Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hartley, M.D.; Morrison, M.J.; Aas, F.E.; B?rud, B.; Koomey, M.; Imperiali, B.
    Biochemical characterization of the O-linked glycosylation pathway in Neisseria gonorrhoeae responsible for biosynthesis of protein glycans containing N,N-diacetylbacillosamine (2011), Biochemistry, 50, 4936-4948.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.203 expressed in Escherichia coli BL21 cells Neisseria gonorrhoeae
2.7.8.36 His-tagged protein expressed in Escherichia coli BL21(DE3) Neisseria gonorrhoeae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.203 membrane
-
 Neisseria gonorrhoeae 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.203 Neisseria gonorrhoeae
-
-
-
2.3.1.203 Neisseria gonorrhoeae MS11
-
-
-
2.7.8.36 Neisseria gonorrhoeae
-
-
-
2.7.8.36 Neisseria gonorrhoeae MS11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.203 Ni-NTA resin column chromatography Neisseria gonorrhoeae
2.7.8.36 preparation of cell envelope fraction, immobilized metal ion affinity chromatography (Ni2+) Neisseria gonorrhoeae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.36 UDP-N,N'-diacetylbacillosamine + undecaprenyl phosphate
-
 Neisseria gonorrhoeae UMP + N,N'-diacetylbacillosaminyl-diphospho-undecaprenol
-
 ?
2.7.8.36 UDP-N,N'-diacetylbacillosamine + undecaprenyl phosphate
-
 Neisseria gonorrhoeae MS11 UMP + N,N'-diacetylbacillosaminyl-diphospho-undecaprenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.203 PglD
-
 Neisseria gonorrhoeae
2.7.8.36 acetyltransferase/phosphoglycosyl transferase
-
 Neisseria gonorrhoeae
2.7.8.36 PglB-PGTB
-
 Neisseria gonorrhoeae
2.7.8.36 PglB-PGTD phosphoglycosyltransferase domain of PglB, PglB is a bifunctional enzyme, which catalyzes the amino acetylation of UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucose to form 2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose and the transfer of phospho-2,4-diacetamido-2,4,6-trideoxy-alpha-D-glucose to undecaprenyl phosphate Neisseria gonorrhoeae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.203 NADH dependent on Neisseria gonorrhoeae