Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Marapakala, K.; Qin, J.; Rosen, B.P.
    Identification of catalytic residues in the As(III) S-adenosylmethionine methyltransferase (2012), Biochemistry, 51, 944-951.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.137 glutathione As(III) SAM methyltransferase conducts three successive rounds of oxidative methylation, with the glutathione conjugates serving as metalloid donors to the enzyme Cyanidioschyzon sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.137 expressed in Escherichia coli BL21(DE3) cells Cyanidioschyzon sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.137 C174A the mutation leads to loss of As(III) methylation Cyanidioschyzon sp.
2.1.1.137 C224A the mutation leads to loss of As(III) methylation Cyanidioschyzon sp.
2.1.1.137 C72A the mutation leads to loss of As(III) methylation, but still shows trivalent methylarsenate methylation Cyanidioschyzon sp.
2.1.1.137 Y70W the mutant exhibits metalloid binding Cyanidioschyzon sp.

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.137 Cyanidioschyzon sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.137 Ni-NTA column chromatography Cyanidioschyzon sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.137 S-adenosyl-L-methionine + arsenite the first product, methylarsonate, undergoes a second round of methylation faster than it dissociates from the enzyme. The second product, dimethylarsinate, dissociates faster than it undergoes the third round of methylation to the third and final product, trimethylarsine Cyanidioschyzon sp. S-adenosyl-L-homocysteine + methylarsonate + dimethylarsinate + trimethylarsine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.137 ArsM
-
 Cyanidioschyzon sp.
2.1.1.137 As(III) S-adenosylmethionine methyltransferase
-
 Cyanidioschyzon sp.
2.1.1.137 As(III) SAM methyltransferase
-
 Cyanidioschyzon sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.137 60 70
-
 Cyanidioschyzon sp.