EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.17.B4 | overexpression in Escherichia coli | Thermococcus onnurineus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.17.B4 | Cu2+ | 0.2 mM, about 90% loss of activity, the inhibitory effect is not overcome by the presence of Co2+ | Thermococcus onnurineus | |
3.4.17.B4 | EDTA | 1 mM, complete inactivation | Thermococcus onnurineus | |
3.4.17.B4 | Fe2+ | 0.2 mM, about 70% loss of activity, the inhibitory effect is not overcome by the presence of Co2+ | Thermococcus onnurineus | |
3.4.17.B4 | Ni2+ | 0.2 mM, about 75% loss of activity, the inhibitory effect is not overcome by the presence of Co2+ | Thermococcus onnurineus | |
3.4.17.B4 | Zn2+ | 0.2 mM, about 90% loss of activity, the inhibitory effect is not overcome by the presence of Co2+ | Thermococcus onnurineus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.17.B4 | 1.4 | - |
N-benzyloxycarbonyl-Ala-Arg | pH 6.5, 70-80°C | Thermococcus onnurineus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.17.B4 | Co2+ | 0.2 mM, stimulates activity, can not be replaced by other divalent metal ions, no activation by Zn2+ | Thermococcus onnurineus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.17.B4 | 61000 | - |
2 * 61000, SDS-PAGE | Thermococcus onnurineus |
3.4.17.B4 | 135000 | - |
gel filtration | Thermococcus onnurineus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.4.17.B4 | Glycerol | 12.5%, does not protect from inactivation at either temperature | Thermococcus onnurineus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.17.B4 | Thermococcus onnurineus | Q2QC89 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.17.B4 | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + H2O | - |
Thermococcus onnurineus | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro + L-phenylalanine | - |
? | |
3.4.17.B4 | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His + H2O | - |
Thermococcus onnurineus | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + L-histidine | - |
? | |
3.4.17.B4 | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O | - |
Thermococcus onnurineus | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His + L-leucine | - |
? | |
3.4.17.B4 | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu + H2O | - |
Thermococcus onnurineus | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + L-leucine | - |
? | |
3.4.17.B4 | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val + H2O | - |
Thermococcus onnurineus | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu + L-valine | - |
? | |
3.4.17.B4 | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr + H2O | - |
Thermococcus onnurineus | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val + L-tyrosine | - |
? | |
3.4.17.B4 | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O | release of up to seven residues from the C-terminus | Thermococcus onnurineus | Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr + L-serine | - |
? | |
3.4.17.B4 | additional information | no activity with N-benzyloxycarbonyl-Ala-Asp, N-benzyloxycarbonyl-Ala-Asn, N-benzyloxycarbonyl-Ala-Pro, N-benzyloxycarbonyl-Ala-His | Thermococcus onnurineus | ? | - |
? | |
3.4.17.B4 | N-benzyloxycarbonyl-Ala-Ala + H2O | - |
Thermococcus onnurineus | N-benzyloxycarbonyl-Ala + L-alanine | - |
? | |
3.4.17.B4 | N-benzyloxycarbonyl-Ala-Arg + H2O | compared to the activity of Pyrococcus furiosus carboxypeptidase the catalytic efficiency is of Thermococcus onnurineus carboxypeptidase is 80-fold lower | Thermococcus onnurineus | N-benzyloxycarbonyl-Ala + L-arginine | - |
? | |
3.4.17.B4 | N-benzyloxycarbonyl-Ala-Leu + H2O | - |
Thermococcus onnurineus | N-benzyloxycarbonyl-Ala + L-leucine | - |
? | |
3.4.17.B4 | N-benzyloxycarbonyl-Ala-Met + H2O | - |
Thermococcus onnurineus | N-benzyloxycarbonyl-Ala + L-methionine | - |
? | |
3.4.17.B4 | N-benzyloxycarbonyl-Ala-Tyr + H2O | - |
Thermococcus onnurineus | N-benzyloxycarbonyl-Ala + L-tyrosine | - |
? | |
3.4.17.B4 | N-benzyloxycarbonyl-Ala-Val + H2O | - |
Thermococcus onnurineus | N-benzyloxycarbonyl-Ala + L-valine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.17.B4 | dimer | 2 * 61000, SDS-PAGE | Thermococcus onnurineus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.17.B4 | TNA1_CP | - |
Thermococcus onnurineus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.17.B4 | 70 | 80 | substrate: N-benzyloxycarbonyl-Ala-Arg | Thermococcus onnurineus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.17.B4 | 50 | 90 | 50°C: about 50% of maximal activity, 90°C: about 60% of maximal activity | Thermococcus onnurineus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.17.B4 | 80 | - |
pH 6.5, 0.2 mM Co2+, t1/2: 84 min, t1/2 in absence of Co2+ is 7.4 min | Thermococcus onnurineus |
3.4.17.B4 | 90 | - |
pH 6.5, 0.2 mM Co2+, t1/2: 8 min, t1/2 in absence of Co2+ is 5 min | Thermococcus onnurineus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.17.B4 | 11.6 | - |
N-benzyloxycarbonyl-Ala-Arg | pH 6.5, 70-80°C | Thermococcus onnurineus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.17.B4 | 6.5 | - |
substrate: N-benzyloxycarbonyl-Ala-Arg | Thermococcus onnurineus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.17.B4 | 5.3 | 7.5 | pH 5.3: about 80% of maximal activity, pH 7.5: about 45% of maximal activity | Thermococcus onnurineus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.17.B4 | 8.3 | - |
N-benzyloxycarbonyl-Ala-Arg | pH 6.5, 70-80°C | Thermococcus onnurineus |