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Literature summary extracted from

  • Seiner, D.R.; Hegde, S.S.; Blanchard, J.S.
    Kinetics and inhibition of nicotinamidase from Mycobacterium tuberculosis (2010), Biochemistry, 49, 9613-9619.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.19 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.19 H57D the mutant has reduced Mn2+ content and shows a 6fold and 38fold decrease in kcat value for nicotinamide and pyrazinamide, respectively Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.19 3-pyridine carboxaldehyde competitive inhibitor Mycobacterium tuberculosis
3.5.1.19 iodoacetamide reversible, competitive inhibitor Mycobacterium tuberculosis
3.5.1.19 pyrazinecarbonitrile irreversible inactivator Mycobacterium tuberculosis
3.5.1.19 pyridine-3-carbonitrile reversible, competitive inhibitor Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.19 0.013
-
nicotinamide mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 0.014
-
nicotinamide wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 0.016
-
Pyrazinamide mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 0.3
-
Pyrazinamide wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.19 Fe2+ the enzyme contains 0.47 M of Fe2+ per mol of enzyme Mycobacterium tuberculosis
3.5.1.19 Mn2+ the enzyme contains 0.44 M Mn2+ per mol of enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Mycobacterium tuberculosis
-
nicotinate + NH3
-
?
3.5.1.19 nicotinamide + H2O Mycobacterium tuberculosis H37Rv
-
nicotinate + NH3
-
?
3.5.1.19 pyrazinamide + H2O Mycobacterium tuberculosis
-
pyrazine-2-carboxylic acid + NH3
-
?
3.5.1.19 pyrazinamide + H2O Mycobacterium tuberculosis H37Rv
-
pyrazine-2-carboxylic acid + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Mycobacterium tuberculosis
-
-
-
3.5.1.19 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.19 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O
-
Mycobacterium tuberculosis nicotinate + NH3
-
?
3.5.1.19 nicotinamide + H2O
-
Mycobacterium tuberculosis H37Rv nicotinate + NH3
-
?
3.5.1.19 pyrazinamide + H2O
-
Mycobacterium tuberculosis pyrazine-2-carboxylic acid + NH3
-
?
3.5.1.19 pyrazinamide + H2O
-
Mycobacterium tuberculosis H37Rv pyrazine-2-carboxylic acid + NH3
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.19 nicotinamidase/pyrazinamidase
-
Mycobacterium tuberculosis
3.5.1.19 PncA
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.19 0.1
-
Pyrazinamide mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 0.5
-
nicotinamide mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 3.1
-
nicotinamide wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 3.8
-
Pyrazinamide wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.19 8
-
around pH 8.0 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.19 0.00029
-
3-pyridine carboxaldehyde wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 0.1
-
pyridine-3-carbonitrile Ki about 0.1 mM, wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.5.1.19 metabolism the enzyme is involved in the NAD+ salvage pathway Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.19 6.2
-
Pyrazinamide mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 13
-
Pyrazinamide wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 37
-
nicotinamide mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis
3.5.1.19 220
-
nicotinamide wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C Mycobacterium tuberculosis