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Literature summary extracted from

  • Zhao, S.; Roberts, D.; Ragsdale, S.
    Mechanistic studies of the methyltransferase from Clostridium thermoaceticum: Origin of the pH dependence of the methyl group transfer from methyltetrahydrofolate to the corrinoid/iron-sulfur protein (1995), Biochemistry, 34, 15075-15083.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.258 0.01
-
 5-methyltetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 0.01
-
 5-methyltetrahydrofolate using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 0.06
-
 [Co(I) corrinoid Fe-S protein] in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 0.1
-
 [methyl-Co(III) corrinoid Fe-S protein] Km above 0.1 mM, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 0.2
-
 tetrahydrofolate Km above 0.2 mM, using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 0.341
-
 tetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 0.665
-
 methylcobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 2
-
 hydroxocobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.258 Moorella thermoacetica
-
 formerly Clostridium thermoaceticum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.258
-
 Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.258 hydroxocobalamin + 5-methyltetrahydrofolate
-
 Moorella thermoacetica methylcobalamin + tetrahydrofolate
-
 r
2.1.1.258 methylcobalamin + tetrahydrofolate
-
 Moorella thermoacetica hydroxocobalamin + 5-methyltetrahydrofolate
-
 r
2.1.1.258 [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
-
 Moorella thermoacetica [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate
-
 r
2.1.1.258 [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate
-
 Moorella thermoacetica [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.1.1.258 methyltetrahydrofo1ate:corrinoid/iron-sulfur protein methyltransferase
-
 Moorella thermoacetica
2.1.1.258 MeTr
-
 Moorella thermoacetica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.258 0.02
-
 [methyl-Co(III) corrinoid Fe-S protein] kcat below 0.02 s(-1), in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 1.1
-
 hydroxocobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 1.5
-
 5-methyltetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 2.2
-
 methylcobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 18
-
 [Co(I) corrinoid Fe-S protein] in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.258 Moorella thermoacetica isoelectric focusing
-
 4.8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.258 2.1
-
 methylcobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 4.4
-
 tetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 5.2
-
 tetrahydrofolate using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 14.7
-
 hydroxocobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 17.1
-
 [methyl-Co(III) corrinoid Fe-S protein] in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 450
-
 5-methyltetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 1600
-
 [Co(I) corrinoid Fe-S protein] in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica
2.1.1.258 10800
-
 5-methyltetrahydrofolate using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C Moorella thermoacetica