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Literature summary extracted from

  • Ghanevati, M.; Jaworski, J.
    Engineering and mechanistic studies of the Arabidopsis FAE1 beta-ketoacyl-CoA synthase, FAE1 KCS (2002), Eur. J. Biochem., 269, 3531-3539.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.199 expressed in Saccharomyces cerevisiae strain InvSc1 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.199 C223A the mutant enzyme lacking the acylation site is unable to carry out decarboxylation of malonyl-CoA even when oleic acid-CoA is present Arabidopsis thaliana
2.3.1.199 H391A the mutant enzyme lacking the acylation site is unable to carry out decarboxylation of malonyl-CoA even when oleic acid-CoA is present Arabidopsis thaliana
2.3.1.199 H391K the mutant shows very low condensation activity Arabidopsis thaliana
2.3.1.199 H391Q the mutant shows low condensation activity (25% activity compared to the wild type enzyme) Arabidopsis thaliana
2.3.1.199 N424D the mutant shows low condensation activity Arabidopsis thaliana
2.3.1.199 N424H the mutant enzyme lacking the acylation site is unable to carry out decarboxylation of malonyl-CoA even when oleic acid-CoA is present Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.199 membrane
-
 Arabidopsis thaliana 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.199 43000
-
 x * 43000, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.199 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.199 Ni2+-PDC column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.199 seed
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.199 arachidoyl-CoA + malonyl-CoA 25% activity compared to oleoyl-CoA Arabidopsis thaliana ?
-
 ?
2.3.1.199 additional information the enzyme shows highest activity towards saturated and monounsaturated C16 and C18. In the absence of an acyl-CoA substrate, the enzyme is unable to carry out decarboxylation of malonyl-CoA Arabidopsis thaliana ?
-
 ?
2.3.1.199 additional information the enzyme shows no activity with polyunsaturated linoleic acid and alpha-linolenic acid and little or no activity with acyl-CoAs having 22 carbons or longer in chain length Arabidopsis thaliana ?
-
 ?
2.3.1.199 oleoyl-CoA + malonyl-CoA preferred substrate Arabidopsis thaliana CoA + 3-oxo-eicosenoyl-CoA + 3-oxo-erucoyl-CoA + CO2 3-oxo-eicosenoyl-CoA is the major product ?
2.3.1.199 palmitoleoyl-CoA + malonyl-CoA
-
 Arabidopsis thaliana ?
-
 ?
2.3.1.199 palmitoyl-CoA + malonyl-CoA
-
 Arabidopsis thaliana ?
-
 ?
2.3.1.199 stearoyl-CoA + malonyl-CoA
-
 Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.199 ? x * 43000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.1.199 FAE1 beta-ketoacyl-CoA synthase
-
 Arabidopsis thaliana
2.3.1.199 FAE1 KCS
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.199 6.6 7.5
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.199 additional information CoA, NADPH and ATP have no effect on the condensation activity of the recombinant enzyme Arabidopsis thaliana