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Literature summary extracted from

  • Koch, B.; Kolenko, P.; Buchholz, M.; Ruiz Carrillo, D.; Parthier, C.; Wermann, M.; Rahfeld, J.U.; Reuter, G.; Schilling, S.; Stubbs, M.T.; Demuth, H.U.
    Crystal structures of glutaminyl cyclases (QCs) from Drosophila melanogaster reveal active site conservation between insect and mammalian QCs (2012), Biochemistry, 51, 7383-7392.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.5 expressed in Escherichia coli M15 cells (isoform isoDromeQC) Drosophila melanogaster
2.3.2.5 expressed in Pichia pstoris strain X33 (isoform DromeQC) Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.5 hanging drop vapor diffusion method, using 10% (w/v) PEG 35000 and 100 mM Tris (pH 8.5) for isoform DromeQC Drosophila melanogaster
2.3.2.5 hanging drop vapor diffusion method, using 200 mM MnCl2, 30% (w/v) PEG 4000, and 100 mM Tris (pH 8.5) for isoform isoDromeQC Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.5 C113A/C136A mutant of isoform DromeQC lacking a disulfide bond, without influence on the stability of the enzyme Drosophila melanogaster
2.3.2.5 C136A/C158A mutant of isoform isoDromeQC lacking a disulfide bond, without influence on the stability of the enzyme Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.5 1-Methylimidazole
-
 Drosophila melanogaster
2.3.2.5 benzimidazole
-
 Drosophila melanogaster
2.3.2.5 benzylimidazole
-
 Drosophila melanogaster
2.3.2.5 imidazole
-
 Drosophila melanogaster
2.3.2.5 Nomega-acetylhistamine
-
 Drosophila melanogaster
2.3.2.5 PBD150 potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea; potent competitive inhibitor, i.e. 1-(3,4-dimethoxyphenyl)-3-(3-imidazol-1-ylpropyl)thiourea Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.5 extracellular isoform DromeQC Drosophila melanogaster
-
-
2.3.2.5 mitochondrion isoform isoDromeQC Drosophila melanogaster 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.5 Zn2+ contains zinc Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.5 39SrpL21 protein Drosophila melanogaster
-
 ?
-
 ?
2.3.2.5 Acp33A protein Drosophila melanogaster
-
 ?
-
 ?
2.3.2.5 ALDH2 protein Drosophila melanogaster
-
 ?
-
 ?
2.3.2.5 DIM-10 protein Drosophila melanogaster
-
 ?
-
 ?
2.3.2.5 MCCB protein Drosophila melanogaster
-
 ?
-
 ?
2.3.2.5 NPLP4 protein Drosophila melanogaster
-
 ?
-
 ?
2.3.2.5 rho-7 protein Drosophila melanogaster
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.5 Drosophila melanogaster Q86PD7 isoform isoDromeQC
-
2.3.2.5 Drosophila melanogaster Q9VRQ9 isoform DromeQC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.5 39SrpL21 protein
-
 Drosophila melanogaster ?
-
 ?
2.3.2.5 Acp33A protein
-
 Drosophila melanogaster ?
-
 ?
2.3.2.5 ALDH2 protein
-
 Drosophila melanogaster ?
-
 ?
2.3.2.5 DIM-10 protein
-
 Drosophila melanogaster ?
-
 ?
2.3.2.5 L-Gln-2-naphthylamide
-
 Drosophila melanogaster 5-oxoprolyl-2-naphthylamide + NH3
-
 ?
2.3.2.5 L-Gln-7-amido-4-methylcoumarin
-
 Drosophila melanogaster 5-oxoprolyl-7-amido-4-methylcoumarin + NH3
-
 ?
2.3.2.5 L-Gln-Gly
-
 Drosophila melanogaster 5-oxoprolylglycine + NH3
-
 ?
2.3.2.5 L-Gln-Gly-L-Pro
-
 Drosophila melanogaster 5-oxoprolyl-Gly-L-Pro + NH3
-
 ?
2.3.2.5 L-Gln-L-Lys-L-Arg-L-Leu
-
 Drosophila melanogaster 5-oxoprolyl-L-Lys-L-Arg-L-Leu + NH3
-
 ?
2.3.2.5 L-Gln-L-Phe-L-Ala
-
 Drosophila melanogaster 5-oxoprolyl-L-Phe-L-Ala + NH3
-
 ?
2.3.2.5 MCCB protein
-
 Drosophila melanogaster ?
-
 ?
2.3.2.5 NPLP4 protein
-
 Drosophila melanogaster ?
-
 ?
2.3.2.5 rho-7 protein
-
 Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.5 DromeQC isoform Drosophila melanogaster
2.3.2.5 glutaminyl cyclase
-
 Drosophila melanogaster
2.3.2.5 isoDromeQC isoform Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.5 7.3
-
 isoform DromeQC Drosophila melanogaster
2.3.2.5 7.5 8 isoform isoDromeQC Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.2.5 0.0001013
-
 PBD150 isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 0.0001093
-
 PBD150 isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 0.0003706
-
 PBD150 isoform isoDromeQC, at 30°C in 50 mM MES (pH 6.0) Drosophila melanogaster
2.3.2.5 0.000944
-
 PBD150 isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 0.00248
-
 PBD150 isoform DromeQC, at 30°C in 50 mM MES (pH 6.0) Drosophila melanogaster
2.3.2.5 0.003139
-
 PBD150 isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.2.5 4.2
-
 NPLP4 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 4.6
-
 NPLP4 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 6
-
 NPLP4 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 7.4
-
 NPLP4 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 7.8
-
 ALDH2 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 8.3
-
 ALDH2 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 13.9
-
 DIM-10 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 15.9
-
 39SrpL21 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 17.5
-
 DIM-10 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 20.1
-
 NPLP4 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 20.2
-
 39SrpL21 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 20.9
-
 NPLP4 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 20.9
-
 39SrpL21 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 21.1
-
 39SrpL21 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 21.5
-
 NPLP4 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 21.9
-
 39SrpL21 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 22
-
 MCCB protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 22.8
-
 MCCB protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 23.8
-
 NPLP4 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 25
-
 MCCB protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 25.5
-
 MCCB protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 25.9
-
 DIM-10 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 26.1
-
 MCCB protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 26.2
-
 rho-7 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 26.2
-
 ALDH2 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 27.6
-
 39SrpL21 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 28.9
-
 MCCB protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 30.2
-
 rho-7 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 31.2
-
 DIM-10 protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 31.2
-
 DIM-10 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 32
-
 ALDH2 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 32.3
-
 MCCB protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 33.2
-
 MCCB protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 33.9
-
 ALDH2 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 35
-
 Acp33A protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 36
-
 Acp33A protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 36.9
-
 Acp33A protein isoform DromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 38
-
 Acp33A protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 40.9
-
 ALDH2 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 40.9
-
 rho-7 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 43.3
-
 DIM-10 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 43.4
-
 rho-7 protein isoform isoDromeQC, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 45.3
-
 39SrpL21 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 49.9
-
 39SrpL21 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 50.2
-
 rho-7 protein isoform isoDromeQC mutant C136A/C158A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 50.7
-
 DIM-10 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 53.3
-
 rho-7 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 55
-
 rho-7 protein isoform isoDromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 59.3
-
 DIM-10 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM Tris (pH 8.0) Drosophila melanogaster
2.3.2.5 69
-
 ALDH2 protein isoform DromeQC, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 73.1
-
 ALDH2 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster
2.3.2.5 88
-
 rho-7 protein isoform DromeQC mutant C113A/C136A, at 30°C in 50 mM MOPS (pH 7.0) Drosophila melanogaster