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Literature summary extracted from

  • Fan, F.; Williams, H.J.; Boyer, J.G.; Graham, T.L.; Zhao, H.; Lehr, R.; Qi, H.; Schwartz, B.; Raushel, F.M.; Meek, T.D.
    On the catalytic mechanism of human ATP citrate lyase (2012), Biochemistry, 51, 5198-5211.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.8 expressed in Sf9 cells using a a baculovirus expression system Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.8 H760A the turnover number of the mutant is similar to that of wild type in the absence of citrate and CoA, yet significantly less than that of wild type enzyme in the presence of both citrate and CoA Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.8 diethyldicarbonate the addition of 0.5 mM ATP in the preincubation reaction mixture provided complete protection of enzyme activity from inactivation by diethyldicarbonate Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.8 Mg2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.8 110000
-
 4 * 110000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.8 Superose 12 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.8 ATP + citrate + CoA
-
 Homo sapiens ADP + phosphate + acetyl-CoA + oxaloacetate
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.3.8 homotetramer 4 * 110000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.3.8 ACL
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.3.8 19
-
 ATP wild type enzyme, average value, in 50 mM Tris, 10 mM MgCl2, and 2 mM dithiothreitol (pH 8.0), at 22°C Homo sapiens
2.3.3.8 230
-
 citrate wild type enzyme, average value, in 50 mM Tris, 10 mM MgCl2, and 2 mM dithiothreitol (pH 8.0), at 22°C Homo sapiens
2.3.3.8 240
-
 CoA wild type enzyme, average value, in 50 mM Tris, 10 mM MgCl2, and 2 mM dithiothreitol (pH 8.0), at 22°C Homo sapiens