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Literature summary extracted from

  • Cho, H.K.; Kim, H.H.; Seo, D.H.; Jung, J.H.; Park, J.H.; Baek, N.I.; Kim, M.J.; Yoo, S.H.; Cha, J.; Kim, Y.R.; Park, C.S.
    Biosynthesis of (+)-catechin glycosides using recombinant amylosucrase from Deinococcus geothermalis DSM 11300 (2011), Enzyme Microb. Technol., 49, 246-253.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.4 gene dgas, expression of GST-tagged DGAS in Escherichia coli Deinococcus geothermalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n Deinococcus geothermalis
-
 D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n Deinococcus geothermalis DSM 11300
-
 D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Deinococcus geothermalis Q1J0W0 gene dgas
-
2.4.1.4 Deinococcus geothermalis DSM 11300 Q1J0W0 gene dgas
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.4 recombinant GST-tagged DGAS from Escherichia coli by glutathione affinity chromatography Deinococcus geothermalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 additional information NMR product analysis, overview Deinococcus geothermalis ?
-
 ?
2.4.1.4 additional information NMR product analysis, overview Deinococcus geothermalis DSM 11300 ?
-
 ?
2.4.1.4 sucrose + (+)-catechin
-
 Deinococcus geothermalis D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
 ?
2.4.1.4 sucrose + (+)-catechin
-
 Deinococcus geothermalis DSM 11300 D-fructose + (+)-catechin-3'-O-alpha-D-glucopyranoside
-
 ?
2.4.1.4 sucrose + maltose the enzyme also produces (+)-catechin maltooligosaccharides Deinococcus geothermalis D-fructose + maltotriose
-
 ?
2.4.1.4 sucrose + maltose the enzyme also produces (+)-catechin maltooligosaccharides Deinococcus geothermalis DSM 11300 D-fructose + maltotriose
-
 ?
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n
-
 Deinococcus geothermalis D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity Deinococcus geothermalis D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n
-
 Deinococcus geothermalis DSM 11300 D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.4 sucrose + [(1->4)-alpha-D-glucosyl]n a phenolic aglycone compound can also act an acceptor molecule of ASase transglycosylation activity Deinococcus geothermalis DSM 11300 D-fructose + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.4 ASASE
-
 Deinococcus geothermalis
2.4.1.4 DGAS
-
 Deinococcus geothermalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.4 45
-
 assay at Deinococcus geothermalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.4 7
-
 assay at Deinococcus geothermalis