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Literature summary extracted from

  • Eschrich, D.; Buchhaupt, M.; Koetter, P.; Entian, K.D.
    Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis (2002), Curr. Genet., 40, 326-338.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.260 gene NEP1, DNA and amino acid sequence determination and analysis Candida albicans
2.1.1.260 gene NEP1, DNA and amino acid sequence determination and analysis Nakaseomyces glabratus
2.1.1.260 gene NEP1, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the GFP-tagged ScNEP1 in enzyme-deficient Saccharomyces cerevisiae strain CEN.SR679 in the nucleus Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.260 additional information complementation of a ScDnep1 strain with the human Nep1 (C2F) protein, the HsNEP1 open reading frame is expressed with the yeast inducible/repressible GAL1 promoter and the resulting plasmid (pGALHsNEP1) is transformed into the heterozygous ScDnep1/ScNEP1 strain CEN.SR679. After sporulation and tetrad analysis, ScDnep1 segregants complemented by the pGAL-HsNEP1 are only viable with galactose Saccharomyces cerevisiae
2.1.1.260 additional information complementation of a ScDnep1 strain with the human Nep1 (C2F) protein, the HsNEP1 open reading frame is expressed with the yeast inducible/repressible GAL1 promoter and the resulting plasmid (pGALHsNEP1) is transformed into the heterozygous ScDnep1/ScNEP1 strain CEN.SR679. After sporulation and tetrad analysis, ScDnep1 segregants complemented by the pGAL-HsNEP1 are only viable with galactose Homo sapiens
2.1.1.260 additional information construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrations of methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth Candida albicans
2.1.1.260 additional information construction of a heterozygous CaDnep1/CaNEP1 strain CAE8 after replacement of one CaNEP1 wild-type allele with a CaURA3 marker and introduction of GFP-open reading frame driven by the methionine/cysteine-repressible CaMET3 promoter in front of the gene. Without high concentrationsof methionine and cysteine in the medium, the resulting strain is viable, but addition of 2.5 mM methionine and 2.5 mM cysteine strongly impairs growth Nakaseomyces glabratus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.260 microtubule the spindle/microtubule association is specific for ScNep1p Saccharomyces cerevisiae 5874
-
2.1.1.260 nucleolus
-
 Homo sapiens 5730
-
2.1.1.260 nucleolus
-
 Candida albicans 5730
-
2.1.1.260 nucleolus
-
 Nakaseomyces glabratus 5730
-
2.1.1.260 spindle the spindle/microtubule association is specific for ScNep1p Saccharomyces cerevisiae 5819
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.260 28000
-
 x * 28000 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.260 S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
-
 ?
2.1.1.260 S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA Saccharomyces cerevisiae CEN.PK2
-
 S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.260 Candida albicans Q9P8P7 derived from the clinical isolate SC5314, gene NEP1
-
2.1.1.260 Candida albicans RM1000 Q9P8P7 derived from the clinical isolate SC5314, gene NEP1
-
2.1.1.260 Homo sapiens
-
 gene NEP1
-
2.1.1.260 Nakaseomyces glabratus Q96UP2 derived from the clinical isolate SC5314, gene NEP1
-
2.1.1.260 Nakaseomyces glabratus CBS 138 Q96UP2 derived from the clinical isolate SC5314, gene NEP1
-
2.1.1.260 Saccharomyces cerevisiae
-
 and isogenic strains, gene YLR186w or NEP1 or EMG1
-
2.1.1.260 Saccharomyces cerevisiae CEN.PK2
-
 and isogenic strains, gene YLR186w or NEP1 or EMG1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.260 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.260 S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
-
 ?
2.1.1.260 S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
-
 Saccharomyces cerevisiae CEN.PK2 S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.260 ? x * 28000 Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.1.1.260 Emg1p
-
 Saccharomyces cerevisiae
2.1.1.260 Emg1p
-
 Homo sapiens
2.1.1.260 Emg1p
-
 Candida albicans
2.1.1.260 Emg1p
-
 Nakaseomyces glabratus
2.1.1.260 essential for mitotic growth 1
-
 Saccharomyces cerevisiae
2.1.1.260 essential for mitotic growth 1
-
 Homo sapiens
2.1.1.260 essential for mitotic growth 1
-
 Candida albicans
2.1.1.260 essential for mitotic growth 1
-
 Nakaseomyces glabratus
2.1.1.260 Nep1p
-
 Saccharomyces cerevisiae
2.1.1.260 Nep1p
-
 Homo sapiens
2.1.1.260 Nep1p
-
 Candida albicans
2.1.1.260 Nep1p
-
 Nakaseomyces glabratus
2.1.1.260 nucleolar essential protein 1
-
 Saccharomyces cerevisiae
2.1.1.260 nucleolar essential protein 1
-
 Homo sapiens
2.1.1.260 nucleolar essential protein 1
-
 Candida albicans
2.1.1.260 nucleolar essential protein 1
-
 Nakaseomyces glabratus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.260 S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae
2.1.1.260 S-adenosyl-L-methionine
-
 Homo sapiens
2.1.1.260 S-adenosyl-L-methionine
-
 Candida albicans
2.1.1.260 S-adenosyl-L-methionine
-
 Nakaseomyces glabratus

General Information

EC Number General Information Comment Organism
2.1.1.260 malfunction a temperature-sensitive ScNEP1ts1 allele is isolated and reveals a strongly increased sensitivity to paromomycin, a translational inhibitor which binds to RNA, indicating that ribosome biogenesis within the nucleolus is probably affected. Candida albicans and human NEP1 heterologously complement the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant, the ScNEP1 spindle/microtubule phenotype is not found with HsNEP1 and CaNEP1 Saccharomyces cerevisiae
2.1.1.260 additional information human NEP1 heterologously complements the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant Homo sapiens
2.1.1.260 additional information Candida albicans NEP1 heterologously complements the essential phenotype in a Saccharomyces cerevisiae nep1 deletion mutant Candida albicans
2.1.1.260 physiological function the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins. The protein has an essential function in ribosomal biogenesis which directly or indirectly interferes with a methylation reaction during the early steps of pre-rRNA processing necessary for the generation of 40S ribosomal subunits Saccharomyces cerevisiae
2.1.1.260 physiological function the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins Homo sapiens
2.1.1.260 physiological function the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins Candida albicans
2.1.1.260 physiological function the rRNA modifications are thought to play a role in modulation of the three-dimensional structure of RNA and in fine-tuning its interactions with other RNAs or proteins Nakaseomyces glabratus