Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Borges, P.; Pacheco, R.; Karmali, A.
    Pseudomonas aeruginosa amidase: aggregation in recombinant Escherichia coli (2011), Biotechnol. J., 6, 888-897.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.4 gene amiE, expression in Escherichia coli strain JM109 using plasmid pKK, production method evaluation and optimization, expression of amidase in soluble form and in aggregates in insoluble fractions, overview Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 (2R,3R)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 (2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 (2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 (2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid 5-phenylpentyl ester potent inhibitor, rapidly cleaved in plasma Pseudomonas aeruginosa
3.5.1.4 (2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 (2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 (2S,3S)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 (R)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 (R)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
 Pseudomonas aeruginosa
3.5.1.4 (R)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 (R,S)-2-oxocyclobutylcarbamic acid benzyl ester weak inhibition Pseudomonas aeruginosa
3.5.1.4 (S)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 (S)-1-methyl-3-(2-oxo-3-oxetanyl)urea
-
 Pseudomonas aeruginosa
3.5.1.4 (S)-2-oxo-3-oxetanylcarbamic acid benzyl ester
-
 Pseudomonas aeruginosa
3.5.1.4 3-(4-phenylbutyl)oxetan-2-one
-
 Pseudomonas aeruginosa
3.5.1.4 6-(benzyloxy)-N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
 Pseudomonas aeruginosa
3.5.1.4 6-methoxy-N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
 Pseudomonas aeruginosa
3.5.1.4 cyclobutylcarbamic acid benzyl ester weak inhibition Pseudomonas aeruginosa
3.5.1.4 ethyl 6-[[(3S)-2-oxooxetan-3-yl]carbamoyl]naphthalene-2-carboxylate
-
 Pseudomonas aeruginosa
3.5.1.4 N-[(3S)-2-oxooxetan-3-yl]biphenyl-4-carboxamide
-
 Pseudomonas aeruginosa
3.5.1.4 N-[(3S)-2-oxooxetan-3-yl]naphthalene-2-carboxamide
-
 Pseudomonas aeruginosa
3.5.1.4 N-[(benzyloxy)carbonyl]-D-serine weak inhibition Pseudomonas aeruginosa
3.5.1.4 N-[(benzyloxy)carbonyl]-L-serine weak inhibition Pseudomonas aeruginosa
3.5.1.4 phenyl 6-[[(3S)-2-oxooxetan-3-yl]carbamoyl]naphthalene-2-carboxylate
-
 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Pseudomonas aeruginosa
-
 gene amiE
-
3.5.1.4 Pseudomonas aeruginosa 8602
-
 gene amiE
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.1.4 solubilization of amidase aggregates in insoluble fractions with up to 1.5 M, of solubilizing agent such as L-arginine or GdnHCl, followed by washing, centrifugation and dilution Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 additional information formation of acetohydroxamic acid using acetamide and hydroxylamine as substrates Pseudomonas aeruginosa ?
-
 ?
3.5.1.4 additional information formation of acetohydroxamic acid using acetamide and hydroxylamine as substrates Pseudomonas aeruginosa 8602 ?
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.4 0.000115
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa N-[(3S)-2-oxooxetan-3-yl]biphenyl-4-carboxamide
3.5.1.4 0.000127
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid 5-phenylpentyl ester
3.5.1.4 0.00019
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (2S,3S)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
3.5.1.4 0.00022
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
3.5.1.4 0.0005
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (R)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
3.5.1.4 0.00058
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (S)-(2-oxo-3-oxetanyl)carbamic acid tert-butyl ester
3.5.1.4 0.0007
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (R)-2-oxo-3-oxetanylcarbamic acid benzyl ester
3.5.1.4 0.001
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (2S,3R)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester
3.5.1.4 0.00296
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (S)-2-oxo-3-oxetanylcarbamic acid benzyl ester
3.5.1.4 0.0049
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (2R,3R)-allo-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
3.5.1.4 0.015
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (S)-1-methyl-3-(2-oxo-3-oxetanyl)urea
3.5.1.4 0.0492
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (R)-1-methyl-3-(2-oxo-3-oxetanyl)urea
3.5.1.4 0.08
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid tert-butyl ester
3.5.1.4 0.1
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa N-[(benzyloxy)carbonyl]-L-serine
3.5.1.4 0.1
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa N-[(benzyloxy)carbonyl]-D-serine
3.5.1.4 0.1
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa cyclobutylcarbamic acid benzyl ester
3.5.1.4 0.1
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (R,S)-2-oxocyclobutylcarbamic acid benzyl ester
3.5.1.4 0.1
-
 pH 5.0, 37°C, recombinant enzyme Pseudomonas aeruginosa (2R,3S)-2-methyl-4-oxo-3-oxetanylcarbamic acid benzyl ester