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Literature summary extracted from

  • Tamegai, H.; Nango, E.; Koike-Takeshita, A.; Kudo, F.; Kakinuma, K.
    Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans (2002), Biosci. Biotechnol. Biochem., 66, 1538-1545.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.124 genes btrC and btrC2, co-overexpression in Escherichia coli strain JM109 Niallia circulans

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.124 additional information disruption of gene btrC2 by insertion of a tetracycline resistance cassette Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.124 0.017
-
 D-glucose 6-phosphate pH 7.7, 35°C, native enzyme Niallia circulans
4.2.3.124 0.23
-
 D-glucose 6-phosphate pH 7.7, 35°C, recombinant wild-type enzyme, BtrC Niallia circulans
4.2.3.124 0.42
-
 D-glucose 6-phosphate pH 7.7, 35°C, recombinant wild-type enzyme, BtrC + BtrC2 Niallia circulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.124 Co2+
-
 Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.124 D-glucose 6-phosphate Niallia circulans
-
 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
4.2.3.124 D-glucose 6-phosphate Niallia circulans SANK72073
-
 2-deoxy-L-scyllo-inosose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.124 Niallia circulans Q9S5E2 genes btrC and btrC2, the latter encoding the 20 kDa enzyme subunit
-
4.2.3.124 Niallia circulans SANK72073 Q9S5E2 genes btrC and btrC2, the latter encoding the 20 kDa enzyme subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.124 recombinant BtrC2 from Escherichia coli strain JM109 by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration Niallia circulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.124 additional information a butirosin producer culture Niallia circulans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.124 D-glucose 6-phosphate
-
 Niallia circulans 2-deoxy-L-scyllo-inosose + phosphate
-
 ?
4.2.3.124 D-glucose 6-phosphate
-
 Niallia circulans SANK72073 2-deoxy-L-scyllo-inosose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.124 BtrC2
-
 Niallia circulans
4.2.3.124 DOI synthase
-
 Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.124 35
-
 assay at Niallia circulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.124 0.073
-
 D-glucose 6-phosphate pH 7.7, 35°C, native enzyme Niallia circulans
4.2.3.124 0.44
-
 D-glucose 6-phosphate pH 7.7, 35°C, recombinant wild-type enzyme, BtrC + BtrC2 Niallia circulans
4.2.3.124 1
-
 D-glucose 6-phosphate pH 7.7, 35°C, recombinant wild-type enzyme, BtrC Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.124 7.7
-
 assay at Niallia circulans

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.124 NAD+
-
 Niallia circulans

General Information

EC Number General Information Comment Organism
4.2.3.124 malfunction disruption of gene btrC2 reduces the growth rate and antibiotics production, the growth rate is restored by addition of NH4Cl, both by addition of yeast extract Niallia circulans
4.2.3.124 metabolism key enzyme in the biosynthesis of 2-deoxytstreptamine. The function od BtrC2 in biosynthesis of butirosin is indirect. BtrC2 is involved in secondary as well as primary metabolism probably playing a role in stabilizing and regulating DOI synthase Niallia circulans