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Literature summary extracted from

  • Shih, C.J.; Lai, M.C.
    Differentially expressed genes after hyper- and hypo-salt stress in the halophilic archaeon Methanohalophilus portucalensis (2010), Can. J. Microbiol., 56, 295-307.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.27 DNA and amino acid sequence determination and analysis, sequence comparisons to other enzymes of the methanogenesis pathway Methanohalophilus portucalensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methanohalophilus portucalensis
-
 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methanohalophilus portucalensis FDF1
-
 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.27 Methanohalophilus portucalensis A5JHE4 DSM7471, originally isolated from the solar saltern of Figueria da Foz, Portugal
-
3.5.4.27 Methanohalophilus portucalensis FDF1 A5JHE4 DSM7471, originally isolated from the solar saltern of Figueria da Foz, Portugal
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanohalophilus portucalensis 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O enzyme residue Lys77 binds to the phosphate moiety of substrate moiety H4MPT Methanohalophilus portucalensis 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanohalophilus portucalensis FDF1 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O enzyme residue Lys77 binds to the phosphate moiety of substrate moiety H4MPT Methanohalophilus portucalensis FDF1 5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.27 methenyl-H4MPT cyclohydrolase
-
 Methanohalophilus portucalensis

Expression

EC Number Organism Comment Expression
3.5.4.27 Methanohalophilus portucalensis NaCl downregulates methenyl-H4MPT cyclohydrolase expression down

General Information

EC Number General Information Comment Organism
3.5.4.27 metabolism several of the differentially expressed genes during long-term hyper- or hypo-salt stress are related to the unique energy-acquiring methanogenesis pathway in this organism, including the transmembrane protein MttP, cobalamin biosynthesis protein, methenyl-H4MPT cyclohydrolase and monomethylamine methyltransferase. The methenyl-H4MPT cyclohydrolase participates in the pathway of methyl-group oxidation for transferring methanogenesis substrates into biosynthesis substrates Methanohalophilus portucalensis