EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.13 | expression in Escherichia coli | Niallia circulans |
4.1.1.95 | expressed in Escherichia coli BL21(DE3) cells | Niallia circulans |
6.2.1.39 | gene btrJ, butirosin genetic cluster organization, overview. Expression of N-terminal His6-tagged BtrJ | Niallia circulans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.39 | Mg2+ | essentially required | Niallia circulans | |
6.2.1.39 | Mn2+ | essentially required, activates | Niallia circulans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.13 | 38700 | - |
x * 38700, calculated, x * 40000, SDS-PAGE of recombinant His-tagged protein | Niallia circulans |
1.14.14.13 | 40000 | - |
x * 38700, calculated, x * 40000, SDS-PAGE of recombinant His-tagged protein | Niallia circulans |
4.1.1.95 | 47800 | - |
2 * 47800, calculated from amino acid sequence | Niallia circulans |
4.1.1.95 | 49846 | - |
2 * 49846, LC-electrospray ionization mass spectrometry | Niallia circulans |
6.2.1.39 | 48600 | - |
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation | Niallia circulans |
6.2.1.39 | 50962 | - |
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation | Niallia circulans |
6.2.1.39 | 50969 | - |
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation | Niallia circulans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.39 | ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | Niallia circulans | - |
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] | - |
? | |
6.2.1.39 | ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | Niallia circulans NR3312 | - |
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] | - |
? | |
6.2.1.39 | ATP + L-glutamate + BtrI acyl-carrier protein | Niallia circulans | - |
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] | - |
? | |
6.2.1.39 | ATP + L-glutamate + BtrI acyl-carrier protein | Niallia circulans NR3312 | - |
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.13 | Niallia circulans | Q4H4E5 | putative | - |
4.1.1.95 | Niallia circulans | - |
- |
- |
4.1.1.95 | Niallia circulans NR3312 | - |
- |
- |
6.2.1.39 | Niallia circulans | Q4H4E7 | gene btrJ | - |
6.2.1.39 | Niallia circulans NR3312 | Q4H4E7 | gene btrJ | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.95 | Ni2+-NTA affinity column chromatography | Niallia circulans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.13 | 4-(L-gamma-glutamylamino)butanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2 | presence of FMN and O2 are required | Niallia circulans | 4-(L-gamma-glutamylamino)-(2S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] + FMN + H2O | - |
? | |
1.14.14.13 | butanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2 | - |
Niallia circulans | (2S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] + FMN + H2O | - |
? | |
1.14.14.13 | additional information | no substrates: butanoate, butanoyl-CoA, L-glutamate, L-aspartate, gamma-aminobutanoate, and L-ornithine | Niallia circulans | ? | - |
? | |
1.14.14.13 | octanoyl-[BtrI acyl-carrier protein] + FMNH2 + O2 | - |
Niallia circulans | (2S)-2-hydroxyoctanoyl-[BtrI acyl-carrier protein] + FMN + H2O | - |
? | |
4.1.1.95 | diaminopimelate | - |
Niallia circulans | ? | - |
? | |
4.1.1.95 | diaminopimelate | - |
Niallia circulans NR3312 | ? | - |
? | |
4.1.1.95 | gamma-L-Glu-[S-BtrI acyl carrier protein] | - |
Niallia circulans | 4-aminobutanoyl-[S-BtrI acyl carrier protein] + CO2 | - |
? | |
4.1.1.95 | gamma-L-Glu-[S-BtrI acyl carrier protein] | - |
Niallia circulans NR3312 | 4-aminobutanoyl-[S-BtrI acyl carrier protein] + CO2 | - |
? | |
4.1.1.95 | L-arginine | - |
Niallia circulans | ? | - |
? | |
4.1.1.95 | L-lysine | - |
Niallia circulans | ? | - |
? | |
4.1.1.95 | L-lysine | - |
Niallia circulans NR3312 | ? | - |
? | |
4.1.1.95 | L-ornithine | - |
Niallia circulans | ? | - |
? | |
4.1.1.95 | L-ornithine | - |
Niallia circulans NR3312 | ? | - |
? | |
4.1.1.95 | additional information | the enzyme is not able to decarboxylate L-glutamate or any D-amino acids | Niallia circulans | ? | - |
? | |
4.1.1.95 | additional information | the enzyme is not able to decarboxylate L-glutamate or any D-amino acids | Niallia circulans NR3312 | ? | - |
? | |
6.2.1.39 | ATP + 4-aminobutanoate + BtrI acyl-carrier protein | - |
Niallia circulans | ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein] | GABA-S-BtrI is closely related to holo-BtrI | ? | |
6.2.1.39 | ATP + 4-aminobutanoate + BtrI acyl-carrier protein | - |
Niallia circulans NR3312 | ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein] | GABA-S-BtrI is closely related to holo-BtrI | ? | |
6.2.1.39 | ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | - |
Niallia circulans | ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] | - |
? | |
6.2.1.39 | ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | - |
Niallia circulans NR3312 | ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] | - |
? | |
6.2.1.39 | ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | BtrJ is able to catalyze ligation of a second L-glutamate molecule to GABA-S-BtrI, forming an amide bond | Niallia circulans | ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein] | - |
? | |
6.2.1.39 | ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein] | BtrJ is able to catalyze ligation of a second L-glutamate molecule to GABA-S-BtrI, forming an amide bond | Niallia circulans NR3312 | ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein] | - |
? | |
6.2.1.39 | ATP + L-glutamate + BtrI acyl-carrier protein | - |
Niallia circulans | ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] | - |
? | |
6.2.1.39 | ATP + L-glutamate + BtrI acyl-carrier protein | - |
Niallia circulans NR3312 | ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] | - |
? | |
6.2.1.39 | additional information | the reaction mechanism is proposed to involve the generation of an acylphosphate intermediate via hydrolysis of ATP to ADP. No activity with (2S)-4-amino-2-hydroxybutyrate, AHBA. No gamma-L-Glu-gamma-L-Glu-S-BtrI produced in the BtrJ assay | Niallia circulans | ? | - |
? | |
6.2.1.39 | additional information | the reaction mechanism is proposed to involve the generation of an acylphosphate intermediate via hydrolysis of ATP to ADP. No activity with (2S)-4-amino-2-hydroxybutyrate, AHBA. No gamma-L-Glu-gamma-L-Glu-S-BtrI produced in the BtrJ assay | Niallia circulans NR3312 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.13 | ? | x * 38700, calculated, x * 40000, SDS-PAGE of recombinant His-tagged protein | Niallia circulans |
4.1.1.95 | homodimer | 2 * 47800, calculated from amino acid sequence | Niallia circulans |
4.1.1.95 | homodimer | 2 * 49846, LC-electrospray ionization mass spectrometry | Niallia circulans |
6.2.1.39 | monomer | 1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation | Niallia circulans |
6.2.1.39 | More | purified BtrJ shows aggregation in solution, preventable by DTT | Niallia circulans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.13 | btrO | - |
Niallia circulans |
4.1.1.95 | btrK | - |
Niallia circulans |
6.2.1.39 | BtrJ | - |
Niallia circulans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.13 | FMN | - |
Niallia circulans | |
1.14.14.13 | additional information | no cofactor: FAD | Niallia circulans | |
4.1.1.95 | pyridoxal 5'-phosphate | dependent on | Niallia circulans | |
6.2.1.39 | ATP | dependent on | Niallia circulans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.13 | physiological function | involved in biosynthesis of the aminoglycoside antibiotic butirosin | Niallia circulans |
6.2.1.39 | evolution | BtrJ belongs to the ATP-grasp superfamily | Niallia circulans |
6.2.1.39 | metabolism | butirosin biosynthetic cluster, proceeds via intermediates tethered to a specific acyl carrier protein and gamma-glutamylation of an ACP-derived gamma-aminobutyrate intermediate. The pathway involves the acyl carrier protein BtrI, an ATP-dependent ligase BtrJ, a pyridoxal phosphate-dependent decarboxylase BtrK, and a two-component flavin-dependent monooxygenase system BtrO and BtrV, overview | Niallia circulans |