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Literature summary extracted from

  • Joo, S.H.; Chung, H.S.; Raetz, C.R.; Garrett, T.A.
    Activity and crystal structure of Arabidopsis thaliana UDP-N-acetylglucosamine acyltransferase (2012), Biochemistry, 51, 4322-4330.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.129 gene lpxA, excluding the region encoding the predicted signal peptide (residues 2-32), expression of the plant enzyme in Escherichia coli strain C41(DE3). Functional expression of AtlpxA in and complementation of the constructed lpxA Escherichia coli knockout mutant strains Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.129 purified recombinant LpxA, sitting drop vapor diffusion method, mixing of AtLpxA at 18 mg/mL in 10 mM potassium phosphate buffer, pH 7.0, 200 mM KCl, and 20% glycerol, in a 1:1 ratio with precipitant solution containing 0.5 M ammonium sulfate, 0.1 M sodium citrate, pH 5.6, and 1.0 M lithium sulfate monohydrate, equilibration at 20°C, 1-2 days, X-ray diffraction structure determination and analysis at 2.1 A resolution Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.129 additional information Arabidopsis thaliana lpxA complements an Escherichia coli mutant lacking the chromosomal lpxA and promotes the synthesis of lipid A in vivo similar to the lipid A produced in the presence of Escherichia coli lpxA Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.129 an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine Arabidopsis thaliana
-
 an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.129 Arabidopsis thaliana Q9SU91
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.129 recombinant LpxA, excluding the region encoding the predicted signal peptide (residues 2-32), from Escherichia coli strain C41(DE3) by Reactive Green 19 affinity chromatography and gel filtration to homogeneity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.129 an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Arabidopsis thaliana an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 r
2.3.1.129 an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine specificity for (R)-3-hydroxymyristoyl-ACP over other chain lengths in vitro Arabidopsis thaliana an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.129 LpxA
-
 Arabidopsis thaliana
2.3.1.129 UDP-N-acetylglucosamine acyltransferase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.129 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.129 7.4
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.3.1.129 evolution the overall structure of AtLpxA is very similar to that of Escherichia coli LpxA, EcLpxA, with an alpha-helical-rich C-terminus and characteristic N-terminal left-handed parallel beta-helix. All key catalytic and chain length determining residues of EcLpxA are conserved in AtLpxA,but AtLpxA has an additional coil and loop added to the N-terminal left-handed parallel beta-helix not seen in EcLpxA Arabidopsis thaliana
2.3.1.129 additional information active site structure of AtLpxA, overview Arabidopsis thaliana