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Literature summary extracted from

  • Wang, J.; Han, X.; Saha, S.; Xu, T.; Rai, R.; Zhang, F.; Wolf, Y.I.; Wolfson, A.; Yates, J.R.; Kashina, A.
    Arginyltransferase is an ATP-independent self-regulating enzyme that forms distinct functional complexes in vivo (2011), Chem. Biol., 18, 121-130.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.8 expressed in Escherichia coli BL21(DE3) cells Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.8 cytosol
-
Mus musculus 5829
-
2.3.2.8 ribosome
-
Mus musculus 5840
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.8 60000
-
x * 60000, isoform ATE1-2, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.8 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.8 Ni-NTA column chromatography Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.8 L-arginyl-tRNAArg + bovine alpha-lactalbumin higher activity is detected with isoforms ATE1-1 (100%) and ATE1-2 (85%), and weaker activity is detected with isoforms ATE1-3 (18%) and ATE1-4 (4%) Mus musculus tRNAArg + L-arginyl-[bovine alpha-lactalbumin]
-
?
2.3.2.8 L-arginyl-tRNAArg + bovine serum albumin high activity is detected with isoforms ATE1-1 (100%) and ATE1-2 (95%), and weaker activity is detected with isoforms ATE1-3 (10%) and ATE1-4 (4%) Mus musculus tRNAArg + L-arginyl-[bovine serum albumin]
-
?
2.3.2.8 additional information ATE1 is capable of self-arginylation in vitro and in vivo Mus musculus ?
-
?
2.3.2.8 additional information the arginylation reaction does not require the formation of an ATE1-arginyl-tRNA synthetase complex or the presence of ATP Mus musculus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.3.2.8 ? x * 60000, isoform ATE1-2, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.2.8 Ate1
-
Mus musculus
2.3.2.8 ATE1-1 isoform Mus musculus
2.3.2.8 ATE1-2 isoform Mus musculus
2.3.2.8 Ate1-3 isoform Mus musculus
2.3.2.8 Ate1-4 isoform Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.2.8 additional information the enzyme is independent of ATP Mus musculus