BRENDA - Enzyme Database

Caenorhabditis elegans NDX-4 is a MutT-type enzyme that contributes to genomic stability

Arczewska, K.D.; Baumeier, C.; Kassahun, H.; Sengupta, T.; Bjoras, M.; Kusmierek, J.T.; Nilsen, H.; DNA Repair 10, 176-187 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.17
expressed in Escherichia coli BL21(DE3)T cells
Caenorhabditis elegans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.007
-
diadenosine 5',5''''-P1,P4-tetraphosphate
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.0986
-
8-oxo-dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.1143
-
dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.182
-
dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.3846
-
2-oxo-dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Caenorhabditis elegans
Q9U2M7
-
-
3.6.1.17
Caenorhabditis elegans Bristol N2
Q9U2M7
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.6.1.17
HisTrap FF column chromatography and Superdex 75 gel filtration
Caenorhabditis elegans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
2-oxo-dATP + H2O
less efficient hydrolysis compared to 8-oxo-dGTP
719339
Caenorhabditis elegans
?
-
-
-
?
3.6.1.17
2-oxo-dATP + H2O
less efficient hydrolysis compared to 8-oxo-dGTP
719339
Caenorhabditis elegans Bristol N2
?
-
-
-
?
3.6.1.17
8-oxo-dGTP + H2O
the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency
719339
Caenorhabditis elegans
8-oxo-dGMP + diphosphate
-
-
-
?
3.6.1.17
8-oxo-dGTP + H2O
the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency
719339
Caenorhabditis elegans Bristol N2
8-oxo-dGMP + diphosphate
-
-
-
?
3.6.1.17
dATP + H2O
-
719339
Caenorhabditis elegans
?
-
-
-
?
3.6.1.17
dATP + H2O
-
719339
Caenorhabditis elegans Bristol N2
?
-
-
-
?
3.6.1.17
dGTP + H2O
-
719339
Caenorhabditis elegans
?
-
-
-
?
3.6.1.17
dGTP + H2O
-
719339
Caenorhabditis elegans Bristol N2
?
-
-
-
?
3.6.1.17
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
-
719339
Caenorhabditis elegans
AMP + ATP
-
-
-
?
3.6.1.17
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
-
719339
Caenorhabditis elegans Bristol N2
AMP + ATP
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.37
-
dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.51
-
2-oxo-dATP
in 5 0mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.53
-
dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.58
-
8-oxo-dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
27
-
diadenosine 5',5''''-P1,P4-tetraphosphate
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
expressed in Escherichia coli BL21(DE3)T cells
Caenorhabditis elegans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.007
-
diadenosine 5',5''''-P1,P4-tetraphosphate
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.0986
-
8-oxo-dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.1143
-
dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.182
-
dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.3846
-
2-oxo-dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
HisTrap FF column chromatography and Superdex 75 gel filtration
Caenorhabditis elegans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
2-oxo-dATP + H2O
less efficient hydrolysis compared to 8-oxo-dGTP
719339
Caenorhabditis elegans
?
-
-
-
?
3.6.1.17
2-oxo-dATP + H2O
less efficient hydrolysis compared to 8-oxo-dGTP
719339
Caenorhabditis elegans Bristol N2
?
-
-
-
?
3.6.1.17
8-oxo-dGTP + H2O
the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency
719339
Caenorhabditis elegans
8-oxo-dGMP + diphosphate
-
-
-
?
3.6.1.17
8-oxo-dGTP + H2O
the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency
719339
Caenorhabditis elegans Bristol N2
8-oxo-dGMP + diphosphate
-
-
-
?
3.6.1.17
dATP + H2O
-
719339
Caenorhabditis elegans
?
-
-
-
?
3.6.1.17
dATP + H2O
-
719339
Caenorhabditis elegans Bristol N2
?
-
-
-
?
3.6.1.17
dGTP + H2O
-
719339
Caenorhabditis elegans
?
-
-
-
?
3.6.1.17
dGTP + H2O
-
719339
Caenorhabditis elegans Bristol N2
?
-
-
-
?
3.6.1.17
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
-
719339
Caenorhabditis elegans
AMP + ATP
-
-
-
?
3.6.1.17
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
-
719339
Caenorhabditis elegans Bristol N2
AMP + ATP
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.37
-
dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.51
-
2-oxo-dATP
in 5 0mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.53
-
dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
0.58
-
8-oxo-dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
27
-
diadenosine 5',5''''-P1,P4-tetraphosphate
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
General Information
EC Number
General Information
Commentary
Organism
3.6.1.17
malfunction
loss of NDX-4 leads to upregulation of key stress responsive genes that likely compensate for the in vivo role of NDX-4 in protection against deleterious consequences of oxidative stress. NDX-4 deficiency protects nematodes from acute heat shock
Caenorhabditis elegans
3.6.1.17
physiological function
the enzyme contributes to genomic stability in vivo in Caenorhabditis elegans
Caenorhabditis elegans
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.6.1.17
malfunction
loss of NDX-4 leads to upregulation of key stress responsive genes that likely compensate for the in vivo role of NDX-4 in protection against deleterious consequences of oxidative stress. NDX-4 deficiency protects nematodes from acute heat shock
Caenorhabditis elegans
3.6.1.17
physiological function
the enzyme contributes to genomic stability in vivo in Caenorhabditis elegans
Caenorhabditis elegans
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.6.1.17
1.3
-
2-oxo-dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
2.9
-
dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
3.3
-
dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
5.9
-
8-oxo-dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
3900
-
diadenosine 5',5''''-P1,P4-tetraphosphate
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.6.1.17
1.3
-
2-oxo-dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
2.9
-
dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
3.3
-
dATP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
5.9
-
8-oxo-dGTP
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans
3.6.1.17
3900
-
diadenosine 5',5''''-P1,P4-tetraphosphate
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
Caenorhabditis elegans