EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.47 | - |
Saccharolobus solfataricus |
1.1.1.47 | expression in Escherichia coli | Saccharolobus solfataricus |
1.1.1.359 | - |
Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.47 | NAD+ | above 12 mM | Saccharolobus solfataricus | |
1.1.1.47 | NADP+ | above 12 mM | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 0.17 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.47 | 4.59 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 0.18 | - |
D-xylose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.359 | 0.25 | - |
D-xylose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 0.44 | - |
D-galactose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.359 | 0.5 | - |
L-arabinose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 0.57 | - |
D-galactose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 1.3 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.359 | 1.5 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.47 | beta-D-glucose + NAD(P)(+) | Saccharolobus solfataricus | the enzyme might represent the major player in glucose catabolism via the branched Entner-Doudoroff pathway | D-glucono-1,5-lactone + NAD(P)H | - |
ir | |
1.1.1.47 | beta-D-glucose + NADP+ | Saccharolobus solfataricus | GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of NADP+ is about 8fold higher compared to kcat/Km of NAD+ | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
1.1.1.359 | additional information | Saccharolobus solfataricus | the enzyme iss involved in aldose catabolism via the branched variant of the EntnerDoudoroff pathway | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.47 | Saccharolobus solfataricus | Q97U21 | - |
- |
1.1.1.359 | Saccharolobus solfataricus | Q7LYI9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.47 | - |
Saccharolobus solfataricus |
1.1.1.359 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.47 | beta-D-glucose + NAD(P)(+) | the enzyme might represent the major player in glucose catabolism via the branched Entner-Doudoroff pathway | Saccharolobus solfataricus | D-glucono-1,5-lactone + NAD(P)H | - |
ir | |
1.1.1.47 | beta-D-glucose + NAD+ | GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km for glucose in the reaction with NAD+ is about 8fold lower compared to kcat/Km of glucose in the reaction with NADP+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
1.1.1.47 | beta-D-glucose + NAD+ | the enzyme is absolutely specific for glucose. D-Galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol are not used as substrates. the catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADH | - |
ir | |
1.1.1.47 | beta-D-glucose + NADP+ | GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of NADP+ is about 8fold higher compared to kcat/Km of NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
1.1.1.47 | beta-D-glucose + NADP+ | GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of glucose in the reaction with NADP+ is about 8fold higher compared to kcat/Km of glucose in reaction with NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
1.1.1.47 | beta-D-glucose + NADP+ | the enzyme is absolutely specific for glucose. D-Galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol are not used as substrates. the catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ | Saccharolobus solfataricus | D-glucono-1,5-lactone + NADPH | - |
ir | |
1.1.1.359 | beta-D-glucose + NAD+ + H2O | - |
Saccharolobus solfataricus | D-gluconate + NADH + 2 H+ | - |
ir | |
1.1.1.359 | beta-D-glucose + NADP+ + H2O | - |
Saccharolobus solfataricus | D-gluconate + NADPH + 2 H+ | - |
ir | |
1.1.1.359 | D-galactose + NAD+ + H2O | - |
Saccharolobus solfataricus | D-galactonate + NADH + 2 H+ | - |
? | |
1.1.1.359 | D-galactose + NADP+ + H2O | - |
Saccharolobus solfataricus | D-galactonate + NADPH + 2 H+ | - |
? | |
1.1.1.359 | D-xylose + NAD+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
1.1.1.359 | D-xylose + NADP+ + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
1.1.1.359 | L-arabinose + NADP+ + H2O | no activity with NAD+ | Saccharolobus solfataricus | ? | - |
? | |
1.1.1.359 | additional information | the enzyme iss involved in aldose catabolism via the branched variant of the EntnerDoudoroff pathway | Saccharolobus solfataricus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.47 | gdh-2 | - |
Saccharolobus solfataricus |
1.1.1.47 | SSO3204 | - |
Saccharolobus solfataricus |
1.1.1.359 | gdh-1 | - |
Saccharolobus solfataricus |
1.1.1.359 | SSO3003 | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 70 | - |
assay at | Saccharolobus solfataricus |
1.1.1.359 | 70 | - |
assay at | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 19.34 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.47 | 68.23 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 37 | - |
D-galactose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.359 | 41 | - |
L-arabinose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 47 | - |
D-xylose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.359 | 47.7 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.359 | 61 | - |
D-galactose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 61 | - |
D-xylose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 74.9 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 6.5 | - |
assay at | Saccharolobus solfataricus |
1.1.1.359 | 6.5 | - |
assay at | Saccharolobus solfataricus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.47 | NAD+ | catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ | Saccharolobus solfataricus | |
1.1.1.47 | NAD+ | kcat/Km of glucose in reaction with NAD+ is about 8fold lower compared to kcat/Km of glucose in the reaction with NADP+ | Saccharolobus solfataricus | |
1.1.1.47 | NADP+ | catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ | Saccharolobus solfataricus | |
1.1.1.47 | NADP+ | kcat/Km of glucose in reaction with NADP+ is about 8fold higher compared to kcat/Km of glucose in the reaction with NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | NAD+ | - |
Saccharolobus solfataricus | |
1.1.1.359 | NADP+ | - |
Saccharolobus solfataricus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.47 | physiological function | the enzyme is involved in glucose catabolism via the branched EntnerDoudoroff pathway | Saccharolobus solfataricus |
1.1.1.359 | physiological function | in glucose catabolism via the branched EntnerDoudoroff pathway the enzyme might acquire important function at higher D-glucose concentrations and in the presence of NAD+. It seems to have additional functions in the catabolism of D-galactose via the same pathway as well as in degradation pathways of D-xylose and L-arabinose | Saccharolobus solfataricus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 14.86 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.47 | 115.2 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.359 | 36.7 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.359 | 49.9 | - |
beta-D-glucose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 82 | - |
L-arabinose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 85 | - |
D-galactose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus | |
1.1.1.359 | 108 | - |
D-galactose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 245 | - |
D-xylose | pH 6.5, 70°C, cosubstrate: NAD+ | Saccharolobus solfataricus | |
1.1.1.359 | 261 | - |
D-xylose | pH 6.5, 70°C, cosubstrate: NADP+ | Saccharolobus solfataricus |