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Literature summary extracted from
- Autodisplay of nitrilase from Alcaligenes faecalis in E. coli yields a whole cell biocatalyst for the synthesis of enantiomerically pure (R)-mandelic Acid (2011), ChemCatChem, 3, 719-725.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.5.5 | recombinant functional expression of the enzyme in the cell envelope of Escherichia coli, where it is accessible for externally added protease | Alcaligenes faecalis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.5 | Alcaligenes faecalis | - |
subsp. faecalis | - |
| 3.5.5.5 | Alcaligenes faecalis ATCC 8750 | - |
subsp. faecalis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.5 | (R,S)-mandelonitrile + 2 H2O | - |
Alcaligenes faecalis | (R)-mandelic acid + NH3 | - |
? |  |
| 3.5.5.5 | (R,S)-mandelonitrile + 2 H2O | - |
Alcaligenes faecalis ATCC 8750 | (R)-mandelic acid + NH3 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.5 | 45 | - |
- |
Alcaligenes faecalis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.5 | 7.5 | - |
- |
Alcaligenes faecalis |
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Release 2023.1 (January 2023)
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