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Literature summary extracted from

  • Hallis, T.M.; Lei, Y.; Que, N.L.; Liu, H.
    Mechanistic studies of the biosynthesis of paratose: purification and characterization of CDP-paratose synthase (1998), Biochemistry, 37, 4935-4945.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.342 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhi

General Stability

EC Number General Stability Organism
1.1.1.342 enzyme is very unstable at low protein concentration Salmonella enterica subsp. enterica serovar Typhi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.342 adenosine triphosphate ribose competitive inhibitor with respect to NADPH and either an uncompetitive or a mixed type inhibitor with respect to CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose Salmonella enterica subsp. enterica serovar Typhi
1.1.1.342 CDP competitive inhibitor with respect to CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose and either an uncompetitive or a mixed type inhibitor with respect to NADPH Salmonella enterica subsp. enterica serovar Typhi
1.1.1.342 CDP-paratose competitive inhibitor against CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose and mixed type or uncompetitive inhibitor with respect to NADPH Salmonella enterica subsp. enterica serovar Typhi
1.1.1.342 NADP+ mixed type or uncompetitive inhibitor with respect to CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose and competitive inhibitor for NADPH Salmonella enterica subsp. enterica serovar Typhi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.342 0.026
-
 NADPH pH 8.0, 25°C Salmonella enterica subsp. enterica serovar Typhi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.342 29000
-
 2 * 29000, SDS-PAGE, 2 * 31501, calculated Salmonella enterica subsp. enterica serovar Typhi
1.1.1.342 31501
-
 2 * 29000, SDS-PAGE, 2 * 31501, calculated Salmonella enterica subsp. enterica serovar Typhi
1.1.1.342 55000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhi

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.342 Salmonella enterica subsp. enterica serovar Typhi
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.342 93
-
 pH 8.0, 25°C Salmonella enterica subsp. enterica serovar Typhi

Storage Stability

EC Number Storage Stability Organism
1.1.1.342 -80°C, stable for at least 1 year Salmonella enterica subsp. enterica serovar Typhi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.342 CDP-4-dehydro-3,6-dideoxy-alpha-D-glucose + NADPH + H+
-
 Salmonella enterica subsp. enterica serovar Typhi CDP-alpha-D-paratose + NADP+ conversion is almost quantitative ?
1.1.1.342 additional information enzyme catalyzes the stereospecific hydride transfer of the pro-S hydrogen from the C-4' position of the reduced coenzyme to C-4 of the substrate, CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose. The overall equilibrium of this catalysis greatly favors the formation of the reduced sugar product and the oxidized coenzyme Salmonella enterica subsp. enterica serovar Typhi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.342 dimer 2 * 29000, SDS-PAGE, 2 * 31501, calculated Salmonella enterica subsp. enterica serovar Typhi

Synonyms

EC Number Synonyms Comment Organism
1.1.1.342 rfbS
-
 Salmonella enterica subsp. enterica serovar Typhi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.342 7
-
 buffer 0.2 M potassium phosphate Salmonella enterica subsp. enterica serovar Typhi

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.342 NADH NADPH is 10fold prefered over NADH Salmonella enterica subsp. enterica serovar Typhi
1.1.1.342 NADPH NADPH is 10fold prefered over NADH. Enzyme exhibits a high affinity for NADPH with a much smaller dissociation constant of 0.005microM compared to the Km of 0.26 mM Salmonella enterica subsp. enterica serovar Typhi