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Literature summary extracted from

  • Arczewska, K.D.; Baumeier, C.; Kassahun, H.; Sengupta, T.; Bjoras, M.; Kusmierek, J.T.; Nilsen, H.
    Caenorhabditis elegans NDX-4 is a MutT-type enzyme that contributes to genomic stability (2011), DNA Repair, 10, 176-187.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.17 expressed in Escherichia coli BL21(DE3)T cells Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.17 0.007
-
 diadenosine 5',5''''-P1,P4-tetraphosphate in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 0.0986
-
 8-oxo-dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 0.1143
-
 dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 0.182
-
 dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 0.3846
-
 2-oxo-dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.17 Caenorhabditis elegans Q9U2M7
-
-
3.6.1.17 Caenorhabditis elegans Bristol N2 Q9U2M7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.17 HisTrap FF column chromatography and Superdex 75 gel filtration Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.17 2-oxo-dATP + H2O less efficient hydrolysis compared to 8-oxo-dGTP Caenorhabditis elegans ?
-
 ?
3.6.1.17 2-oxo-dATP + H2O less efficient hydrolysis compared to 8-oxo-dGTP Caenorhabditis elegans Bristol N2 ?
-
 ?
3.6.1.17 8-oxo-dGTP + H2O the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency Caenorhabditis elegans 8-oxo-dGMP + diphosphate
-
 ?
3.6.1.17 8-oxo-dGTP + H2O the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency Caenorhabditis elegans Bristol N2 8-oxo-dGMP + diphosphate
-
 ?
3.6.1.17 dATP + H2O
-
 Caenorhabditis elegans ?
-
 ?
3.6.1.17 dATP + H2O
-
 Caenorhabditis elegans Bristol N2 ?
-
 ?
3.6.1.17 dGTP + H2O
-
 Caenorhabditis elegans ?
-
 ?
3.6.1.17 dGTP + H2O
-
 Caenorhabditis elegans Bristol N2 ?
-
 ?
3.6.1.17 diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
-
 Caenorhabditis elegans AMP + ATP
-
 ?
3.6.1.17 diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
-
 Caenorhabditis elegans Bristol N2 AMP + ATP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.17 NDX-4
-
 Caenorhabditis elegans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.17 0.37
-
 dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 0.51
-
 2-oxo-dATP in 5 0mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 0.53
-
 dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 0.58
-
 8-oxo-dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 27
-
 diadenosine 5',5''''-P1,P4-tetraphosphate in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
3.6.1.17 malfunction loss of NDX-4 leads to upregulation of key stress responsive genes that likely compensate for the in vivo role of NDX-4 in protection against deleterious consequences of oxidative stress. NDX-4 deficiency protects nematodes from acute heat shock Caenorhabditis elegans
3.6.1.17 physiological function the enzyme contributes to genomic stability in vivo in Caenorhabditis elegans Caenorhabditis elegans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.17 1.3
-
 2-oxo-dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 2.9
-
 dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 3.3
-
 dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 5.9
-
 8-oxo-dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.6.1.17 3900
-
 diadenosine 5',5''''-P1,P4-tetraphosphate in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans