haloferax mediterranei |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.19 | 5 | 8 | loss of more than 60% of activity above or below | Haloferax mediterranei |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
