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Literature summary extracted from

  • Hu, Y.; Peng, N.; Han, W.; Mei, Y.; Chen, Z.; Feng, X.; Liang, Y.X.; She, Q.
    An archaeal protein evolutionarily conserved in prokaryotes is a zinc-dependent metalloprotease (2012), Biosci. Rep., 32, 609-618.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.B29 leupeptin 1 mM, 1.7fold activation Saccharolobus solfataricus
3.4.24.B29 PMSF 5 mM, 1.2fold activation Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B29 overexpression in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.B29 C416G 4% of the activity compared to wild-type enzyme, azocasein as substrate Saccharolobus solfataricus
3.4.24.B29 E229D 11% of the activity compared to wild-type enzyme, azocasein as substrate Saccharolobus solfataricus
3.4.24.B29 H228F 8% of the activity compared to wild-type enzyme, azocasein as substrate Saccharolobus solfataricus
3.4.24.B29 H233Y 14% of the activity compared to wild-type enzyme, azocasein as substrate Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B29 1,10-phenanthroline 10 mM, 90% inhibition Saccharolobus solfataricus
3.4.24.B29 Co2+ 5 mM, 91% inhibition Saccharolobus solfataricus
3.4.24.B29 Cu2+ 5 mM, 37% inhibition Saccharolobus solfataricus
3.4.24.B29 E-64 0.01 mM, 44% inhibition Saccharolobus solfataricus
3.4.24.B29 EDTA 5 mM, complete inhibition Saccharolobus solfataricus
3.4.24.B29 Fe2+ 5 mM, 93% inhibition Saccharolobus solfataricus
3.4.24.B29 Hg2+ 5 mM, 15% inhibition Saccharolobus solfataricus
3.4.24.B29 Mg2+ 5 mM, 97% inhibition Saccharolobus solfataricus
3.4.24.B29 Mn2+ 5 mM, 37% inhibition Saccharolobus solfataricus
3.4.24.B29 N-ethylmaleimide 5 mM, 64% inhibition Saccharolobus solfataricus
3.4.24.B29 Sr2+ 5 mM, 83% inhibition Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B29 Zn2+ 5 mM, stimulates activity up to 160%, contains a zinc-binding motif Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B29 55000
-
 2 * 55000, the protein forms disulfide bond via the Cys416 residue, yielding protein dimer that is the active form of the enzyme, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B29 Saccharolobus solfataricus Q7LXP6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B29
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B29 azocasein + H2O
-
 Saccharolobus solfataricus fragments of azocasein
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.B29 dimer 2 * 55000, the protein forms disulfide bond via the Cys416 residue, yielding protein dimer that is the active form of the enzyme, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B29 Sso0660
-
 Saccharolobus solfataricus
3.4.24.B29 TldD
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.B29 55 60
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.B29 45 85 45°C: about 85% of maximal activity, 85°C: about 60% of maximal activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.B29 7
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.B29 6 9 6: more than 75% of maximal activity, pH 9: about 80% of maximal activity Saccharolobus solfataricus