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Literature summary extracted from

  • Chen, M.M.; Weerapana, E.; Ciepichal, E.; Stupak, J.; Reid, C.W.; Swiezewska, E.; Imperiali, B.
    Polyisoprenol specificity in the Campylobacter jejuni N-linked glycosylation pathway (2007), Biochemistry, 46, 14342-14348.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.291 gene pglJ, expression of His-tagged PglJ in Escherichia coli Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.291 additional information
-
 additional information kinetics and substrate specificity, overview Campylobacter jejuni

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.291 plasma membrane associated, the enzyme functions at a membrane interface on the cytoplasmic side Campylobacter jejuni 5886
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.291 Campylobacter jejuni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.291 recombinant His-tagged PglJ from Escherichia coli by nickel affinity chromatography and gel filtration Campylobacter jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.291 additional information with polyisoprenols substrates of varying length, the enzyme exhibits specificity with respect to cis-double bond geometry and alpha-unsaturation of the native substrate are important features, while the precise polyisoprene length may be less critical. PglJ accepts the prenol-9 substrate with efficiencies equal to that of the native undecaprenol-based substrate Campylobacter jejuni ?
-
 ?
2.4.1.291 UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol in the assay, radiolabeled UDP-GalNAc is used with radiolabeled polyisoprenyl diphosphate Bac2,4diNAc-[3H]GalNAc, enzymatically synthesized Campylobacter jejuni UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.291 PglJ
-
 Campylobacter jejuni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.291 22
-
 assay at Campylobacter jejuni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.291 8
-
 assay at Campylobacter jejuni

General Information

EC Number General Information Comment Organism
2.4.1.291 physiological function the enzyme is involved in a general N-linked glycosylation pathway in which a heptasaccharide is sequentially assembled onto a polyisoprenyl diphosphate carrier, anchored at the cytoplasmic face of the inner membrane, and subsequently transferred to the asparagine side chain of an acceptor protein. The polyisoprenyl carrier plays a specific role in the function of these enzymes beyond a purely physical role as a membrane anchor Campylobacter jejuni