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Literature summary extracted from

  • Castro-Castro, A.; Janke, C.; Montagnac, G.; Paul-Gilloteaux, P.; Chavrier, P.
    ATAT1/MEC-17 acetyltransferase and HDAC6 deacetylase control a balance of acetylation of alpha-tubulin and cortactin and regulate MT1-MMP trafficking and breast tumor cell invasion (2012), Eur. J. Cell Biol., 91, 950-960.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.108 ATAT1, DNA and amino acid sequence determination and analysis, stable functional expression of EGFP-ATAT1 and mutant ATAT1D157N in MDA-MB-231 cells and in HeLa cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.108 D157N site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
2.3.1.108 additional information overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.108 acetyl-CoA + cortactin Homo sapiens ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells CoA + N-acetyl-cortactin
-
 ?
2.3.1.108 acetyl-CoA + [alpha-tubulin]-L-lysine Homo sapiens acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.108 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.108 MDA-MB-231 cell invasive breast cancer cells Homo sapiens
-
2.3.1.108 additional information ATAT1 colocalizes with cortactin at the adherent surface of the cells, association of ATAT1 and cortactin at invadopodia, subcellular localization study, overview Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.108 acetyl-CoA + cortactin
-
 Homo sapiens CoA + N-acetyl-cortactin
-
 ?
2.3.1.108 acetyl-CoA + cortactin ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells Homo sapiens CoA + N-acetyl-cortactin
-
 ?
2.3.1.108 acetyl-CoA + [alpha-tubulin]-L-lysine
-
 Homo sapiens CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
 ?
2.3.1.108 acetyl-CoA + [alpha-tubulin]-L-lysine acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen Homo sapiens CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.108 alpha-tubulin N-acetyltransferase 1
-
 Homo sapiens
2.3.1.108 ATAT1
-
 Homo sapiens
2.3.1.108 ATAT1 acetylase
-
 Homo sapiens
2.3.1.108 ATAT1 tubulin acetyltransferase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.108 acetyl-CoA
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.108 malfunction overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin. Random invasive migration of cells in 3D collagen I is inhibited by 40-50% upon MT1-MMP or ATAT1 knockdown Homo sapiens
2.3.1.108 metabolism a balance of acetylation and deaceylation by ATAT1/HDAC6, histone deacetylase 6, enzymes with opposite activities regulates the migratory and invasive capacities of breast tumor cells Homo sapiens
2.3.1.108 physiological function dynamics and distribution of MT1-MMP-positive endosomes require regulation of acetylation levels, ATAT1 tubulin acetyltransferase binds and regulates cortactin acetylation levels. Acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen. ATAT1 colocalizes with cortactin at the adherent surface of the cells and it is required for 2D migration and invasive migration of MDA-MB-231 cells in collagen matrix Homo sapiens