EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.170 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces exfoliatus |
1.14.13.170 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces arenae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.170 | 66915 | - |
x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry | Streptomyces arenae |
1.14.13.170 | 67480 | - |
x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry | Streptomyces exfoliatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.36 | neopentalenolactone D + 2 2-oxoglutarate | Streptomyces exfoliatus | - |
neopentalenolactone F + methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate + ? | incubation of enzyme with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | ? | |
1.14.11.36 | neopentalenolactone D + 2 2-oxoglutarate | Streptomyces arenae | - |
neopentalenolactone F + methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate + ? | incubation of enzyme with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | ? | |
1.14.11.36 | neopentalenolactone D + 2 2-oxoglutarate | Streptomyces exfoliatus UC5319 | - |
neopentalenolactone F + methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate + ? | incubation of enzyme with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | ? | |
1.14.11.36 | neopentalenolactone D + 2 2-oxoglutarate | Streptomyces arenae TU469 | - |
neopentalenolactone F + methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate + ? | incubation of enzyme with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.36 | Streptomyces arenae | E3VWI8 | - |
- |
1.14.11.36 | Streptomyces arenae TU469 | E3VWI8 | - |
- |
1.14.11.36 | Streptomyces exfoliatus | E3VWK4 | - |
- |
1.14.11.36 | Streptomyces exfoliatus UC5319 | E3VWK4 | - |
- |
1.14.13.170 | Streptomyces arenae | - |
- |
- |
1.14.13.170 | Streptomyces arenae TU46 | - |
- |
- |
1.14.13.170 | Streptomyces exfoliatus | - |
- |
- |
1.14.13.170 | Streptomyces exfoliatus UC5319 | - |
- |
- |
1.14.13.171 | Streptomyces arenae | - |
- |
- |
1.14.13.171 | Streptomyces arenae TUE469 | - |
- |
- |
1.14.13.171 | Streptomyces avermitilis | - |
- |
- |
1.14.13.171 | Streptomyces exfoliatus | - |
- |
- |
1.14.13.171 | Streptomyces exfoliatus UC5319 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.170 | Ni-NTA column chromatography | Streptomyces exfoliatus |
1.14.13.170 | Ni-NTA column chromatography | Streptomyces arenae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.36 | neopentalenolactone D + 2 2-oxoglutarate | - |
Streptomyces exfoliatus | neopentalenolactone F + methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate + ? | incubation of enzyme with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | ? | |
1.14.11.36 | neopentalenolactone D + 2 2-oxoglutarate | - |
Streptomyces arenae | neopentalenolactone F + methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate + ? | incubation of enzyme with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | ? | |
1.14.11.36 | neopentalenolactone D + 2 2-oxoglutarate | - |
Streptomyces exfoliatus UC5319 | neopentalenolactone F + methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate + ? | incubation of enzyme with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | ? | |
1.14.11.36 | neopentalenolactone D + 2 2-oxoglutarate | - |
Streptomyces arenae TU469 | neopentalenolactone F + methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate + ? | incubation of enzyme with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | ? | |
1.14.11.36 | pentalenolactone D + 2 2-oxoglutarate | - |
Streptomyces exfoliatus | pentalenolactone F + 2 succinate + 2 CO2 + H2O | - |
? | |
1.14.11.36 | pentalenolactone D + 2 2-oxoglutarate | - |
Streptomyces arenae | pentalenolactone F + 2 succinate + 2 CO2 + H2O | - |
? | |
1.14.11.36 | pentalenolactone D + 2 2-oxoglutarate | - |
Streptomyces exfoliatus UC5319 | pentalenolactone F + 2 succinate + 2 CO2 + H2O | - |
? | |
1.14.11.36 | pentalenolactone D + 2 2-oxoglutarate | - |
Streptomyces arenae TU469 | pentalenolactone F + 2 succinate + 2 CO2 + H2O | - |
? | |
1.14.13.170 | 1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2 | - |
Streptomyces exfoliatus | pentalenolactone D + NADP+ + H2O | - |
ir | |
1.14.13.170 | 1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2 | - |
Streptomyces arenae | pentalenolactone D + NADP+ + H2O | - |
ir | |
1.14.13.170 | 1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2 | - |
Streptomyces arenae TU46 | pentalenolactone D + NADP+ + H2O | - |
ir | |
1.14.13.170 | 1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2 | - |
Streptomyces exfoliatus UC5319 | pentalenolactone D + NADP+ + H2O | - |
ir | |
1.14.13.170 | 1-deoxy-11-oxopentalenic acid + NADPH + H+ + O2 | - |
Streptomyces arenae TUE469 | pentalenolactone D + NADP+ + H2O | - |
ir | |
1.14.13.171 | 1-deoxy-11-oxopentalenate + NADPH + H+ + O2 | - |
Streptomyces exfoliatus | neopentalenolactone D + NADP+ + H2O | - |
? | |
1.14.13.171 | 1-deoxy-11-oxopentalenate + NADPH + H+ + O2 | - |
Streptomyces avermitilis | neopentalenolactone D + NADP+ + H2O | - |
? | |
1.14.13.171 | 1-deoxy-11-oxopentalenate + NADPH + H+ + O2 | - |
Streptomyces arenae | neopentalenolactone D + NADP+ + H2O | - |
? | |
1.14.13.171 | 1-deoxy-11-oxopentalenate + NADPH + H+ + O2 | - |
Streptomyces arenae TU46 | neopentalenolactone D + NADP+ + H2O | - |
? | |
1.14.13.171 | 1-deoxy-11-oxopentalenate + NADPH + H+ + O2 | - |
Streptomyces exfoliatus UC5319 | neopentalenolactone D + NADP+ + H2O | - |
? | |
1.14.13.171 | 1-deoxy-11-oxopentalenate + NADPH + H+ + O2 | - |
Streptomyces arenae TUE469 | neopentalenolactone D + NADP+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.170 | ? | x * 66915, His6-tagged enzyme, MALDI-TOF mass spectrometry | Streptomyces arenae |
1.14.13.170 | ? | x * 67480, His6-tagged enzyme, MALDI-TOF mass spectrometry | Streptomyces exfoliatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.36 | penD | - |
Streptomyces exfoliatus |
1.14.11.36 | penD | - |
Streptomyces arenae |
1.14.13.170 | Baeyer-Villiger monooxygenase | - |
Streptomyces exfoliatus |
1.14.13.170 | Baeyer-Villiger monooxygenase | - |
Streptomyces arenae |
1.14.13.170 | penE | - |
Streptomyces exfoliatus |
1.14.13.170 | pntE | - |
Streptomyces arenae |
1.14.13.171 | penE | - |
Streptomyces exfoliatus |
1.14.13.171 | pntE | - |
Streptomyces arenae |
1.14.13.171 | ptlE | - |
Streptomyces avermitilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.170 | FAD | the enzyme contains one bound FAD molecule | Streptomyces exfoliatus | |
1.14.13.170 | FAD | the enzyme contains one bound FAD molecule | Streptomyces arenae | |
1.14.13.171 | FAD | - |
Streptomyces exfoliatus | |
1.14.13.171 | FAD | - |
Streptomyces avermitilis | |
1.14.13.171 | FAD | - |
Streptomyces arenae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.11.36 | physiological function | enzyme deletion mutants accumulate pentalenolactone D but are blocked in production of pentalenolactone as well as the precursors pentalenolactones E and F. Analysis of the gene cluster responsible for pentalenolactone synthesis. Reaction starts with the flavin-dependent Baeyer-Villiger oxidation of 1-deoxy-11-oxopentalenic acid to pentalenolactone D followed by the two-stage Fe2+-alpha-ketoglutarate-dependent oxidation to pentalenolactones E and F, catalyzed by the enzyme, PntD. Incubation of PntD with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | Streptomyces arenae |
1.14.11.36 | physiological function | enzyme deletion mutants accumulate pentalenolactone D but are blocked in production of pentalenolactone as well as the precursors pentalenolactones E and F. Analysis of the gene cluster responsible for pentalenolactone synthesis. Reaction starts with the flavin-dependent Baeyer-Villiger oxidation of 1-deoxy-11-oxopentalenic acid to pentalenolactone D followed by the two-stage Fe2+-alpha-ketoglutarate-dependent oxidation to pentalenolactones E and F, catalyzed by the enzyme, PtlD. Incubation of PtlD with its natural substrate neopentalenolactone D gives a mixture of neopentalenolactone E and its derived hydrolysis product, methyl (1R,6aR)-6a-acetyl-1-(2-methoxy-2-oxoethyl)-5,5-dimethyl-1,3a,4,5,6,6a-hexahydropentalene-2-carboxylate | Streptomyces exfoliatus |