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Literature summary extracted from

  • Abdelwahab, N.Z.; Crossman, A.T.; Sullivan, L.; Ferguson, M.A.; Urbaniak, M.D.
    Inhibitors incorporating zinc-binding groups target the GlcNAc-PI de-N-acetylase in Trypanosoma brucei, the causative agent of African sleeping sickness (2012), Chem. Biol. Drug Des., 79, 270-278.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.89 expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.89 1,5-anhydro-2-(carboxymethyl)-2-deoxy-D-glucitol
-
 Trypanosoma brucei
3.5.1.89 1,5-anhydro-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
-
 Trypanosoma brucei
3.5.1.89 1,5-anhydro-3-O-benzyl-2-(carboxymethyl)-2-deoxy-D-glucitol
-
 Trypanosoma brucei
3.5.1.89 1,5-anhydro-3-O-benzyl-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
-
 Trypanosoma brucei
3.5.1.89 2-(carboxymethyl)-2-deoxy-D-glucopyranose
-
 Trypanosoma brucei
3.5.1.89 phenyl 2-(carbamoylamino)-2-deoxy-1-thio-beta-D-glucopyranoside
-
 Trypanosoma brucei
3.5.1.89 phenyl 2-(carboxymethyl)-2-deoxy-1-thio-alpha-D-glucopyranoside
-
 Trypanosoma brucei
3.5.1.89 triethylammonium 1R,2R-1-O-[2-C-(carboxymethyl N-hydroxyamide)-2-deoxy-beta-D-glucopyranosyl]-cyclohexanediol 2-(n-octadecylphosphate)
-
 Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.89 Zn2+ a zinc metalloenzyme Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.89 Trypanosoma brucei
-
 variant MITat1.4
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.89 D-GlcNAc-alpha(1->6)-D-myo-inositol-1-octadecyl phosphate + H2O
-
 Trypanosoma brucei D-GlcN-alpha(1->6)-D-myo-inositol-1-octadecyl phosphate + acetate
-
 ?
3.5.1.89 N-acetyl-D-glucosaminylphosphatidylinositol + H2O + H2O
-
 Trypanosoma brucei D-glucosaminylphosphatidylinositol + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.89 deNAc
-
 Trypanosoma brucei
3.5.1.89 GlcNAc-PI de-N-acetylase
-
 Trypanosoma brucei
3.5.1.89 N-acetylglucosamine-phosphatidylinositolde-N-acetylase
-
 Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.89 0.019
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei triethylammonium 1R,2R-1-O-[2-C-(carboxymethyl N-hydroxyamide)-2-deoxy-beta-D-glucopyranosyl]-cyclohexanediol 2-(n-octadecylphosphate)
3.5.1.89 0.1
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei phenyl 2-(carboxymethyl)-2-deoxy-1-thio-alpha-D-glucopyranoside
3.5.1.89 0.29
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 1,5-anhydro-3-O-benzyl-2-(carboxymethyl)-2-deoxy-D-glucitol
3.5.1.89 0.3
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 2-(carboxymethyl)-2-deoxy-D-glucopyranose
3.5.1.89 1.5
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 1,5-anhydro-3-O-benzyl-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
3.5.1.89 10
-
 IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei phenyl 2-(carboxymethyl)-2-deoxy-1-thio-alpha-D-glucopyranoside
3.5.1.89 10
-
 IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 1,5-anhydro-2-(carboxymethyl)-2-deoxy-D-glucitol
3.5.1.89 10
-
 IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 1,5-anhydro-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
3.5.1.89 10
-
 IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei phenyl 2-(carbamoylamino)-2-deoxy-1-thio-beta-D-glucopyranoside