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Literature summary extracted from

  • Nakayama, T.; Kamachi, T.; Jitsumori, K.; Omi, R.; Hirotsu, K.; Esaki, N.; Kurihara, T.; Yoshizawa, K.
    Substrate specificity of fluoroacetate dehalogenase: an insight from crystallographic analysis, fluorescence spectroscopy, and theoretical computations (2012), Chemistry, 18, 8392-8402.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.8.1.3 mutant D104A in compex with fluoroacetate, X-ray diffraction structure determination and analysis at 1.2 A resolution Burkholderia sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.3 D104A site-directed mutagenesis Burkholderia sp.
3.8.1.3 W150F site-directed mutagenesis Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.3 chloroacetate + H2O Burkholderia sp.
-
 glycolate + chloride
-
 ?
3.8.1.3 fluoroacetate + H2O Burkholderia sp.
-
 glycolate + fluoride
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.3 Burkholderia sp. Q1JU72
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.3 chloroacetate + H2O
-
 Burkholderia sp. glycolate + chloride
-
 ?
3.8.1.3 chloroacetate + H2O substrate binding structure Burkholderia sp. glycolate + chloride
-
 ?
3.8.1.3 fluoroacetate + H2O
-
 Burkholderia sp. glycolate + fluoride
-
 ?
3.8.1.3 fluoroacetate + H2O substrate binding structure Burkholderia sp. glycolate + fluoride
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.8.1.3 fluoroacetate dehalogenase
-
 Burkholderia sp.

General Information

EC Number General Information Comment Organism
3.8.1.3 additional information the enzyme active site is composed of Asp104, Arg105, Arg108, His149, Trp150, Tyr212, and His271, environments of the tryptophan residues are different between the fluoroacetate and chloroacetate-bound enzymes, active site docking simulations and QM/MM optimizations, QM/MM calculations for the defluorination of fluoroacetate, overview Burkholderia sp.