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Literature summary extracted from

  • Caniard, A.; Zerbe, P.; Legrand, S.; Cohade, A.; Valot, N.; Magnard, J.L.; Bohlmann, J.; Legendre, L.
    Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture (2012), BMC Plant Biol., 12, 119.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.3.B31 synthesis synthesis of sclareol by co-expression of producing labda-13-en-8-ol diphosphate as major product and manool synthase in Saccharomyces cerevisiae Salvia sclarea
4.2.3.141 synthesis synthesis of sclareol by co-expression of producing labda-13-en-8-ol diphosphate as major product and manool synthase in Saccharomyces cerevisiae Salvia sclarea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.B31 expression in Escherichia coli and Saccharomyces cerevisiae Salvia sclarea
4.2.3.141 expression in Escherichia coli and Saccharomyces cerevisiae Salvia sclarea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.B31 chloroplast
-
 Salvia sclarea 9507
-
4.2.3.141 chloroplast
-
 Salvia sclarea 9507
-
4.2.3.141 chloroplast enzyme possesses an N-terminal signal sequence that actively targets sclareol synthase to the chloroplast Salvia sclarea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.B31 61000
-
 x * 61000, SDS-PAGE Salvia sclarea
4.2.3.141 61000
-
 x * 61000, SDS-PAGE Salvia sclarea

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.B31 Salvia sclarea K4HYB0 c.f. EC 4.2.3.141
-
4.2.3.141 Salvia sclarea
-
-
-
4.2.3.141 Salvia sclarea K4HYB0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.B31 (9S,10S)-copalyl diphosphate + H2O
-
 Salvia sclarea manool + diphosphate
-
 ?
4.2.3.B31 labda-13-en-8-ol diphosphate + H2O
-
 Salvia sclarea sclareol + diphosphate
-
 ?
4.2.3.B31 additional information no substrate: geranylgeranyl diphosphate Salvia sclarea ?
-
 ?
4.2.3.141 (9S,10S)-copalyl diphosphate + H2O
-
 Salvia sclarea manool + diphosphate
-
 ?
4.2.3.141 labda-13-en-8-ol diphosphate + H2O
-
 Salvia sclarea sclareol + diphosphate
-
 ?
4.2.3.141 additional information in coupled enzyme assays with labda-13-en-8-ol diphosphate synthase, sclareol is the major product with minor amounts of manool, manoyl oxides and epi-manoyl oxide as secondary products Salvia sclarea ?
-
 ?
4.2.3.141 additional information no substrate: geranylgeranyl diphosphate Salvia sclarea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.B31 ? x * 61000, SDS-PAGE Salvia sclarea
4.2.3.141 ? x * 61000, SDS-PAGE Salvia sclarea

Synonyms

EC Number Synonyms Comment Organism
4.2.3.141 sclareol synthase
-
 Salvia sclarea

General Information

EC Number General Information Comment Organism
4.2.3.141 physiological function enzymes labda-13-en-8-ol diphosphate synthase and sclareol synthase are two monofunctional diterpene synthases which, together, produce the diterpenoid specialized metabolite sclareol in a two-step process. Labda-13-en-8-ol diphosphate synthase produces labda-13-en-8-ol diphosphate as major product from geranylgeranyl diphosphate with some minor quantities of its non-hydroxylated analogue, (9 S, 10 S)-copalyl diphosphate. Sclareol synthase then transformes these intermediates into sclareol and manool, respectively Salvia sclarea