EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.27 | analysis | use of recombinant enzyme to estimate global 5-hydroxymethyl cytosine content in a variety of genomic DNAs. Most of the genomic DNAs derived from vertebrate tissue and cell lines contain 5-hydroxymethyl cytosine. DNA from mouse, human, and bovine brains displays 0.5?0.9% of the total nucleotides as 5-hydroxymethyl cytosine, which is higher compared to the levels found in other tissues. A comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer | Tequatrovirus T4 |
2.4.1.27 | medicine | a comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer | Tequatrovirus T4 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.27 | expression in Escherichia coli | Tequatrovirus T4 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.27 | 5-hydroxymethyl cytosine beta-D-glucoside containing DNA | competitive inhibitor with respect to 5-hydroxymethyl cytosine containing DNA and mixed inhibitor with respect to UDP-glucose | Tequatrovirus T4 | |
2.4.1.27 | UDP | competitive inhibitor with respect to UDP-glucose and mixed inhibitor with respect to 5-hydroxymethyl cytosine containing DNA | Tequatrovirus T4 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.27 | 0.00022 | - |
DNA containing 24 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 0.00023 | - |
DNA containing 12 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 0.0004 | - |
5-hydroxymethyl cytosine containing phage T4 DNA | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 0.0006 | - |
DNA containing 6 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 0.003 | - |
DNA containing 2 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 0.016 | - |
UDP-D-glucose | pH 7.9, 25°C | Tequatrovirus T4 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.27 | Tequatrovirus T4 | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.27 | commercial preparation | - |
Tequatrovirus T4 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.27 | additional information | enzyme shows strong preference for the glucosylation of DNA containing symmetrical 5-hydroxymethyl cytosine resiudes over hemi-5-hydroxymethyl cytosine. The enzyme is capable of saturation glucosylation on all conformations of 5-hydroxymethyl cytosine.The numbers of 5-hydroxymethyl cytosine residues on DNA can influence catalysis, especially the rate of catalysis, and hemihydroxymethylated DNA is not the preferred substrate of the enzyme | Tequatrovirus T4 | ? | - |
? | |
2.4.1.27 | UDP-D-glucose + 5-hydroxymethyl cytosine containing DNA | - |
Tequatrovirus T4 | UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing DNA | recombinant enzyme glucosylates 5-hydroxymethyl cytosine containing DNA in a nonprocessive manner, and binding to either 5-hydroxymethyl cytosine containing DNA or uridine diphosphoglucose substrates is random, with both binary complexes being catalytically competent. The numbers of 5-hydroxymethyl cytosine on target sequences influence the turnover numbers for the recombinant enzyme | ? | |
2.4.1.27 | UDP-D-glucose + 5-hydroxymethyl cytosine containing phage T4 DNA | - |
Tequatrovirus T4 | UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing phage T4 DNA | - |
? | |
2.4.1.27 | UDP-D-glucose + DNA containing 12 5-hydroxymethyl cytosine residues | - |
Tequatrovirus T4 | UDP + ? | - |
? | |
2.4.1.27 | UDP-D-glucose + DNA containing 2 5-hydroxymethyl cytosine residues | - |
Tequatrovirus T4 | UDP + ? | - |
? | |
2.4.1.27 | UDP-D-glucose + DNA containing 24 5-hydroxymethyl cytosine residues | - |
Tequatrovirus T4 | UDP + ? | - |
? | |
2.4.1.27 | UDP-D-glucose + DNA containing 6 5-hydroxymethyl cytosine residues | - |
Tequatrovirus T4 | UDP + ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.27 | 1.033 | - |
DNA containing 12 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 1.283 | - |
5-hydroxymethyl cytosine containing phage T4 DNA | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 1.666 | - |
DNA containing 6 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 2.8 | - |
DNA containing 2 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 9.66 | - |
DNA containing 24 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.27 | 0.002 | - |
UDP | substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 0.003 | - |
5-hydroxymethyl cytosine beta-D-glucoside containing DNA | substrate UDP-glucose, pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 0.004 | - |
5-hydroxymethyl cytosine beta-D-glucoside containing DNA | substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 0.009 | - |
UDP | substrate UDP-glucose, pH 7.9, 25°C | Tequatrovirus T4 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.27 | 933 | - |
DNA containing 2 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 2766 | - |
DNA containing 6 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 3200 | - |
5-hydroxymethyl cytosine containing phage T4 DNA | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 4400 | - |
DNA containing 24 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 | |
2.4.1.27 | 4483 | - |
DNA containing 12 5-hydroxymethyl cytosine residues | pH 7.9, 25°C | Tequatrovirus T4 |