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Literature summary extracted from

  • De Groeve, M.R.; Remmery, L.; Van Hoorebeke, A.; Stout, J.; Desmet, T.; Savvides, S.N.; Soetaert, W.
    Construction of cellobiose phosphorylase variants with broadened acceptor specificity towards anomerically substituted glucosides (2010), Biotechnol. Bioeng., 107, 413-420.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.20 E649C mutant accepts methyl beta-D-glucoside and ethyl beta-D-glucoside as substrates Cellulomonas uda
2.4.1.20 N156D/N163D/T508I/E649G/N667A mutant with very broad acceptor specificity Cellulomonas uda
2.4.1.20 T508I/E649C/N667A triple mutation broadens acceptor specificity Cellulomonas uda
2.4.1.20 T508I/N667A mutant accepts methyl beta-D-glucoside and ethyl beta-D-glucoside as substrates Cellulomonas uda

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.20 10.8
-
cellobiose mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 17.2
-
D-glucose mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 28.9
-
D-glucose mutant T508I/E649C/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 31.2
-
phenyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 33.7
-
butyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 56.6
-
methyl beta-D-glucoside mutant T508I/E649C/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 79.8
-
methyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 138
-
ethyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 155
-
ethyl beta-D-glucoside mutant T508I/E649C/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 1009
-
methyl alpha-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Cellulomonas uda
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 alpha-D-glucose 1-phosphate + butyl beta-D-glucoside no substrate for wild-type Cellulomonas uda ? + phosphate
-
r
2.4.1.20 alpha-D-glucose 1-phosphate + cellobiose no substrate for wilalpha-D-type Cellulomonas uda ? + phosphate
-
r
2.4.1.20 alpha-D-glucose 1-phosphate + D-glucose
-
Cellulomonas uda ? + phosphate
-
r
2.4.1.20 alpha-D-glucose 1-phosphate + ethyl beta-D-glucoside no substrate for wild-type Cellulomonas uda ? + phosphate
-
r
2.4.1.20 alpha-D-glucose 1-phosphate + methyl alpha-D-glucoside no substrate for wild-type Cellulomonas uda ? + phosphate
-
r
2.4.1.20 alpha-D-glucose 1-phosphate + methyl beta-D-glucoside no substrate for wild-type Cellulomonas uda ? + phosphate
-
r
2.4.1.20 alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside no substrate for wild-type Cellulomonas uda ? + phosphate
-
r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.20 2.6
-
cellobiose mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 4
-
phenyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 5.1
-
ethyl beta-D-glucoside mutant T508I/E649C/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 5.3
-
D-glucose mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 6
-
methyl alpha-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 6.3
-
D-glucose mutant T508I/E649C/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 6.8
-
methyl beta-D-glucoside mutant T508I/E649C/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 7.9
-
methyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 8.7
-
ethyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 9
-
butyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.20 0.006
-
methyl alpha-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 0.031
-
D-glucose mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 0.033
-
ethyl beta-D-glucoside mutant T508I/E649C/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 0.063
-
ethyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 0.099
-
methyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 0.12
-
methyl beta-D-glucoside mutant T508I/E649C/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 0.129
-
phenyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 0.217
-
D-glucose mutant T508I/E649C/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 0.24
-
cellobiose mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda
2.4.1.20 0.266
-
butyl beta-D-glucoside mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C Cellulomonas uda