Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, Y.; Zhou, Y.; Ma, Y.; Li, X.
    Design and synthesis of novel cell wall inhibitors of Mycobacterium tuberculosis GlmM and GlmU (2011), Carbohydr. Res., 346, 1714-1720.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.4 expression in Escherichia coli Mycobacterium tuberculosis
2.3.1.157 gene glmU, expression in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.4 2-amino-2,3-dideoxy-3-fluoro-alpha-D-glucopyranosyl phosphate inhibition of acetyltransferase activity. Compound cannot substitute the natural substrate GlcN-1-P. Uncompetitive inhibition, compound interacts with the enzyme–substrate complex. Mycobacterium tuberculosis
2.3.1.157 2-amino-2,3-dideoxy-3-fluoro-alpha-D-glucopyranosyl phosphate
-
 Mycobacterium tuberculosis
2.3.1.157 additional information inhibitor synthesis, detailed overview. No inhibition of GlmU by methyl 2-amino-2-deoxyl-alpha-D-glucopyranoside 6-phosphate, methyl 2-amino-2-deoxyl-beta-D-glucopyranoside 6-phosphate, and 2-azido-2-deoxy-alpha-D-glucopyranosyl phosphate even at high concentrations Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Mycobacterium tuberculosis
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 additional information Mycobacterium tuberculosis GlmU is a bifunctional enzyme converting alpha-D-glucosamine 1-phosphate to N-acetyl-alpha-D-glucosamine 1-phosphate and then catalyzing the formation of UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate and uridine triphosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.4 Mycobacterium tuberculosis
-
 bifunctional glucosamine-1-phosphate acetyltransferase/N-acetylglucosamine-1-phosphate uridyltransferase
-
2.3.1.157 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Mycobacterium tuberculosis CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 additional information GlmU is a bifunctional enzyme converting alpha-D-glucosamine 1-phosphate to N-acetyl-alpha-D-glucosamine 1-phosphate and then catalyzing the formation of UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate and uridine triphosphate Mycobacterium tuberculosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.4 GlmU
-
 Mycobacterium tuberculosis
2.3.1.157 GlmU
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.157 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.157 7.5
-
 assay at Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.4 18.7
-
 2-amino-2,3-dideoxy-3-fluoro-alpha-D-glucopyranosyl phosphate pH 7.5, 37°C Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.4 12.84
-
 pH 7.5, 37°C Mycobacterium tuberculosis 2-amino-2,3-dideoxy-3-fluoro-alpha-D-glucopyranosyl phosphate