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Literature summary extracted from

  • Heuston, E.; Bronner, C.E.; Kull, F.J.; Endow, S.A.
    A kinesin motor in a force-producing conformation (2010), BMC Struct. Biol., 10, 19.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.4 residues H293-K700 for crystallization, GST fusion protein (residues K210-K700) for biochemical and motility assays expressed in Escherichia coli BL21(DE3)pLysS or Rosetta2(DE3)pLysS Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.6.1.4 hanging drops,room temperature, PEG 8000 Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
5.6.1.4 T436S the mutant enzyme permits more rapid nucleotide exchange and moves more slowly along microtubles in gliding assays Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.4 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.4 ion exchange chromatography (SP Sepharose) and ion exchange chromatography (MonoQ) or gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.4 ATP + H2O + a kinesin associated with a microtubule at position n
-
 Drosophila melanogaster ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.4 kinesin-14 Ncd
-
 Drosophila melanogaster