EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.135 | recombinant expression of the truncated tLRAT and its S175R mutant | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.135 | additional information | construction of a truncated enzyme version, tLRAT, that shows enzyme activity, but leads to physiological dysfunction of the protein by causing retinis pigmentosa | Homo sapiens |
2.3.1.135 | S175R | site-directed mutagenesis, the mutation in the truncated LRAT results in an inactive enzyme. The S175R mutation has no effect on the membrane binding properties of tLRAT, the global secondary structure of tLRAT remains almost unchanged with the S175R mutation. The loss of enzymatic activity associated with the S175R mutant is related to loss of an essential nucleophile near the active site, or alternatively to steric obstruction of the active site that impedes substrate binding | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.135 | additional information | - |
additional information | kinetics of truncated tLRAT | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.135 | phosphatidylcholine + retinol-[cellular-retinol-binding-protein] | Homo sapiens | - |
2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.135 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.135 | recombinant truncated tLRAT and its S175R mutant | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.135 | additional information | the enzyme performs formation of all-trans retinyl heptanoate from all-trans-retinol and 1,2-diheptanoyl-sn-glycero-3-phosphocholine, DHPC | Homo sapiens | ? | - |
? | |
2.3.1.135 | phosphatidylcholine + retinol-[cellular-retinol-binding-protein] | - |
Homo sapiens | 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.135 | lecithin retinol acyltransferase | - |
Homo sapiens |
2.3.1.135 | lecithin:retinol acyltransferase | - |
Homo sapiens |
2.3.1.135 | LRAT | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.135 | 20 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.135 | 6 | - |
assay at | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.135 | malfunction | a truncated form of LRAT as well as its S175R mutant lead to retinis pigmentosa, a severe form of retinal dystrophy | Homo sapiens |
2.3.1.135 | additional information | the catalytic triad includes histidine 60, tyrosine 154 and cysteine 161 in the LRAT structure | Homo sapiens |