Literature summary extracted from

Thoden, J.; Holden, H.
Structural and functional studies of WlbA: A dehydrogenase involved in the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid (2010), Biochemistry, 49, 7939-7948.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.335	expression in Escherichia coli	Pseudomonas aeruginosa
1.1.1.335	expression in Escherichia coli	Thermus thermophilus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.1.1.335	crystal structure of the enzyme in a complex with NAD(H), to 1.5 A resolution. The tetrameric enzyme assumes an unusual quaternary structure with the dinucleotides positioned quite closely to one another. Both 2-oxoglutarate and the UDP-linked sugar bind in the enzyme active site with their carbon atoms, C-2 and C-3', respectively, abutting the re face of the cofactor. They are positioned about 3 A from the nicotinamide C-4. The UDP-linked sugar substrate adopts a highly unusual curved conformation when bound in the enzyme's active site cleft	Pseudomonas aeruginosa
1.1.1.335	crystal structures of the enzyme in a complex with NAD(H) and 2-oxoglutarate, and the enzyme in a complex with NAD(H) and its substrate UDP-N-acetyl-D-glucosaminuronic acid, to 1.45 A and 2.0 A resolution, respectively. The tetrameric enzyme assumes an unusual quaternary structure with the dinucleotides positioned quite closely to one another. Both 2-oxoglutarate and the UDP-linked sugar bind in the enzyme active site with their carbon atoms, C-2 and C-3', respectively, abutting the re face of the cofactor. They are positioned about 3 A from the nicotinamide C-4. The UDP-linked sugar substrate adopts a highly unusual curved conformation when bound in the enzyme's active site cleft. Residues Lys101 and His185 most likely play key roles in catalysis	Thermus thermophilus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.335	Pseudomonas aeruginosa	G3XD23	-	-
1.1.1.335	Thermus thermophilus	Q72KX8	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.335	WblA	-	Pseudomonas aeruginosa
1.1.1.335	WblA	-	Thermus thermophilus

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Release 2023.1 (January 2023)