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Literature summary extracted from

  • Hall, R.S.; Fedorov, A.A.; Xu, C.; Fedorov, E.V.; Almo, S.C.; Raushel, F.M.
    Three-dimensional structure and catalytic mechanism of cytosine deaminase (2011), Biochemistry, 50, 5077-5085.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.1 the structure of Zn-CDA is determined to a resolution of 1.7 A with phosphonocytosine a potent mimic of the putative tetrahedral intermediate bound in the active site Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.1 D313A metal contet: 0.57 Zn, 0.32 Fe. Mutant possesses less than 0.01% of activity of wild-type enzyme Escherichia coli
3.5.4.1 D313N metal contet: 1.05 Zn, 0.05 Fe. kcat decreased compared to wild-type, Km increased compared to wild-type Escherichia coli
3.5.4.1 E217A metal contet: 0.9 Zn, 0.08 Fe. Mutant possesses less than 0.01% of activity of wild-type enzyme Escherichia coli
3.5.4.1 E217Q metal contet: 0.87 Zn, 0.15 Fe. Mutant possesses less than 0.01% of activity of wild-type enzyme Escherichia coli
3.5.4.1 H246A metal contet: 0.59 Zn, 0.33 Fe. Mutant possesses less than 0.01% of activity of wild-type enzyme Escherichia coli
3.5.4.1 H246N metal contet: 0.53 Zn, 0.29 Fe. Mutant possesses less than 0.01% of activity of wild-type enzyme Escherichia coli
3.5.4.1 H246Q metal contet: 0.31 Zn, 0.33 Fe. kcat and Km decreased compared to wil-type Escherichia coli
3.5.4.1 Q156A metal contet: 0.92 Zn, 0.04 Fe. Mutant possesses less than 0.01% of activity of wild-type enzyme Escherichia coli
3.5.4.1 Q156N metal contet: 1.40 Zn, 0.04 Fe. Mutant possesses less than 0.01% of activity of wild-type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.1 phosphonocytosine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.1 0.25
-
 cytosine mutant H246Q, pH 8.5, 30°C Escherichia coli
3.5.4.1 0.46
-
 isocytosine wild-type, pH 8.5, 30°C Escherichia coli
3.5.4.1 0.85
-
 3-oxauracil mutant E217A, pH 8.5, 30°C Escherichia coli
3.5.4.1 0.97
-
 cytosine wild-type, pH 8.5, 30°C Escherichia coli
3.5.4.1 1.9
-
 cytosine mutant D313N, pH 8.5, 30°C Escherichia coli
3.5.4.1 4.1
-
 3-oxauracil wild-type, pH 8.5, 30°C Escherichia coli
3.5.4.1 25
-
 creatinine wild-type, pH 8.5, 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.1 Iron the enzyme contains 0.20 equivalents of Fe Escherichia coli
3.5.4.1 Zinc the enzyme contained 0.56 equivalents of Zn Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 3-oxauracil + H2O
-
 Escherichia coli malonate semialdehyde + CO2 + NH3
-
 ?
3.5.4.1 creatinine + H2O
-
 Escherichia coli N-methylhydantoin + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?
3.5.4.1 isocytosine + H2O
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.1 CDA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.1 30
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.1 0.0075
-
 cytosine mutant D313N, pH 8.5, 30°C Escherichia coli
3.5.4.1 0.18
-
 3-oxauracil mutant E217A, pH 8.5, 30°C Escherichia coli
3.5.4.1 0.18
-
 cytosine mutant H246Q, pH 8.5, 30°C Escherichia coli
3.5.4.1 2.3
-
 3-oxauracil wild-type, pH 8.5, 30°C Escherichia coli
3.5.4.1 5.1
-
 isocytosine wild-type, pH 8.5, 30°C Escherichia coli
3.5.4.1 5.6
-
 creatinine wild-type, pH 8.5, 30°C Escherichia coli
3.5.4.1 132
-
 cytosine wild-type, pH 8.5, 30°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.1 8.5
-
 assay at Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.4.1 0.000052
-
 phosphonocytosine pH 8.5, 30°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.4.1 0.004
-
 cytosine mutant D313N, pH 8.5, 30°C Escherichia coli
3.5.4.1 0.21
-
 3-oxauracil mutant E217A, pH 8.5, 30°C Escherichia coli
3.5.4.1 0.22
-
 creatinine wild-type, pH 8.5, 30°C Escherichia coli
3.5.4.1 0.56
-
 3-oxauracil wild-type, pH 8.5, 30°C Escherichia coli
3.5.4.1 0.72
-
 cytosine mutant H246Q, pH 8.5, 30°C Escherichia coli
3.5.4.1 11
-
 isocytosine wild-type, pH 8.5, 30°C Escherichia coli
3.5.4.1 140
-
 cytosine wild-type, pH 8.5, 30°C Escherichia coli