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Literature summary extracted from

  • Yang, J.; Rawat, S.; Stemmler, T.L.; Rosen, B.P.
    Arsenic binding and transfer by the ArsD As(III) metallochaperone (2010), Biochemistry, 49, 3658-3666.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.3.2.7 ArsD a metallochaperone that delivers trivalent metalloids [As(III) or Sb(III)] to the ArsA ATPase, the catalytic subunit of the ArsAB pump. ArsD residues Cys12, Cys13, and Cys18 are involved in the transfer of As(III) to ArsA Escherichia coli
7.3.2.7 additional information in vivo, cytosolic As(III) is nearly completely complexed with GSH. GSH greatly increases the rate of binding of As(III) to ArsD, but GSH does not affect the As(III)-stimulated ArsA ATPase activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.7 ATP + H2O + antimonite/in Escherichia coli
-
 ADP + phosphate + antimonite/out
-
 ?
7.3.2.7 ATP + H2O + arsenite/in Escherichia coli
-
 ADP + phosphate + arsenite/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.7 Escherichia coli
-
 ArsAB pump is encoded in arsRDABC operon on plasmid R773
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.7 ATP + H2O + antimonite/in
-
 Escherichia coli ADP + phosphate + antimonite/out
-
 ?
7.3.2.7 ATP + H2O + arsenite/in
-
 Escherichia coli ADP + phosphate + arsenite/out
-
 ?
7.3.2.7 ATP + H2O + arsenite/in ArsA has a high-affinity metalloid binding site composed of Cys113 and Cys422, and a third residue, Cys172, that participates in high-affinity binding and activation of ATP hydrolysis Escherichia coli ADP + phosphate + arsenite/out
-
 ?
7.3.2.7 additional information ArsA exhibits a low, basal rate of ATPase activity in the absence of As(III) or Sb(III) and a higher, activated rate in their presence Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.7 ArsAB pump
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.3.2.7 37
-
 ATPase assay at Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
7.3.2.7 additional information
-
 transfer of As(III) from ArsD to ArsA occurs in the presence of MgATP at 23°C but not at 4°C Escherichia coli

General Information

EC Number General Information Comment Organism
7.3.2.7 additional information ArsD is a metallochaperone that delivers trivalent metalloids [As(III) or Sb(III)] to the ArsA ATPase, the catalytic subunit of the ArsAB pump. ArsD residues Cys12, Cys13, and Cys18 are involved in the transfer of As(III) to ArsA. Transfer of As(III) from ArsD to ArsA occurs in the presence of MgATP, neither MgADP nor MgATP-gamma-S can replace MgATP. Transfer occurs with a conformation of ArsA that transiently forms during the catalytic cycle and not simply to the closed conformation that ArsA adopts when As(III) and MgATP are bound Escherichia coli