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Literature summary extracted from

  • Huang, X.; Gibson, L.M.; Bell, B.J.; Lovelace, L.L.; Pena, M.M.; Berger, F.G.; Berger, S.H.; Lebioda, L.
    Replacement of Val3 in human thymidylate synthase affects its kinetic properties and intracellular stability (2010), Biochemistry, 49, 2475-2482.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 expressed in Escherichia coli strain TX61- cells and in the RJK88.13 cell line (an enzyme-deficient derivative of V79 Chinese hamster lung cells) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45 V3L, V3F and V3Y mutant enzymes, hanging drop vapor diffusion method, using 100 mM Tris-base, pH 8.5, 20 mM 2-mercaptoethanol, 32-40% (w/v) ammonium sulfate and 16-24% (w/v) polyethylene glycol 4000 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.45 V3A the mutant enzyme has an intracellular half-life of approximately 6 h after treatment with cycloheximide Homo sapiens
2.1.1.45 V3F the mutant enzyme has an intracellular half-life of approximately 3.5 h after treatment with cycloheximide Homo sapiens
2.1.1.45 V3L the mutant enzyme has an intracellular half-life of approximately 2.5 h after treatment with cycloheximide Homo sapiens
2.1.1.45 V3T the mutant enzyme has an intracellular half-life of approximately 24 h after treatment with cycloheximide Homo sapiens
2.1.1.45 V3Y the mutant enzyme has an intracellular half-life of approximately 2.5 h after treatment with cycloheximide Homo sapiens

General Stability

EC Number General Stability Organism
2.1.1.45 the wild type enzyme has an intracellular half-life of approximately 6 h after treatment with cycloheximide Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0023
-
 dUMP apparent value, wild type enzyme, pH not specified in the publication, at 37°C Homo sapiens
2.1.1.45 0.0044
-
 dUMP apparent value, mutant enzyme V3Y, pH not specified in the publication, at 37°C Homo sapiens
2.1.1.45 0.011
-
 dUMP apparent value, mutant enzyme V3T, pH not specified in the publication, at 37°C Homo sapiens
2.1.1.45 0.022
-
 dUMP apparent value, mutant enzyme V3A, pH not specified in the publication, at 37°C Homo sapiens
2.1.1.45 0.109
-
 dUMP apparent value, mutant enzyme V3L, pH not specified in the publication, at 37°C Homo sapiens
2.1.1.45 0.134
-
 dUMP apparent value, mutant enzyme V3F, pH not specified in the publication, at 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 homodimer x-ray crystallography Homo sapiens