Any feedback?
Literature summary extracted from
- Crystal structure of type 1 ribonuclease H from hyperthermophilic archaeon Sulfolobus tokodaii: role of arginine 118 and C-terminal anchoring (2007), Biochemistry, 46, 11494-11503.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.26.4 | crystal structure is determined at 1.6 A resolution, crystals are grown by the sitting-drop vapor-diffusion method at 4°C | Sulfurisphaera tokodaii |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.26.4 | R118A | the specific activities of R118A-RNase HI for both 12-bp RNA/DNA and RNA/RNA substrates are reduced by approximately 10fold as compared to those of the wild-type protein | Sulfurisphaera tokodaii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.26.4 | Sulfurisphaera tokodaii | F9VN79 | - |
- |
| 3.1.26.4 | Sulfurisphaera tokodaii 7 | F9VN79 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.26.4 | RNA/DNA hybrid + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? |  |
| 3.1.26.4 | RNA/DNA hybrid + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.26.4 | ribonuclease HI | - |
Sulfurisphaera tokodaii |
| 3.1.26.4 | Sto-RNase HI | - |
Sulfurisphaera tokodaii |
| 3.1.26.4 | type 1 ribonuclease H | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | 37 | - |
assay at | Sulfurisphaera tokodaii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | 78 | - |
Tm-value for mutant enzyme DELTAC6 | Sulfurisphaera tokodaii |
| 3.1.26.4 | 93 | - |
Tm-value for mutant enzyme C58/145A | Sulfurisphaera tokodaii |
| 3.1.26.4 | 102 | - |
Tm-value for wild-type enzyme. The thermostable protein is destabilized by elimination of the disulfide bond and C-terminal truncation | Sulfurisphaera tokodaii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.26.4 | 8.5 | - |
assay at | Sulfurisphaera tokodaii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
