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Literature summary extracted from

  • Casey, A.K.; Baugh, J.; Frantom, P.A.
    The slow-onset nature of allosteric inhibition in alpha-isopropylmalate synthase from Mycobacterium tuberculosis is mediated by a flexible loop (2012), Biochemistry, 51, 4773-4775.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.13 D563A the enzyme is more sensitive to L-leucine inhibition compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 D563N the enzyme is more sensitive to L-leucine inhibition compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 D564A the enzyme is less sensitive to L-leucine inhibition compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 H540A the enzyme is less sensitive to L-leucine inhibition compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 M559A the enzyme is less sensitive to L-leucine inhibition compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 Q566A the enzyme is more sensitive to L-leucine inhibition compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 S560A inactive Mycobacterium tuberculosis
2.3.3.13 S560W the mutation does not affect the activity of the enzyme, but the enzyme is less sensitive to L-leucine inhibition compared to the wild type enzyme Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.13 L-isoleucine
-
Mycobacterium tuberculosis
2.3.3.13 L-leucine slow-onset, allosteric inhibition by L-leucine Mycobacterium tuberculosis
2.3.3.13 L-methionine
-
Mycobacterium tuberculosis
2.3.3.13 L-norleucine micromolar inhibitor Mycobacterium tuberculosis
2.3.3.13 L-norvaline micromolar inhibitor Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.13 additional information the enzyme requires a divalent metal for activity and is activated by monovalent cations Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Mycobacterium tuberculosis 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?

Subunits

EC Number Subunits Comment Organism
2.3.3.13 dimer
-
Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.3.13 alpha-isopropylmalate synthase I
-
Mycobacterium tuberculosis
2.3.3.13 IPMS
-
Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.13 0.0023
-
L-leucine mutant enzyme D563N, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.3.3.13 0.003
-
L-leucine mutant enzyme D563A, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.3.3.13 0.0077
-
L-leucine mutant enzyme Q566A, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.3.3.13 0.012
-
L-leucine wild type enzyme, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.3.3.13 0.023
-
L-leucine mutant enzyme H540A, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.3.3.13 0.024
-
L-leucine mutant enzyme D564A, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.3.3.13 0.051
-
L-leucine mutant enzyme S560W, pH and temperature not specified in the publication Mycobacterium tuberculosis
2.3.3.13 0.155
-
L-leucine mutant enzyme M559A, pH and temperature not specified in the publication Mycobacterium tuberculosis