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Literature summary extracted from

  • Kan, S.C.; Liu, J.S.; Hu, H.Y.; Chang, C.M.; Lin, W.D.; Wang, W.C.; Hsu, W.H.
    Biochemical characterization of two thymidylate synthases in Corynebacterium glutamicum NCHU 87078 (2010), Biochim. Biophys. Acta, 1804, 1751-1759.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 expressed in Escherichia coli NovaBlue cells Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 29100
-
 x * 29100, SDS-PAGE Corynebacterium glutamicum
2.1.1.45 29400
-
 x * 29400, calculated from amino acid sequence Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Corynebacterium glutamicum
-
-
-
2.1.1.45 Corynebacterium glutamicum NCHU 87078
-
-
-
2.1.1.148 no activity in Corynebacterium glutamicum strain NCHU 87078
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 nickel-chelate column chromatography, gel filtration Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 414
-
 purified enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 37°C Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Corynebacterium glutamicum dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Corynebacterium glutamicum NCHU 87078 dihydrofolate + dTMP
-
 ?
2.1.1.45 additional information thymidylate synthase form ThyX has no activity in Corynebacterium glutamicum Corynebacterium glutamicum ?
-
 ?
2.1.1.45 additional information thymidylate synthase form ThyX has no activity in Corynebacterium glutamicum Corynebacterium glutamicum NCHU 87078 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 ? x * 29100, SDS-PAGE Corynebacterium glutamicum
2.1.1.45 ? x * 29400, calculated from amino acid sequence Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.45 ThyA
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.45 additional information the enzyme is FAD-independent Corynebacterium glutamicum
2.1.1.148 FAD
-
 no activity in Corynebacterium glutamicum strain NCHU 87078

General Information

EC Number General Information Comment Organism
2.1.1.45 metabolism ThyA is the sole catalyst for thymidylate biosynthesis in Corynebacterium glutamicum Corynebacterium glutamicum