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Literature summary extracted from

  • Thayyullathil, F.; Chathoth, S.; Hago, A.; Patel, M.; Szulc, Z.M.; Hannun, Y.; Galadari, S.
    Purification and characterization of a second type of neutral ceramidase from rat brain: a second more hydrophobic form of rat brain ceramidase (2011), Biochim. Biophys. Acta, 1811, 242-252.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 oxidized glutathione activity is inhibited almost totally at 20 mM Rattus norvegicus
3.5.1.23 reduced glutathione
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 2-mercaptoethanol more than 50% inhibition at 100 mM Rattus norvegicus
3.5.1.23 beta-D-octyl glucoside almost complete inhibition at 1.5% (v/v) Rattus norvegicus
3.5.1.23 Cu2+ about 80% inhibition at 10 mM Rattus norvegicus
3.5.1.23 dihydrosphingosine inhibits enzyme activity by up to 40% at 0.25 mM Rattus norvegicus
3.5.1.23 dithiothreitol 80% inhibition at 100 mM Rattus norvegicus
3.5.1.23 EDTA about 60% residual activity at 1 mM Rattus norvegicus
3.5.1.23 Fe3+ almost complete inhibition at 10 mM Rattus norvegicus
3.5.1.23 Hg2+ complete inhibition at 1 mM Rattus norvegicus
3.5.1.23 additional information GMP, GDT, GTP, TDP, TTP, UDP, and UTP, ADP and AMP have no significant effects on the enzyme activity. S-1-P has no inhibitory effect Rattus norvegicus
3.5.1.23 TMP more than 95% inhibition at 12 mM Rattus norvegicus
3.5.1.23 UMP more than 95% inhibition at 12 mM Rattus norvegicus
3.5.1.23 Zn2+
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 Ca2+ maximum stimulation at 5 mM Ca2+ Rattus norvegicus
3.5.1.23 additional information the enzyme is not affected by Li+, Na+, Mn2+ and Mg2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 95000
-
 x * 95000, deglycosylated protein, SDS-PAGE Rattus norvegicus
3.5.1.23 110000
-
 x * 110000, glycosylated protein, SDS-PAGE Rattus norvegicus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.1.23 Triton X-100 the optimum concentration is 0.9-1.2 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.23 glycoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 Q-Sepharose column chromatography, Superdex 200 gel filtration, heparin-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Mono Q column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 1.1
-
 non-purified enzyme, at pH 7.5 and 37°C Rattus norvegicus
3.5.1.23 16720
-
 after 15134fold purification, at pH 7.5 and 37°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 dihydro-N-myristoyl-D-erythro-sphingosine + H2O 55.9% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus dihydro-D-erythro-sphingosine + myristate
-
 ?
3.5.1.23 dihydro-N-palmitoyl-D-erythro-sphingosine + H2O 4.5% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus dihydro-D-erythro-sphingosine + palmitate
-
 ?
3.5.1.23 additional information long chain dihydroceramide dihydro-hexanoyl-D-erythro-sphingosine is resistant to the enzyme Rattus norvegicus ?
-
 ?
3.5.1.23 N-hexanoyl-D-erythro-sphingosine + H2O 100.3% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + hexanoate
-
 ?
3.5.1.23 N-lauroyl-D-erythro-sphingosine + H2O most efficient substrate, 293.2% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + dodecanoate
-
 r
3.5.1.23 N-lignoceroyl-D-erythro-sphingosine + H2O 25.1% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + tetracosanoate
-
 ?
3.5.1.23 N-myristoyl-D-erythro-sphingosine + H2O 212.2% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + myristate
-
 ?
3.5.1.23 N-palmitoyl-D-erythro-sphingosine + H2O 100% hydrolysis Rattus norvegicus sphingosine + palmitate
-
 r
3.5.1.23 N-stearoyl-D-erythro-sphingosine + H2O 98.5% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine Rattus norvegicus sphingosine + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 110000, glycosylated protein, SDS-PAGE Rattus norvegicus
3.5.1.23 ? x * 95000, deglycosylated protein, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 CDase II
-
 Rattus norvegicus
3.5.1.23 neutral ceramidase
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 7.5
-
-
 Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.23 Rattus norvegicus isoelectric focusing
-
 7.2

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.23 1
-
 in 50 mM Tris (pH 7.5) containing 0.3% (v/v) Triton X-100, at 37°C Rattus norvegicus Fe3+
3.5.1.23 5
-
 in 50 mM Tris (pH 7.5) containing 0.3% (v/v) Triton X-100, at 37°C Rattus norvegicus Zn2+
3.5.1.23 20
-
 in 50 mM Tris (pH 7.5) containing 0.3% (v/v) Triton X-100, at 37°C Rattus norvegicus EDTA