BRENDA - Enzyme Database show

Characterization of nicotinamidases: steady state kinetic parameters, classwide inhibition by nicotinaldehydes, and catalytic mechanism

French, J.B.; Cen, Y.; Vrablik, T.L.; Xu, P.; Allen, E.; Hanna-Rose, W.; Sauve, A.A.; Biochemistry 49, 10421-10439 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.19
-
Borreliella burgdorferi
3.5.1.19
-
Caenorhabditis elegans
3.5.1.19
-
Plasmodium falciparum
3.5.1.19
-
Saccharomyces cerevisiae
3.5.1.19
-
Streptococcus pneumoniae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
C136A
the mutant is unable to catalyze nicotinamide hydrolysis
Streptococcus pneumoniae
3.5.1.19
C136S
the mutant is unable to catalyze nicotinamide hydrolysis
Streptococcus pneumoniae
3.5.1.19
K103A
the mutant has only 0.15% of the catalytic activity of the native enzyme
Streptococcus pneumoniae
3.5.1.19
R97A
the mutant is a robust nicotinamidase and is nearly as good as the native enzyme
Streptococcus pneumoniae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
2-chloropyridine-3-carbaldehyde
-
Borreliella burgdorferi
3.5.1.19
2-chloropyridine-3-carbaldehyde
-
Saccharomyces cerevisiae
3.5.1.19
3-acetylpyridine
-
Borreliella burgdorferi
3.5.1.19
3-acetylpyridine
-
Plasmodium falciparum
3.5.1.19
3-acetylpyridine
-
Saccharomyces cerevisiae
3.5.1.19
3-acetylpyridine
-
Streptococcus pneumoniae
3.5.1.19
3-Cyanopyridine
-
Borreliella burgdorferi
3.5.1.19
3-Cyanopyridine
-
Plasmodium falciparum
3.5.1.19
3-Cyanopyridine
-
Saccharomyces cerevisiae
3.5.1.19
3-Cyanopyridine
-
Streptococcus pneumoniae
3.5.1.19
3-hydroxypyridine
-
Streptococcus pneumoniae
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Borreliella burgdorferi
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Caenorhabditis elegans
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Plasmodium falciparum
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Saccharomyces cerevisiae
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Streptococcus pneumoniae
3.5.1.19
5-bromopyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
3.5.1.19
5-bromopyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Borreliella burgdorferi
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Caenorhabditis elegans
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Streptococcus pneumoniae
3.5.1.19
5-methylnicotinaldehyde
-
Borreliella burgdorferi
3.5.1.19
5-methylnicotinaldehyde
-
Caenorhabditis elegans
3.5.1.19
5-methylnicotinaldehyde
-
Plasmodium falciparum
3.5.1.19
5-methylnicotinaldehyde
-
Saccharomyces cerevisiae
3.5.1.19
5-methylnicotinaldehyde
-
Streptococcus pneumoniae
3.5.1.19
5-methylpyridine-3-carbaldehyde
potent inhibitor
Borreliella burgdorferi
3.5.1.19
5-methylpyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
3.5.1.19
5-methylpyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
3.5.1.19
6-fluoropyridine-3-carbaldehyde
-
Borreliella burgdorferi
3.5.1.19
6-fluoropyridine-3-carbaldehyde
-
Plasmodium falciparum
3.5.1.19
additional information
not inhibited by 3-hydroxypyridine
Borreliella burgdorferi
3.5.1.19
additional information
not inhibited by nicotinic acid
Caenorhabditis elegans
3.5.1.19
additional information
not inhibited by 3-hydroxypyridine and 2-chloropyridine-3-carbaldehyde
Plasmodium falciparum
3.5.1.19
additional information
not inhibited by 3-hydroxypyridine and 6-fluoropyridine-3-carbaldehyde
Saccharomyces cerevisiae
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Borreliella burgdorferi
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Caenorhabditis elegans
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Plasmodium falciparum
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Saccharomyces cerevisiae
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Streptococcus pneumoniae
3.5.1.19
nicotinic acid
-
Borreliella burgdorferi
3.5.1.19
nicotinic acid
-
Plasmodium falciparum
3.5.1.19
nicotinic acid
-
Saccharomyces cerevisiae
3.5.1.19
nicotinic acid
-
Streptococcus pneumoniae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0027
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
0.003
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.0106
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0367
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.076
-
nicotinamide
wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.113
-
nicotinamide
wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
Plasmodium falciparum
12% activity compared to nicotinamide
?
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
Borreliella burgdorferi
20% activity compared to nicotinamide
?
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
5% activity compared to nicotinamide
?
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
Streptococcus pneumoniae
6.5% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
Streptococcus pneumoniae
137% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
206% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
Borreliella burgdorferi
221% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
Plasmodium falciparum
646% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
Streptococcus pneumoniae
163% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
Plasmodium falciparum
330% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
Borreliella burgdorferi
51% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
80% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
20% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
Streptococcus pneumoniae
36% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
Plasmodium falciparum
45% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-chloropyridine-3-carboxamide + H2O
Streptococcus pneumoniae
1.8% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-chloropyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
17% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
Streptococcus pneumoniae
11% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
Plasmodium falciparum
40% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
Borreliella burgdorferi
45% activity compared to nicotinamide
?
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
0.86% activity compared to nicotinamide
?
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
2.3% activity compared to nicotinamide
?
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
4.4% activity compared to nicotinamide
?
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
15% activity compared to nicotinamide
?
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
3.5% activity compared to nicotinamide
?
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
6500% activity compared to nicotinamide
?
-
-
?
3.5.1.19
additional information
Saccharomyces cerevisiae
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
3.5.1.19
additional information
Plasmodium falciparum
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
3.5.1.19
additional information
Borreliella burgdorferi
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
3.5.1.19
additional information
Streptococcus pneumoniae
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
?
-
-
-
3.5.1.19
additional information
Caenorhabditis elegans
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
?
-
-
-
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Streptococcus pneumoniae
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Caenorhabditis elegans
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Plasmodium falciparum
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Borreliella burgdorferi
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
13% activity compared to nicotinamide
?
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
3.1% activity compared to nicotinamide
?
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
3.1% activity compared to nicotinamide
?
-
-
?
3.5.1.19
pyrazinamide + H2O
Saccharomyces cerevisiae
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Streptococcus pneumoniae
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Caenorhabditis elegans
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Plasmodium falciparum
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Borreliella burgdorferi
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyridine-3-carbothioamide + H2O
Saccharomyces cerevisiae
44% activity compared to nicotinamide
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Borreliella burgdorferi
-
-
-
3.5.1.19
Caenorhabditis elegans
-
-
-
3.5.1.19
Plasmodium falciparum
-
-
-
3.5.1.19
Saccharomyces cerevisiae
-
-
-
3.5.1.19
Streptococcus pneumoniae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
-
Borreliella burgdorferi
3.5.1.19
-
Caenorhabditis elegans
3.5.1.19
-
Plasmodium falciparum
3.5.1.19
-
Saccharomyces cerevisiae
3.5.1.19
-
Streptococcus pneumoniae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
12% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
20% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
5% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
6.5% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
137% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
206% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
221% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
646% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
163% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
330% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
51% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
80% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
20% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
36% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
45% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
6-chloropyridine-3-carboxamide + H2O
1.8% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
6-chloropyridine-3-carboxamide + H2O
17% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
11% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
40% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
45% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
0.86% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
2.3% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
4.4% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
15% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
3.5% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
6500% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
-
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Plasmodium falciparum
?
-
-
-
-
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Borreliella burgdorferi
?
-
-
-
-
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
-
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
718859
Caenorhabditis elegans
?
-
-
-
-
3.5.1.19
nicotinamide + H2O
100% activity
718859
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
100% activity
718859
Streptococcus pneumoniae
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
100% activity
718859
Caenorhabditis elegans
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
100% activity
718859
Plasmodium falciparum
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
100% activity
718859
Borreliella burgdorferi
nicotinate + NH3
-
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
13% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
3.1% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
3.1% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Saccharomyces cerevisiae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Streptococcus pneumoniae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Caenorhabditis elegans
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Plasmodium falciparum
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Borreliella burgdorferi
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyridine-3-carbothioamide + H2O
44% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.003
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.28
-
nicotinamide
wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.3
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
2.1
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
3.1
-
nicotinamide
wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
3.8
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.19
0.000011
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
0.000022
-
nicotinaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.000023
-
5-methylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.000023
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.000034
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.000039
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.000056
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
0.00011
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.00011
-
nicotinaldehyde
isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.00014
-
5-methoxypyridine-3-carbaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.00019
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.00031
-
5-methoxypyridine-3-carbaldehyde
isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.00044
-
4-methoxylnicotinaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.00059
-
5-bromopyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.00065
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.00085
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.001
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.0014
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0038
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.004
-
5-bromopyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.004
-
6-fluoropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.018
-
6-fluoropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.05
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
0.068
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.085
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.153
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.37
-
2-chloropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.5
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
1
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
2
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
2
-
nicotinic acid
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
5
-
2-chloropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
-
Borreliella burgdorferi
3.5.1.19
-
Caenorhabditis elegans
3.5.1.19
-
Plasmodium falciparum
3.5.1.19
-
Saccharomyces cerevisiae
3.5.1.19
-
Streptococcus pneumoniae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
C136A
the mutant is unable to catalyze nicotinamide hydrolysis
Streptococcus pneumoniae
3.5.1.19
C136S
the mutant is unable to catalyze nicotinamide hydrolysis
Streptococcus pneumoniae
3.5.1.19
K103A
the mutant has only 0.15% of the catalytic activity of the native enzyme
Streptococcus pneumoniae
3.5.1.19
R97A
the mutant is a robust nicotinamidase and is nearly as good as the native enzyme
Streptococcus pneumoniae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.19
2-chloropyridine-3-carbaldehyde
-
Borreliella burgdorferi
3.5.1.19
2-chloropyridine-3-carbaldehyde
-
Saccharomyces cerevisiae
3.5.1.19
3-acetylpyridine
-
Borreliella burgdorferi
3.5.1.19
3-acetylpyridine
-
Plasmodium falciparum
3.5.1.19
3-acetylpyridine
-
Saccharomyces cerevisiae
3.5.1.19
3-acetylpyridine
-
Streptococcus pneumoniae
3.5.1.19
3-Cyanopyridine
-
Borreliella burgdorferi
3.5.1.19
3-Cyanopyridine
-
Plasmodium falciparum
3.5.1.19
3-Cyanopyridine
-
Saccharomyces cerevisiae
3.5.1.19
3-Cyanopyridine
-
Streptococcus pneumoniae
3.5.1.19
3-hydroxypyridine
-
Streptococcus pneumoniae
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Borreliella burgdorferi
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Caenorhabditis elegans
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Plasmodium falciparum
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Saccharomyces cerevisiae
3.5.1.19
4-methoxylnicotinaldehyde
weak inhibitor
Streptococcus pneumoniae
3.5.1.19
5-bromopyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
3.5.1.19
5-bromopyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Borreliella burgdorferi
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Caenorhabditis elegans
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
3.5.1.19
5-methoxypyridine-3-carbaldehyde
potent inhibitor
Streptococcus pneumoniae
3.5.1.19
5-methylnicotinaldehyde
-
Borreliella burgdorferi
3.5.1.19
5-methylnicotinaldehyde
-
Caenorhabditis elegans
3.5.1.19
5-methylnicotinaldehyde
-
Plasmodium falciparum
3.5.1.19
5-methylnicotinaldehyde
-
Saccharomyces cerevisiae
3.5.1.19
5-methylnicotinaldehyde
-
Streptococcus pneumoniae
3.5.1.19
5-methylpyridine-3-carbaldehyde
potent inhibitor
Borreliella burgdorferi
3.5.1.19
5-methylpyridine-3-carbaldehyde
potent inhibitor
Plasmodium falciparum
3.5.1.19
5-methylpyridine-3-carbaldehyde
potent inhibitor
Saccharomyces cerevisiae
3.5.1.19
6-fluoropyridine-3-carbaldehyde
-
Borreliella burgdorferi
3.5.1.19
6-fluoropyridine-3-carbaldehyde
-
Plasmodium falciparum
3.5.1.19
additional information
not inhibited by 3-hydroxypyridine
Borreliella burgdorferi
3.5.1.19
additional information
not inhibited by nicotinic acid
Caenorhabditis elegans
3.5.1.19
additional information
not inhibited by 3-hydroxypyridine and 2-chloropyridine-3-carbaldehyde
Plasmodium falciparum
3.5.1.19
additional information
not inhibited by 3-hydroxypyridine and 6-fluoropyridine-3-carbaldehyde
Saccharomyces cerevisiae
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Borreliella burgdorferi
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Caenorhabditis elegans
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Plasmodium falciparum
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Saccharomyces cerevisiae
3.5.1.19
nicotinaldehyde
potent competitive inhibitor
Streptococcus pneumoniae
3.5.1.19
nicotinic acid
-
Borreliella burgdorferi
3.5.1.19
nicotinic acid
-
Plasmodium falciparum
3.5.1.19
nicotinic acid
-
Saccharomyces cerevisiae
3.5.1.19
nicotinic acid
-
Streptococcus pneumoniae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.1.19
0.000011
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
0.000022
-
nicotinaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.000023
-
5-methylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.000023
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.000034
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.000039
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.000056
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
0.00011
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.00011
-
nicotinaldehyde
isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.00014
-
5-methoxypyridine-3-carbaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.00019
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.00031
-
5-methoxypyridine-3-carbaldehyde
isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.00044
-
4-methoxylnicotinaldehyde
isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.00059
-
5-bromopyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.00065
-
5-methylpyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.00085
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.001
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.0014
-
nicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0038
-
5-methoxypyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.004
-
5-bromopyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.004
-
6-fluoropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.018
-
6-fluoropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.05
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
0.068
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.085
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.153
-
4-methoxylnicotinaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.37
-
2-chloropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.5
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
1
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
2
-
3-Cyanopyridine
in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
2
-
nicotinic acid
in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
5
-
2-chloropyridine-3-carbaldehyde
in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.0027
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
0.003
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
0.0106
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
3.5.1.19
0.0367
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.076
-
nicotinamide
wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.113
-
nicotinamide
wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
Plasmodium falciparum
12% activity compared to nicotinamide
?
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
Borreliella burgdorferi
20% activity compared to nicotinamide
?
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
5% activity compared to nicotinamide
?
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
Streptococcus pneumoniae
6.5% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
Streptococcus pneumoniae
137% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
206% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
Borreliella burgdorferi
221% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
Plasmodium falciparum
646% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
Streptococcus pneumoniae
163% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
Plasmodium falciparum
330% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
Borreliella burgdorferi
51% activity compared to nicotinamide
?
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
80% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
20% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
Streptococcus pneumoniae
36% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
Plasmodium falciparum
45% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-chloropyridine-3-carboxamide + H2O
Streptococcus pneumoniae
1.8% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-chloropyridine-3-carboxamide + H2O
Saccharomyces cerevisiae
17% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
Streptococcus pneumoniae
11% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
Plasmodium falciparum
40% activity compared to nicotinamide
?
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
Borreliella burgdorferi
45% activity compared to nicotinamide
?
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
0.86% activity compared to nicotinamide
?
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
2.3% activity compared to nicotinamide
?
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
4.4% activity compared to nicotinamide
?
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
15% activity compared to nicotinamide
?
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
3.5% activity compared to nicotinamide
?
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
6500% activity compared to nicotinamide
?
-
-
?
3.5.1.19
additional information
Saccharomyces cerevisiae
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
3.5.1.19
additional information
Plasmodium falciparum
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
3.5.1.19
additional information
Borreliella burgdorferi
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
?
-
-
-
3.5.1.19
additional information
Streptococcus pneumoniae
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
?
-
-
-
3.5.1.19
additional information
Caenorhabditis elegans
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
?
-
-
-
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Streptococcus pneumoniae
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Caenorhabditis elegans
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Plasmodium falciparum
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
nicotinamide + H2O
Borreliella burgdorferi
100% activity
nicotinate + NH3
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
Plasmodium falciparum
13% activity compared to nicotinamide
?
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
Saccharomyces cerevisiae
3.1% activity compared to nicotinamide
?
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
Streptococcus pneumoniae
3.1% activity compared to nicotinamide
?
-
-
?
3.5.1.19
pyrazinamide + H2O
Saccharomyces cerevisiae
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Streptococcus pneumoniae
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Caenorhabditis elegans
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Plasmodium falciparum
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyrazinamide + H2O
Borreliella burgdorferi
-
pyrazine-2-carboxylic acid + NH3
-
-
?
3.5.1.19
pyridine-3-carbothioamide + H2O
Saccharomyces cerevisiae
44% activity compared to nicotinamide
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
-
Borreliella burgdorferi
3.5.1.19
-
Caenorhabditis elegans
3.5.1.19
-
Plasmodium falciparum
3.5.1.19
-
Saccharomyces cerevisiae
3.5.1.19
-
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
12% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
20% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
5% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
4-methoxypyridine-3-carboxamide + H2O
6.5% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
137% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
206% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
221% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
3.5.1.19
5-methoxypyridine-3-carboxamide + H2O
646% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
163% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
330% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
51% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
3.5.1.19
5-methylpyridine-3-carboxamide + H2O
80% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
20% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
36% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
6-aminopyridine-3-carboxamide + H2O
45% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
6-chloropyridine-3-carboxamide + H2O
1.8% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
6-chloropyridine-3-carboxamide + H2O
17% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
11% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
40% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
6-methylpyridine-3-carboxamide + H2O
45% activity compared to nicotinamide
718859
Borreliella burgdorferi
?
-
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
0.86% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
2.3% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
ethyl pyridine-3-carboxylate + H2O
4.4% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
15% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
3.5% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
methyl pyridine-3-carboxylate + H2O
6500% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
-
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Plasmodium falciparum
?
-
-
-
-
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methyl isonicotinamide
718859
Borreliella burgdorferi
?
-
-
-
-
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
-
3.5.1.19
additional information
nicotinamidase cannot hydrolyze 1-methylisonicotinamide
718859
Caenorhabditis elegans
?
-
-
-
-
3.5.1.19
nicotinamide + H2O
100% activity
718859
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
100% activity
718859
Streptococcus pneumoniae
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
100% activity
718859
Caenorhabditis elegans
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
100% activity
718859
Plasmodium falciparum
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
100% activity
718859
Borreliella burgdorferi
nicotinate + NH3
-
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
13% activity compared to nicotinamide
718859
Plasmodium falciparum
?
-
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
3.1% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
3.5.1.19
phenyl pyridine-3-carboxylate + H2O
3.1% activity compared to nicotinamide
718859
Streptococcus pneumoniae
?
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Saccharomyces cerevisiae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Streptococcus pneumoniae
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Caenorhabditis elegans
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Plasmodium falciparum
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
718859
Borreliella burgdorferi
pyrazine-2-carboxylic acid + NH3
-
-
-
?
3.5.1.19
pyridine-3-carbothioamide + H2O
44% activity compared to nicotinamide
718859
Saccharomyces cerevisiae
?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.003
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Borreliella burgdorferi
3.5.1.19
0.28
-
nicotinamide
wild type enzyme isoform PNC2, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
0.3
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Plasmodium falciparum
3.5.1.19
2.1
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Streptococcus pneumoniae
3.5.1.19
3.1
-
nicotinamide
wild type enzyme isoform PNC1, in 100 mM phosphate buffer, pH 7.3 at 25°C
Caenorhabditis elegans
3.5.1.19
3.8
-
nicotinamide
wild type enzyme, in 100 mM phosphate buffer, pH 7.3 at 25°C
Saccharomyces cerevisiae
General Information
EC Number
General Information
Commentary
Organism
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Borreliella burgdorferi
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Caenorhabditis elegans
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Plasmodium falciparum
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Saccharomyces cerevisiae
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Streptococcus pneumoniae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Borreliella burgdorferi
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Caenorhabditis elegans
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Plasmodium falciparum
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Saccharomyces cerevisiae
3.5.1.19
physiological function
consistent with its centrality to NAD+ homeostasis, nicotinamidase is essential for viability
Streptococcus pneumoniae