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Literature summary extracted from

  • Shang, Y.S.; Zhang, X.E.; Wang, X.D.; Guo, Y.C.; Zhang, Z.P.; Zhou, Y.F.
    Biochemical characterization and mutational improvement of a thermophilic esterase from Sulfolobus solfataricus P2 (2010), Biotechnol. Lett., 32, 1151-1157.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.1 F188A activity with 4-nitrophenyl butyrate as substrate is unchanged as compared to the wild-type value Saccharolobus solfataricus
3.1.1.1 K187A partial loss of activity with 4-nitrophenyl butyrate as substrate Saccharolobus solfataricus
3.1.1.1 R210A catalytic efficiency (kcat/Km) of the mutant enzyme triples over that of wild-type enzyme Saccharolobus solfataricus
3.1.1.1 T58A partial loss of activity with 4-nitrophenyl butyrate as substrate Saccharolobus solfataricus
3.1.1.1 W59A activity with 4-nitrophenyl butyrate as substrate is unchanged as compared to the wild-type value Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 PMSF
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.028
-
 4-nitrophenyl caprylate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.1 0.043
-
 4-nitrophenyl caprylate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus
3.1.1.1 0.63
-
 4-nitrophenyl butyrate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus
3.1.1.1 0.93
-
 4-nitrophenyl butyrate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 58400
-
 x * 58400, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Saccharolobus solfataricus Q97VU1
-
-
3.1.1.1 Saccharolobus solfataricus P2 Q97VU1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O specific activity is less than 10% compared to the activity with caprylate Saccharolobus solfataricus 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O specific activity is less than 10% compared to the activity with caprylate Saccharolobus solfataricus P2 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O specific activity is about 80% compared to the activity with caprylate Saccharolobus solfataricus 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O specific activity is about 80% compared to the activity with caprylate Saccharolobus solfataricus P2 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl caprylate + H2O the enzyme shows the highest specific activity with p-nitrophenyl caprylate Saccharolobus solfataricus 4-nitrophenol + caprylate
-
 ?
3.1.1.1 4-nitrophenyl caprylate + H2O the enzyme shows the highest specific activity with p-nitrophenyl caprylate Saccharolobus solfataricus P2 4-nitrophenol + caprylate
-
 ?
3.1.1.1 4-nitrophenyl decanoate + H2O specific activity is about 50% compared to the activity with caprylate Saccharolobus solfataricus 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl decanoate + H2O specific activity is about 50% compared to the activity with caprylate Saccharolobus solfataricus P2 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl laurate + H2O specific activity is about 10% compared to the activity with caprylate Saccharolobus solfataricus 4-nitrophenol + laurate
-
 ?
3.1.1.1 4-nitrophenyl laurate + H2O specific activity is about 10% compared to the activity with caprylate Saccharolobus solfataricus P2 4-nitrophenol + laurate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 58400, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 SsoPEst
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 80
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 80
-
 1 h, about 50% loss of activity Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 1.7
-
 4-nitrophenyl caprylate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.1 4
-
 4-nitrophenyl butyrate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.1 7.8
-
 4-nitrophenyl caprylate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus
3.1.1.1 10
-
 4-nitrophenyl butyrate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 5.5
-
-
 Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.1 5.1
-
 4-nitrophenyl butyrate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.1 17
-
 4-nitrophenyl butyrate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus
3.1.1.1 63
-
 4-nitrophenyl caprylate pH 7.4, 37°C, wild-type enzyme Saccharolobus solfataricus
3.1.1.1 192
-
 4-nitrophenyl caprylate pH 7.4, 37°C, mutant enzyme R210A Saccharolobus solfataricus