BRENDA - Enzyme Database show

Role of a highly conserved YPITP motif in 2-oxoacid:ferredoxin oxidoreductase: heterologous expression of the gene from Sulfolobus sp.strain 7, and characterization of the recombinant and variant enzymes

Fukuda, E.; Kino, H.; Matsuzawa, H.; Wakagi, T.; Eur. J. Biochem. 268, 5639-5646 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.7.11
expression in Escherichia coli, wild-type recombinant enzyme is indistinguishable from the natural one in every criterion investigated
Sulfolobus sp.
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.2.7.11
I255L
kcat/Km for pyruvate is 11% of wild-type value, kcat/KM for 2-oxoglutarate is 21% of wild-type value
Sulfolobus sp.
1.2.7.11
I255M
kcat/Km for pyruvate is 13% of wild-type value, kcat/KM for 2-oxoglutarate is 2% of wild-type value
Sulfolobus sp.
1.2.7.11
I255S
kcat/Km for pyruvate is 23% of wild-type value, kcat/KM for 2-oxoglutarate is 35% of wild-type value
Sulfolobus sp.
1.2.7.11
I255V
kcat/Km for pyruvate is 14% of wild-type value, kcat/KM for 2-oxoglutarate is 38% of wild-type value
Sulfolobus sp.
1.2.7.11
P254G
kcat/Km for pyruvate is 12% of wild-type value, kcat/KM for 2-oxoglutarate is 20% of wild-type value
Sulfolobus sp.
1.2.7.11
P257A
no enzyme activity at either 50 or 80C
Sulfolobus sp.
1.2.7.11
P257G
no enzyme activity at either 50 or 80C
Sulfolobus sp.
1.2.7.11
P257V
no enzyme activity at either 50 or 80C
Sulfolobus sp.
1.2.7.11
T256A
kcat and Km for 2-oxoglutarate are 33% and 51%, respectively, as compared with that of the wild-type enzyme; kcat/Km for pyruvate is 21% of wild-type value, kcat/KM for 2-oxoglutarate is 15% of wild-type value
Sulfolobus sp.
1.2.7.11
T256S
kcat/Km for pyruvate is 15% of wild-type value, kcat/KM for 2-oxoglutarate is 92% of wild-type value
Sulfolobus sp.
1.2.7.11
T256V
kcat/Km for pyruvate is 17% of wild-type value, kcat/KM for 2-oxoglutarate is 68% of wild-type value
Sulfolobus sp.
1.2.7.11
Y253A
no enzyme activity at either 50 or 80C
Sulfolobus sp.
1.2.7.11
Y253F
kcat/Km for pyruvate is 3.9% of wild-type value, kcat/KM for 2-oxoglutarate is 16% of wild-type value
Sulfolobus sp.
1.2.7.11
Y253W
no enzyme activity at either 50 or 80C
Sulfolobus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.11
0.11
-
pyruvate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
0.13
-
pyruvate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
0.14
-
pyruvate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
0.17
-
pyruvate
pH 7.5, 80C, mutant enzyme P254G; pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
0.19
-
pyruvate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
0.25
-
pyruvate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
0.28
-
pyruvate
pH 7.5, 80C, mutant enzyme I255L; pH 7.5, 80C, naturale enzyme
Sulfolobus sp.
1.2.7.11
0.31
-
pyruvate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
0.33
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
0.33
-
pyruvate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
0.37
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
0.72
-
2-oxoglutarate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
0.85
-
2-oxoglutarate
pH 7.5, 80C, natural enzyme
Sulfolobus sp.
1.2.7.11
0.89
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
1.1
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
1.2
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
1.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
1.5
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
2.1
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
3.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.11
Mg2+
natural enzyme: 1.4 mol/mol, recombinant enzyme 1.9 mol/mol
Sulfolobus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.11
34000
-
1 * 71000 (subunit a) + 1 * 34000 (subunit b), SDS-PAGE
Sulfolobus sp.
1.2.7.11
71000
-
1 * 71000 (subunit a) + 1 * 34000 (subunit b), SDS-PAGE
Sulfolobus sp.
1.2.7.11
105000
-
gel filtration
Sulfolobus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.11
Sulfolobus sp.
-
-
-
1.2.7.11
Sulfolobus sp. 7
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.11
-
Sulfolobus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.11
2-oxoglutarate + CoA + 2 oxidized ferredoxin
kcat/Km for 2-oxobutyrate is 12% of the kcat/Km-value for pyruvate, a cognate Zn-7Fe-ferredoxin serves as an electron acceptor
719399
Sulfolobus sp.
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
2-oxoglutarate + CoA + 2 oxidized ferredoxin
kcat/Km for 2-oxobutyrate is 12% of the kcat/Km-value for pyruvate, a cognate Zn-7Fe-ferredoxin serves as an electron acceptor
719399
Sulfolobus sp. 7
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
additional information
the YPITP-motif is essential for the turnover of the reaction rather than the affinity toward 2-oxoacid
719399
Sulfolobus sp.
?
-
-
-
-
1.2.7.11
additional information
the YPITP-motif is essential for the turnover of the reaction rather than the affinity toward 2-oxoacid
719399
Sulfolobus sp. 7
?
-
-
-
-
1.2.7.11
pyruvate + CoA + 2 oxidized ferredoxin
a cognate Zn-7Fe-ferredoxin serves as an electron acceptor
719399
Sulfolobus sp.
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
pyruvate + CoA + 2 oxidized ferredoxin
a cognate Zn-7Fe-ferredoxin serves as an electron acceptor
719399
Sulfolobus sp. 7
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.7.11
dimer
1 * 71000 (subunit a) + 1 * 34000 (subunit b), SDS-PAGE
Sulfolobus sp.
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.7.11
80
-
assay at
Sulfolobus sp.
1.2.7.11
90
-
-
Sulfolobus sp.
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.2.7.11
70
-
pH 7.2, 30 min, about 20% loss of activity, natural and recombinant wild-type enzyme
Sulfolobus sp.
1.2.7.11
80
-
pH 7.2, 30 min, about 40% loss of activity, natural and recombinant wild-type enzyme
Sulfolobus sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.7.11
1.7
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
2.2
-
pyruvate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
3.2
-
pyruvate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
3.6
-
pyruvate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
4.1
-
pyruvate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
4.3
-
pyruvate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
4.6
-
pyruvate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
5.1
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
5.2
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
5.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
5.5
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
5.7
-
pyruvate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
6
-
pyruvate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
6.7
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
7.4
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255S; pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
7.9
-
pyruvate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
10
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
17
-
2-oxoglutarate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
19
-
2-oxoglutarate
pH 7.5, 80C, natural enzyme
Sulfolobus sp.
1.2.7.11
48
-
pyruvate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
51
-
pyruvate
pH 7.5, 80C, naturale enzyme
Sulfolobus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.11
7.5
-
assay at
Sulfolobus sp.
1.2.7.11
8.5
-
-
Sulfolobus sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.7.11
thiamine diphosphate
-
Sulfolobus sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.11
expression in Escherichia coli, wild-type recombinant enzyme is indistinguishable from the natural one in every criterion investigated
Sulfolobus sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.7.11
thiamine diphosphate
-
Sulfolobus sp.
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.2.7.11
I255L
kcat/Km for pyruvate is 11% of wild-type value, kcat/KM for 2-oxoglutarate is 21% of wild-type value
Sulfolobus sp.
1.2.7.11
I255M
kcat/Km for pyruvate is 13% of wild-type value, kcat/KM for 2-oxoglutarate is 2% of wild-type value
Sulfolobus sp.
1.2.7.11
I255S
kcat/Km for pyruvate is 23% of wild-type value, kcat/KM for 2-oxoglutarate is 35% of wild-type value
Sulfolobus sp.
1.2.7.11
I255V
kcat/Km for pyruvate is 14% of wild-type value, kcat/KM for 2-oxoglutarate is 38% of wild-type value
Sulfolobus sp.
1.2.7.11
P254G
kcat/Km for pyruvate is 12% of wild-type value, kcat/KM for 2-oxoglutarate is 20% of wild-type value
Sulfolobus sp.
1.2.7.11
P257A
no enzyme activity at either 50 or 80C
Sulfolobus sp.
1.2.7.11
P257G
no enzyme activity at either 50 or 80C
Sulfolobus sp.
1.2.7.11
P257V
no enzyme activity at either 50 or 80C
Sulfolobus sp.
1.2.7.11
T256A
kcat and Km for 2-oxoglutarate are 33% and 51%, respectively, as compared with that of the wild-type enzyme; kcat/Km for pyruvate is 21% of wild-type value, kcat/KM for 2-oxoglutarate is 15% of wild-type value
Sulfolobus sp.
1.2.7.11
T256S
kcat/Km for pyruvate is 15% of wild-type value, kcat/KM for 2-oxoglutarate is 92% of wild-type value
Sulfolobus sp.
1.2.7.11
T256V
kcat/Km for pyruvate is 17% of wild-type value, kcat/KM for 2-oxoglutarate is 68% of wild-type value
Sulfolobus sp.
1.2.7.11
Y253A
no enzyme activity at either 50 or 80C
Sulfolobus sp.
1.2.7.11
Y253F
kcat/Km for pyruvate is 3.9% of wild-type value, kcat/KM for 2-oxoglutarate is 16% of wild-type value
Sulfolobus sp.
1.2.7.11
Y253W
no enzyme activity at either 50 or 80C
Sulfolobus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.7.11
0.11
-
pyruvate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
0.13
-
pyruvate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
0.14
-
pyruvate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
0.17
-
pyruvate
pH 7.5, 80C, mutant enzyme P254G; pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
0.19
-
pyruvate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
0.25
-
pyruvate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
0.28
-
pyruvate
pH 7.5, 80C, mutant enzyme I255L; pH 7.5, 80C, naturale enzyme
Sulfolobus sp.
1.2.7.11
0.31
-
pyruvate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
0.33
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
0.33
-
pyruvate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
0.37
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
0.72
-
2-oxoglutarate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
0.85
-
2-oxoglutarate
pH 7.5, 80C, natural enzyme
Sulfolobus sp.
1.2.7.11
0.89
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
1.1
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
1.2
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
1.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
1.5
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
2.1
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
3.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.11
Mg2+
natural enzyme: 1.4 mol/mol, recombinant enzyme 1.9 mol/mol
Sulfolobus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.11
34000
-
1 * 71000 (subunit a) + 1 * 34000 (subunit b), SDS-PAGE
Sulfolobus sp.
1.2.7.11
71000
-
1 * 71000 (subunit a) + 1 * 34000 (subunit b), SDS-PAGE
Sulfolobus sp.
1.2.7.11
105000
-
gel filtration
Sulfolobus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.11
-
Sulfolobus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.11
2-oxoglutarate + CoA + 2 oxidized ferredoxin
kcat/Km for 2-oxobutyrate is 12% of the kcat/Km-value for pyruvate, a cognate Zn-7Fe-ferredoxin serves as an electron acceptor
719399
Sulfolobus sp.
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
2-oxoglutarate + CoA + 2 oxidized ferredoxin
kcat/Km for 2-oxobutyrate is 12% of the kcat/Km-value for pyruvate, a cognate Zn-7Fe-ferredoxin serves as an electron acceptor
719399
Sulfolobus sp. 7
succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
additional information
the YPITP-motif is essential for the turnover of the reaction rather than the affinity toward 2-oxoacid
719399
Sulfolobus sp.
?
-
-
-
-
1.2.7.11
additional information
the YPITP-motif is essential for the turnover of the reaction rather than the affinity toward 2-oxoacid
719399
Sulfolobus sp. 7
?
-
-
-
-
1.2.7.11
pyruvate + CoA + 2 oxidized ferredoxin
a cognate Zn-7Fe-ferredoxin serves as an electron acceptor
719399
Sulfolobus sp.
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
1.2.7.11
pyruvate + CoA + 2 oxidized ferredoxin
a cognate Zn-7Fe-ferredoxin serves as an electron acceptor
719399
Sulfolobus sp. 7
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.7.11
dimer
1 * 71000 (subunit a) + 1 * 34000 (subunit b), SDS-PAGE
Sulfolobus sp.
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.7.11
80
-
assay at
Sulfolobus sp.
1.2.7.11
90
-
-
Sulfolobus sp.
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.2.7.11
70
-
pH 7.2, 30 min, about 20% loss of activity, natural and recombinant wild-type enzyme
Sulfolobus sp.
1.2.7.11
80
-
pH 7.2, 30 min, about 40% loss of activity, natural and recombinant wild-type enzyme
Sulfolobus sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.2.7.11
1.7
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
2.2
-
pyruvate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
3.2
-
pyruvate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
3.6
-
pyruvate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
4.1
-
pyruvate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
4.3
-
pyruvate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
4.6
-
pyruvate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
5.1
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
5.2
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
5.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
5.5
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
5.7
-
pyruvate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
6
-
pyruvate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
6.7
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
7.4
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255S; pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
7.9
-
pyruvate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
10
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
17
-
2-oxoglutarate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
19
-
2-oxoglutarate
pH 7.5, 80C, natural enzyme
Sulfolobus sp.
1.2.7.11
48
-
pyruvate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
51
-
pyruvate
pH 7.5, 80C, naturale enzyme
Sulfolobus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.7.11
7.5
-
assay at
Sulfolobus sp.
1.2.7.11
8.5
-
-
Sulfolobus sp.
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.2.7.1
7.2
-
pyruvate
80C, pH 7.5, mutant enzyme Y253F
Sulfolobus sp.
1.2.7.1
20
-
pyruvate
80C, pH 7.5, mutant enzyme I255L
Sulfolobus sp.
1.2.7.1
21
-
pyruvate
80C, pH 7.5, mutant enzyme P254G
Sulfolobus sp.
1.2.7.1
24
-
pyruvate
80C, pH 7.5, mutant enzyme I255V
Sulfolobus sp.
1.2.7.1
25
-
pyruvate
80C, pH 7.5, mutant enzyme I255S
Sulfolobus sp.
1.2.7.1
28
-
pyruvate
80C, pH 7.5, mutant enzyme T256V
Sulfolobus sp.
1.2.7.1
31
-
pyruvate
80C, pH 7.5, mutant enzyme T256A
Sulfolobus sp.
1.2.7.1
38
-
pyruvate
80C, pH 7.5, mutant enzyme T256S
Sulfolobus sp.
1.2.7.1
41
-
pyruvate
80C, pH 7.5, mutant enzyme I255M
Sulfolobus sp.
1.2.7.1
145.5
-
pyruvate
80C, pH 7.5, recombinant enzyme
Sulfolobus sp.
1.2.7.1
204
-
pyruvate
80C, pH 7.5, natural enzyme
Sulfolobus sp.
1.2.7.3
0.52
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme I255V
Sulfolobus sp.
1.2.7.3
3.4
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme T256S
Sulfolobus sp.
1.2.7.3
3.6
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme Y253F
Sulfolobus sp.
1.2.7.3
4.3
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme P254G
Sulfolobus sp.
1.2.7.3
4.6
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme I255L
Sulfolobus sp.
1.2.7.3
7.7
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme I255M
Sulfolobus sp.
1.2.7.3
8.3
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme I255S
Sulfolobus sp.
1.2.7.3
15
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme T256A
Sulfolobus sp.
1.2.7.3
20
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme T256V
Sulfolobus sp.
1.2.7.3
21.8
-
2-oxoglutarate
80C, pH 7.5, natural enzyme
Sulfolobus sp.
1.2.7.3
23.6
-
2-oxoglutarate
80C, pH 7.5, recombinant enzyme
Sulfolobus sp.
1.2.7.11
0.52
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
3.4
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
3.6
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
4.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
4.6
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
7.2
-
pyruvate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
7.7
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
8.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
15
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
20
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
20
-
pyruvate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
21
-
pyruvate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
22
-
2-oxoglutarate
pH 7.5, 80C, natural enzyme
Sulfolobus sp.
1.2.7.11
23
-
2-oxoglutarate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
24
-
pyruvate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
25
-
pyruvate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
28
-
pyruvate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
31
-
pyruvate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
38
-
pyruvate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
41
-
pyruvate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
146
-
pyruvate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
182
-
pyruvate
pH 7.5, 80C, naturale enzyme
Sulfolobus sp.
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.2.7.1
7.2
-
pyruvate
80C, pH 7.5, mutant enzyme Y253F
Sulfolobus sp.
1.2.7.1
20
-
pyruvate
80C, pH 7.5, mutant enzyme I255L
Sulfolobus sp.
1.2.7.1
21
-
pyruvate
80C, pH 7.5, mutant enzyme P254G
Sulfolobus sp.
1.2.7.1
24
-
pyruvate
80C, pH 7.5, mutant enzyme I255V
Sulfolobus sp.
1.2.7.1
25
-
pyruvate
80C, pH 7.5, mutant enzyme I255S
Sulfolobus sp.
1.2.7.1
28
-
pyruvate
80C, pH 7.5, mutant enzyme T256V
Sulfolobus sp.
1.2.7.1
31
-
pyruvate
80C, pH 7.5, mutant enzyme T256A
Sulfolobus sp.
1.2.7.1
38
-
pyruvate
80C, pH 7.5, mutant enzyme T256S
Sulfolobus sp.
1.2.7.1
41
-
pyruvate
80C, pH 7.5, mutant enzyme I255M
Sulfolobus sp.
1.2.7.1
145.5
-
pyruvate
80C, pH 7.5, recombinant enzyme
Sulfolobus sp.
1.2.7.1
204
-
pyruvate
80C, pH 7.5, natural enzyme
Sulfolobus sp.
1.2.7.3
0.52
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme I255V
Sulfolobus sp.
1.2.7.3
3.4
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme T256S
Sulfolobus sp.
1.2.7.3
3.6
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme Y253F
Sulfolobus sp.
1.2.7.3
4.3
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme P254G
Sulfolobus sp.
1.2.7.3
4.6
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme I255L
Sulfolobus sp.
1.2.7.3
7.7
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme I255M
Sulfolobus sp.
1.2.7.3
8.3
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme I255S
Sulfolobus sp.
1.2.7.3
15
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme T256A
Sulfolobus sp.
1.2.7.3
20
-
2-oxoglutarate
80C, pH 7.5, mutant enzyme T256V
Sulfolobus sp.
1.2.7.3
21.8
-
2-oxoglutarate
80C, pH 7.5, natural enzyme
Sulfolobus sp.
1.2.7.3
23.6
-
2-oxoglutarate
80C, pH 7.5, recombinant enzyme
Sulfolobus sp.
1.2.7.11
0.52
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
3.4
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
3.6
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
4.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
4.6
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
7.2
-
pyruvate
pH 7.5, 80C, mutant enzyme P253F
Sulfolobus sp.
1.2.7.11
7.7
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
8.3
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
15
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
20
-
2-oxoglutarate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
20
-
pyruvate
pH 7.5, 80C, mutant enzyme I255L
Sulfolobus sp.
1.2.7.11
21
-
pyruvate
pH 7.5, 80C, mutant enzyme P254G
Sulfolobus sp.
1.2.7.11
22
-
2-oxoglutarate
pH 7.5, 80C, natural enzyme
Sulfolobus sp.
1.2.7.11
23
-
2-oxoglutarate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
24
-
pyruvate
pH 7.5, 80C, mutant enzyme I255V
Sulfolobus sp.
1.2.7.11
25
-
pyruvate
pH 7.5, 80C, mutant enzyme I255S
Sulfolobus sp.
1.2.7.11
28
-
pyruvate
pH 7.5, 80C, mutant enzyme T256V
Sulfolobus sp.
1.2.7.11
31
-
pyruvate
pH 7.5, 80C, mutant enzyme T256A
Sulfolobus sp.
1.2.7.11
38
-
pyruvate
pH 7.5, 80C, mutant enzyme T256S
Sulfolobus sp.
1.2.7.11
41
-
pyruvate
pH 7.5, 80C, mutant enzyme I255M
Sulfolobus sp.
1.2.7.11
146
-
pyruvate
pH 7.5, 80C, recombinant enzyme
Sulfolobus sp.
1.2.7.11
182
-
pyruvate
pH 7.5, 80C, naturale enzyme
Sulfolobus sp.