Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hobbs, M.E.; Malashkevich, V.; Williams, H.J.; Xu, C.; Sauder, J.M.; Burley, S.K.; Almo, S.C.; Raushel, F.M.
    Structure and catalytic mechanism of LigI: Insight into the amidohydrolase enzymes of cog3618 and lignin degradation (2012), Biochemistry, 51, 3497-3507.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.57 His-tagged version expressed in Escherichia coli BL21(DE3) Sphingomonas paucimobilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.57 sitting drop vapor diffusion method Sphingomonas paucimobilis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.57 D248A prototypical metal-coordinating residue in the amidohydrolase superfamily Sphingomonas paucimobilis
3.1.1.57 D248N prototypical metal-coordinating residue in the amidohydrolase superfamily Sphingomonas paucimobilis
3.1.1.57 H180A prototypical metal-coordinating residue in the amidohydrolase superfamily Sphingomonas paucimobilis
3.1.1.57 H180C prototypical metal-coordinating residue in the amidohydrolase superfamily Sphingomonas paucimobilis
3.1.1.57 H31N prototypical metal-coordinating residue in the amidohydrolase superfamily Sphingomonas paucimobilis
3.1.1.57 H33N prototypical metal-coordinating residue in the amidohydrolase superfamily Sphingomonas paucimobilis
3.1.1.57 R124M active site residue Sphingomonas paucimobilis
3.1.1.57 R130M active site residue Sphingomonas paucimobilis
3.1.1.57 R183M active site residue Sphingomonas paucimobilis
3.1.1.57 R217M active site residue Sphingomonas paucimobilis
3.1.1.57 Y156F active site residue Sphingomonas paucimobilis
3.1.1.57 Y49F active site residue Sphingomonas paucimobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.57 5-hydroxyisophthalic acid
-
 Sphingomonas paucimobilis
3.1.1.57 pyridine-2,4-dicarboxylic acid
-
 Sphingomonas paucimobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.57 0.041
-
 2-Pyrone-4,6-dicarboxylate H180C mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.048
-
 2-Pyrone-4,6-dicarboxylate wild type protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.064
-
 2-Pyrone-4,6-dicarboxylate R183M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.066
-
 2-Pyrone-4,6-dicarboxylate D248N mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.067
-
 2-Pyrone-4,6-dicarboxylate R217M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.089
-
 2-Pyrone-4,6-dicarboxylate H33N mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.09
-
 2-Pyrone-4,6-dicarboxylate R124M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.116
-
 2-Pyrone-4,6-dicarboxylate D248A mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.214
-
 2-Pyrone-4,6-dicarboxylate H180A mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.217
-
 2-Pyrone-4,6-dicarboxylate Y49F mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.238
-
 2-Pyrone-4,6-dicarboxylate Y156F mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.465
-
 2-Pyrone-4,6-dicarboxylate R130M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.72
-
 2-Pyrone-4,6-dicarboxylate H31N mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.57 Sphingomonas paucimobilis O87170
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.57 immobilized metal ion affinity chromatography (Ni2+), gel filtration Sphingomonas paucimobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.57 2-pyrone-4,6-dicarboxylate + H2O
-
 Sphingomonas paucimobilis 4-oxalomesaconate the product occurs in two tautomeric forms. 4-oxalomesaconate is predominant at pH 10, while at pH 6 4-carboxy-2-hydroxymuconate is observed r

Synonyms

EC Number Synonyms Comment Organism
3.1.1.57 2-pyrone-4,6-dicarboxylate lactonase
-
 Sphingomonas paucimobilis
3.1.1.57 LigI
-
 Sphingomonas paucimobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.57 0.0013
-
 2-Pyrone-4,6-dicarboxylate D248A mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.02
-
 2-Pyrone-4,6-dicarboxylate R124M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.023
-
 2-Pyrone-4,6-dicarboxylate D248N mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.4
-
 2-Pyrone-4,6-dicarboxylate H31N mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.42
-
 2-Pyrone-4,6-dicarboxylate R217M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 3.6
-
 2-Pyrone-4,6-dicarboxylate H33N mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 5
-
 2-Pyrone-4,6-dicarboxylate R130M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 8
-
 2-Pyrone-4,6-dicarboxylate H180A mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 8.7
-
 2-Pyrone-4,6-dicarboxylate Y156F mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 26
-
 2-Pyrone-4,6-dicarboxylate Y49F mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 32
-
 2-Pyrone-4,6-dicarboxylate H180C mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 196
-
 2-Pyrone-4,6-dicarboxylate R183M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 342
-
 2-Pyrone-4,6-dicarboxylate wild type protein, pH 8.25, 30°C Sphingomonas paucimobilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.57 0.04
-
 5-hydroxyisophthalic acid pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.075
-
 pyridine-2,4-dicarboxylic acid pH 8.25, 30°C Sphingomonas paucimobilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.57 0.016
-
 2-Pyrone-4,6-dicarboxylate D248A mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.2
-
 2-Pyrone-4,6-dicarboxylate D248N mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.2
-
 2-Pyrone-4,6-dicarboxylate R124M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 0.5
-
 2-Pyrone-4,6-dicarboxylate H31N mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 6.3
-
 2-Pyrone-4,6-dicarboxylate R217M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 11
-
 2-Pyrone-4,6-dicarboxylate R130M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 37
-
 2-Pyrone-4,6-dicarboxylate H180A mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 37
-
 2-Pyrone-4,6-dicarboxylate Y156F mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 41
-
 2-Pyrone-4,6-dicarboxylate H33N mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 120
-
 2-Pyrone-4,6-dicarboxylate Y49F mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 780
-
 2-Pyrone-4,6-dicarboxylate H180C mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 3000
-
 2-Pyrone-4,6-dicarboxylate R183M mutant protein, pH 8.25, 30°C Sphingomonas paucimobilis
3.1.1.57 7100
-
 2-Pyrone-4,6-dicarboxylate wild type protein, pH 8.25, 30°C Sphingomonas paucimobilis