EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.19 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.19 | H57D | the mutant has reduced Mn2+ content and shows a 6fold and 38fold decrease in kcat value for nicotinamide and pyrazinamide, respectively | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.19 | 3-pyridine carboxaldehyde | competitive inhibitor | Mycobacterium tuberculosis | |
3.5.1.19 | iodoacetamide | reversible, competitive inhibitor | Mycobacterium tuberculosis | |
3.5.1.19 | pyrazinecarbonitrile | irreversible inactivator | Mycobacterium tuberculosis | |
3.5.1.19 | pyridine-3-carbonitrile | reversible, competitive inhibitor | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.19 | 0.013 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 0.014 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 0.016 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 0.3 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.19 | Fe2+ | the enzyme contains 0.47 M of Fe2+ per mol of enzyme | Mycobacterium tuberculosis | |
3.5.1.19 | Mn2+ | the enzyme contains 0.44 M Mn2+ per mol of enzyme | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.19 | nicotinamide + H2O | Mycobacterium tuberculosis | - |
nicotinate + NH3 | - |
? | |
3.5.1.19 | nicotinamide + H2O | Mycobacterium tuberculosis H37Rv | - |
nicotinate + NH3 | - |
? | |
3.5.1.19 | pyrazinamide + H2O | Mycobacterium tuberculosis | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
3.5.1.19 | pyrazinamide + H2O | Mycobacterium tuberculosis H37Rv | - |
pyrazine-2-carboxylic acid + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.19 | Mycobacterium tuberculosis | - |
- |
- |
3.5.1.19 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.19 | Ni-NTA column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.19 | nicotinamide + H2O | - |
Mycobacterium tuberculosis | nicotinate + NH3 | - |
? | |
3.5.1.19 | nicotinamide + H2O | - |
Mycobacterium tuberculosis H37Rv | nicotinate + NH3 | - |
? | |
3.5.1.19 | pyrazinamide + H2O | - |
Mycobacterium tuberculosis | pyrazine-2-carboxylic acid + NH3 | - |
? | |
3.5.1.19 | pyrazinamide + H2O | - |
Mycobacterium tuberculosis H37Rv | pyrazine-2-carboxylic acid + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.19 | nicotinamidase/pyrazinamidase | - |
Mycobacterium tuberculosis |
3.5.1.19 | PncA | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.19 | 0.1 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 0.5 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 3.1 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 3.8 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.19 | 8 | - |
around pH 8.0 | Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.19 | 0.00029 | - |
3-pyridine carboxaldehyde | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 0.1 | - |
pyridine-3-carbonitrile | Ki about 0.1 mM, wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.1.19 | metabolism | the enzyme is involved in the NAD+ salvage pathway | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.19 | 6.2 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 13 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 37 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.5.1.19 | 220 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis |