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Literature summary extracted from

  • Bussieres, S.; Cantin, L.; Salesse, C.
    Lecithin retinol acyltransferase and its S175R mutant have a similar secondary structure content and maximum insertion pressure but different enzyme activities (2011), Exp. Eye Res., 93, 778-781.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.135 recombinant expression of the truncated tLRAT and its S175R mutant Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.135 additional information construction of a truncated enzyme version, tLRAT, that shows enzyme activity, but leads to physiological dysfunction of the protein by causing retinis pigmentosa Homo sapiens
2.3.1.135 S175R site-directed mutagenesis, the mutation in the truncated LRAT results in an inactive enzyme. The S175R mutation has no effect on the membrane binding properties of tLRAT, the global secondary structure of tLRAT remains almost unchanged with the S175R mutation. The loss of enzymatic activity associated with the S175R mutant is related to loss of an essential nucleophile near the active site, or alternatively to steric obstruction of the active site that impedes substrate binding Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.135 additional information
-
additional information kinetics of truncated tLRAT Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.135 phosphatidylcholine + retinol-[cellular-retinol-binding-protein] Homo sapiens
-
2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein]
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.135 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.135 recombinant truncated tLRAT and its S175R mutant Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.135 additional information the enzyme performs formation of all-trans retinyl heptanoate from all-trans-retinol and 1,2-diheptanoyl-sn-glycero-3-phosphocholine, DHPC Homo sapiens ?
-
?
2.3.1.135 phosphatidylcholine + retinol-[cellular-retinol-binding-protein]
-
Homo sapiens 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein]
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.135 lecithin retinol acyltransferase
-
Homo sapiens
2.3.1.135 lecithin:retinol acyltransferase
-
Homo sapiens
2.3.1.135 LRAT
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.135 20
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.135 6
-
assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.135 malfunction a truncated form of LRAT as well as its S175R mutant lead to retinis pigmentosa, a severe form of retinal dystrophy Homo sapiens
2.3.1.135 additional information the catalytic triad includes histidine 60, tyrosine 154 and cysteine 161 in the LRAT structure Homo sapiens