EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.7.11 | expression in Escherichia coli, wild-type recombinant enzyme is indistinguishable from the natural one in every criterion investigated | Sulfolobus sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.7.11 | I255L | kcat/Km for pyruvate is 11% of wild-type value, kcat/KM for 2-oxoglutarate is 21% of wild-type value | Sulfolobus sp. |
1.2.7.11 | I255M | kcat/Km for pyruvate is 13% of wild-type value, kcat/KM for 2-oxoglutarate is 2% of wild-type value | Sulfolobus sp. |
1.2.7.11 | I255S | kcat/Km for pyruvate is 23% of wild-type value, kcat/KM for 2-oxoglutarate is 35% of wild-type value | Sulfolobus sp. |
1.2.7.11 | I255V | kcat/Km for pyruvate is 14% of wild-type value, kcat/KM for 2-oxoglutarate is 38% of wild-type value | Sulfolobus sp. |
1.2.7.11 | P254G | kcat/Km for pyruvate is 12% of wild-type value, kcat/KM for 2-oxoglutarate is 20% of wild-type value | Sulfolobus sp. |
1.2.7.11 | P257A | no enzyme activity at either 50 or 80°C | Sulfolobus sp. |
1.2.7.11 | P257G | no enzyme activity at either 50 or 80°C | Sulfolobus sp. |
1.2.7.11 | P257V | no enzyme activity at either 50 or 80°C | Sulfolobus sp. |
1.2.7.11 | T256A | kcat and Km for 2-oxoglutarate are 33% and 51%, respectively, as compared with that of the wild-type enzyme | Sulfolobus sp. |
1.2.7.11 | T256A | kcat/Km for pyruvate is 21% of wild-type value, kcat/KM for 2-oxoglutarate is 15% of wild-type value | Sulfolobus sp. |
1.2.7.11 | T256S | kcat/Km for pyruvate is 15% of wild-type value, kcat/KM for 2-oxoglutarate is 92% of wild-type value | Sulfolobus sp. |
1.2.7.11 | T256V | kcat/Km for pyruvate is 17% of wild-type value, kcat/KM for 2-oxoglutarate is 68% of wild-type value | Sulfolobus sp. |
1.2.7.11 | Y253A | no enzyme activity at either 50 or 80°C | Sulfolobus sp. |
1.2.7.11 | Y253F | kcat/Km for pyruvate is 3.9% of wild-type value, kcat/KM for 2-oxoglutarate is 16% of wild-type value | Sulfolobus sp. |
1.2.7.11 | Y253W | no enzyme activity at either 50 or 80°C | Sulfolobus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.11 | 0.11 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme T256S | Sulfolobus sp. | |
1.2.7.11 | 0.13 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255S | Sulfolobus sp. | |
1.2.7.11 | 0.14 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme T256A | Sulfolobus sp. | |
1.2.7.11 | 0.17 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme P254G | Sulfolobus sp. | |
1.2.7.11 | 0.17 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme T256V | Sulfolobus sp. | |
1.2.7.11 | 0.19 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255M | Sulfolobus sp. | |
1.2.7.11 | 0.25 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255V | Sulfolobus sp. | |
1.2.7.11 | 0.28 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255L | Sulfolobus sp. | |
1.2.7.11 | 0.28 | - |
pyruvate | pH 7.5, 80°C, naturale enzyme | Sulfolobus sp. | |
1.2.7.11 | 0.31 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme P253F | Sulfolobus sp. | |
1.2.7.11 | 0.33 | - |
pyruvate | pH 7.5, 80°C, recombinant enzyme | Sulfolobus sp. | |
1.2.7.11 | 0.33 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme T256V | Sulfolobus sp. | |
1.2.7.11 | 0.37 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme T256A | Sulfolobus sp. | |
1.2.7.11 | 0.72 | - |
2-oxoglutarate | pH 7.5, 80°C, recombinant enzyme | Sulfolobus sp. | |
1.2.7.11 | 0.85 | - |
2-oxoglutarate | pH 7.5, 80°C, natural enzyme | Sulfolobus sp. | |
1.2.7.11 | 0.89 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255S | Sulfolobus sp. | |
1.2.7.11 | 1.1 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255L | Sulfolobus sp. | |
1.2.7.11 | 1.2 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme P254G | Sulfolobus sp. | |
1.2.7.11 | 1.3 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255M | Sulfolobus sp. | |
1.2.7.11 | 1.5 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme P253F | Sulfolobus sp. | |
1.2.7.11 | 2.1 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme T256S | Sulfolobus sp. | |
1.2.7.11 | 3.3 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255V | Sulfolobus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.11 | Mg2+ | natural enzyme: 1.4 mol/mol, recombinant enzyme 1.9 mol/mol | Sulfolobus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.11 | 34000 | - |
1 * 71000 (subunit a) + 1 * 34000 (subunit b), SDS-PAGE | Sulfolobus sp. |
1.2.7.11 | 71000 | - |
1 * 71000 (subunit a) + 1 * 34000 (subunit b), SDS-PAGE | Sulfolobus sp. |
1.2.7.11 | 105000 | - |
gel filtration | Sulfolobus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.11 | Sulfolobus sp. | - |
- |
- |
1.2.7.11 | Sulfolobus sp. 7 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.11 | - |
Sulfolobus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.11 | 2-oxoglutarate + CoA + 2 oxidized ferredoxin | kcat/Km for 2-oxobutyrate is 12% of the kcat/Km-value for pyruvate, a cognate Zn-7Fe-ferredoxin serves as an electron acceptor | Sulfolobus sp. | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
1.2.7.11 | 2-oxoglutarate + CoA + 2 oxidized ferredoxin | kcat/Km for 2-oxobutyrate is 12% of the kcat/Km-value for pyruvate, a cognate Zn-7Fe-ferredoxin serves as an electron acceptor | Sulfolobus sp. 7 | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
1.2.7.11 | additional information | the YPITP-motif is essential for the turnover of the reaction rather than the affinity toward 2-oxoacid | Sulfolobus sp. | ? | - |
? | |
1.2.7.11 | additional information | the YPITP-motif is essential for the turnover of the reaction rather than the affinity toward 2-oxoacid | Sulfolobus sp. 7 | ? | - |
? | |
1.2.7.11 | pyruvate + CoA + 2 oxidized ferredoxin | a cognate Zn-7Fe-ferredoxin serves as an electron acceptor | Sulfolobus sp. | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
1.2.7.11 | pyruvate + CoA + 2 oxidized ferredoxin | a cognate Zn-7Fe-ferredoxin serves as an electron acceptor | Sulfolobus sp. 7 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.11 | dimer | 1 * 71000 (subunit a) + 1 * 34000 (subunit b), SDS-PAGE | Sulfolobus sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.11 | OFOR | - |
Sulfolobus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.11 | 80 | - |
assay at | Sulfolobus sp. |
1.2.7.11 | 90 | - |
- |
Sulfolobus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.7.11 | 70 | - |
pH 7.2, 30 min, about 20% loss of activity, natural and recombinant wild-type enzyme | Sulfolobus sp. |
1.2.7.11 | 80 | - |
pH 7.2, 30 min, about 40% loss of activity, natural and recombinant wild-type enzyme | Sulfolobus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.11 | 1.7 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255V | Sulfolobus sp. | |
1.2.7.11 | 2.2 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme P253F | Sulfolobus sp. | |
1.2.7.11 | 3.2 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255S | Sulfolobus sp. | |
1.2.7.11 | 3.6 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme P254G | Sulfolobus sp. | |
1.2.7.11 | 4.1 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme T256S | Sulfolobus sp. | |
1.2.7.11 | 4.3 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme T256A | Sulfolobus sp. | |
1.2.7.11 | 4.6 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme T256V | Sulfolobus sp. | |
1.2.7.11 | 5.1 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255L | Sulfolobus sp. | |
1.2.7.11 | 5.2 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme P254G | Sulfolobus sp. | |
1.2.7.11 | 5.3 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme P253F | Sulfolobus sp. | |
1.2.7.11 | 5.5 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme T256A | Sulfolobus sp. | |
1.2.7.11 | 5.7 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255L | Sulfolobus sp. | |
1.2.7.11 | 6 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255V | Sulfolobus sp. | |
1.2.7.11 | 6.7 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme T256V | Sulfolobus sp. | |
1.2.7.11 | 7.4 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255S | Sulfolobus sp. | |
1.2.7.11 | 7.4 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme T256S | Sulfolobus sp. | |
1.2.7.11 | 7.9 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255M | Sulfolobus sp. | |
1.2.7.11 | 10 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255M | Sulfolobus sp. | |
1.2.7.11 | 17 | - |
2-oxoglutarate | pH 7.5, 80°C, recombinant enzyme | Sulfolobus sp. | |
1.2.7.11 | 19 | - |
2-oxoglutarate | pH 7.5, 80°C, natural enzyme | Sulfolobus sp. | |
1.2.7.11 | 48 | - |
pyruvate | pH 7.5, 80°C, recombinant enzyme | Sulfolobus sp. | |
1.2.7.11 | 51 | - |
pyruvate | pH 7.5, 80°C, naturale enzyme | Sulfolobus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.7.11 | 7.5 | - |
assay at | Sulfolobus sp. |
1.2.7.11 | 8.5 | - |
- |
Sulfolobus sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.7.11 | thiamine diphosphate | - |
Sulfolobus sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.1 | 7.2 | - |
pyruvate | 80°C, pH 7.5, mutant enzyme Y253F | Sulfolobus sp. | |
1.2.7.1 | 20 | - |
pyruvate | 80°C, pH 7.5, mutant enzyme I255L | Sulfolobus sp. | |
1.2.7.1 | 21 | - |
pyruvate | 80°C, pH 7.5, mutant enzyme P254G | Sulfolobus sp. | |
1.2.7.1 | 24 | - |
pyruvate | 80°C, pH 7.5, mutant enzyme I255V | Sulfolobus sp. | |
1.2.7.1 | 25 | - |
pyruvate | 80°C, pH 7.5, mutant enzyme I255S | Sulfolobus sp. | |
1.2.7.1 | 28 | - |
pyruvate | 80°C, pH 7.5, mutant enzyme T256V | Sulfolobus sp. | |
1.2.7.1 | 31 | - |
pyruvate | 80°C, pH 7.5, mutant enzyme T256A | Sulfolobus sp. | |
1.2.7.1 | 38 | - |
pyruvate | 80°C, pH 7.5, mutant enzyme T256S | Sulfolobus sp. | |
1.2.7.1 | 41 | - |
pyruvate | 80°C, pH 7.5, mutant enzyme I255M | Sulfolobus sp. | |
1.2.7.1 | 145.5 | - |
pyruvate | 80°C, pH 7.5, recombinant enzyme | Sulfolobus sp. | |
1.2.7.1 | 204 | - |
pyruvate | 80°C, pH 7.5, natural enzyme | Sulfolobus sp. | |
1.2.7.3 | 0.52 | - |
2-oxoglutarate | 80°C, pH 7.5, mutant enzyme I255V | Sulfolobus sp. | |
1.2.7.3 | 3.4 | - |
2-oxoglutarate | 80°C, pH 7.5, mutant enzyme T256S | Sulfolobus sp. | |
1.2.7.3 | 3.6 | - |
2-oxoglutarate | 80°C, pH 7.5, mutant enzyme Y253F | Sulfolobus sp. | |
1.2.7.3 | 4.3 | - |
2-oxoglutarate | 80°C, pH 7.5, mutant enzyme P254G | Sulfolobus sp. | |
1.2.7.3 | 4.6 | - |
2-oxoglutarate | 80°C, pH 7.5, mutant enzyme I255L | Sulfolobus sp. | |
1.2.7.3 | 7.7 | - |
2-oxoglutarate | 80°C, pH 7.5, mutant enzyme I255M | Sulfolobus sp. | |
1.2.7.3 | 8.3 | - |
2-oxoglutarate | 80°C, pH 7.5, mutant enzyme I255S | Sulfolobus sp. | |
1.2.7.3 | 15 | - |
2-oxoglutarate | 80°C, pH 7.5, mutant enzyme T256A | Sulfolobus sp. | |
1.2.7.3 | 20 | - |
2-oxoglutarate | 80°C, pH 7.5, mutant enzyme T256V | Sulfolobus sp. | |
1.2.7.3 | 21.8 | - |
2-oxoglutarate | 80°C, pH 7.5, natural enzyme | Sulfolobus sp. | |
1.2.7.3 | 23.6 | - |
2-oxoglutarate | 80°C, pH 7.5, recombinant enzyme | Sulfolobus sp. | |
1.2.7.11 | 0.52 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255V | Sulfolobus sp. | |
1.2.7.11 | 3.4 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme T256S | Sulfolobus sp. | |
1.2.7.11 | 3.6 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme P253F | Sulfolobus sp. | |
1.2.7.11 | 4.3 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme P254G | Sulfolobus sp. | |
1.2.7.11 | 4.6 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255L | Sulfolobus sp. | |
1.2.7.11 | 7.2 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme P253F | Sulfolobus sp. | |
1.2.7.11 | 7.7 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255M | Sulfolobus sp. | |
1.2.7.11 | 8.3 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme I255S | Sulfolobus sp. | |
1.2.7.11 | 15 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme T256A | Sulfolobus sp. | |
1.2.7.11 | 20 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255L | Sulfolobus sp. | |
1.2.7.11 | 20 | - |
2-oxoglutarate | pH 7.5, 80°C, mutant enzyme T256V | Sulfolobus sp. | |
1.2.7.11 | 21 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme P254G | Sulfolobus sp. | |
1.2.7.11 | 22 | - |
2-oxoglutarate | pH 7.5, 80°C, natural enzyme | Sulfolobus sp. | |
1.2.7.11 | 23 | - |
2-oxoglutarate | pH 7.5, 80°C, recombinant enzyme | Sulfolobus sp. | |
1.2.7.11 | 24 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255V | Sulfolobus sp. | |
1.2.7.11 | 25 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255S | Sulfolobus sp. | |
1.2.7.11 | 28 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme T256V | Sulfolobus sp. | |
1.2.7.11 | 31 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme T256A | Sulfolobus sp. | |
1.2.7.11 | 38 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme T256S | Sulfolobus sp. | |
1.2.7.11 | 41 | - |
pyruvate | pH 7.5, 80°C, mutant enzyme I255M | Sulfolobus sp. | |
1.2.7.11 | 146 | - |
pyruvate | pH 7.5, 80°C, recombinant enzyme | Sulfolobus sp. | |
1.2.7.11 | 182 | - |
pyruvate | pH 7.5, 80°C, naturale enzyme | Sulfolobus sp. |