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Literature summary extracted from
- Interaction of fosfomycin with the glycerol 3-phosphate transporter of Escherichia coli (2011), Biochim. Biophys. Acta, 1810, 1323-1329.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.6.2.10 | expression of His-tagged enzyme in Escherichia coli strain SH116 | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.10 | 4-chloromercuribenzoate | 40.5% inhibition at 0.1 mM | Escherichia coli |  |
| 7.6.2.10 | bathophenanthroline | 96.2% inhibition at 10 mM | Escherichia coli | |
| 7.6.2.10 | fosfomycin | fosfomycin, externally added to the proteoliposomes, poorly inhibits the glycerol-3-phosphate/glycerol-3-phosphate antiport catalyzed by the reconstituted transporter, completely competitive, interaction of fosfomycin with the recombinant and purified GlpT of Escherichia coli reconstituted in liposomes, overview | Escherichia coli | |
| 7.6.2.10 | Mersalyl | 71.1% inhibition at 0.1 mM | Escherichia coli | |
| 7.6.2.10 | N-ethylmaleimide | 1.5% inhibition at 1 mM | Escherichia coli | |
| 7.6.2.10 | Phthalic acid | 48.8% inhibition at 10 mM | Escherichia coli | |
| 7.6.2.10 | pyridoxal 5'-phosphate | 98.5% inhibition at 10 mM | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.6.2.10 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.6.2.10 | recombinant His-tagged enzyme 55fold from Escherichia coli strain SH116 by nickel affinity chromatography | Escherichia coli |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 7.6.2.10 | reconstitution of the recombinant His-tagged GlpT into liposomes | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.10 | additional information | the enzyme performs G3P/G3P exchange, it shows virtually poor exchange activity using phosphoenolpyruvate, fosfomycin and sulphate, and no activity with glutamate, glutamine, aspartate and ATP. Highest activity in the presence of internal glycerol-3-phosphate and phosphate | Escherichia coli | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.6.2.10 | GlpT | - |
Escherichia coli |
| 7.6.2.10 | glycerol 3-phosphate transporter | - |
Escherichia coli |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 7.6.2.10 | 6.4 | - |
externally added to the proteoliposomes with reconstituted transporter, pH and temperature not specified in the publication | Escherichia coli | fosfomycin | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.6.2.10 | additional information | fosfomycin is widely used to treat urinary tract and pediatric gastrointestinal infections of bacteria. It is supposed that this antibiotic enters cells via two transport systems, including the bacterial glycerol-3-phosphate transporter, GlpT. Impaired function of GlpT is one mechanism for fosfomycin resistance. Interaction of fosfomycin with the recombinant and purified GlpT of Escherichia coli reconstituted in liposomes, overview | Escherichia coli |
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