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Literature summary extracted from

  • Haferkamp, P.; Kutschki, S.; Treichel, J.; Hemeda, H.; Sewczyk, K.; Hoffmann, D.; Zaparty, M.; Siebers, B.
    An additional glucose dehydrogenase from Sulfolobus solfataricus: fine-tuning of sugar degradation? (2011), Biochem. Soc. Trans., 39, 77-81.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.47
-
Saccharolobus solfataricus
1.1.1.47 expression in Escherichia coli Saccharolobus solfataricus
1.1.1.359
-
Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.47 NAD+ above 12 mM Saccharolobus solfataricus
1.1.1.47 NADP+ above 12 mM Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.17
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.47 4.59
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 0.18
-
D-xylose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 0.25
-
D-xylose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 0.44
-
D-galactose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 0.5
-
L-arabinose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 0.57
-
D-galactose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 1.3
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 1.5
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.47 beta-D-glucose + NAD(P)(+) Saccharolobus solfataricus the enzyme might represent the major player in glucose catabolism via the branched Entner-Doudoroff pathway D-glucono-1,5-lactone + NAD(P)H
-
ir
1.1.1.47 beta-D-glucose + NADP+ Saccharolobus solfataricus GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of NADP+ is about 8fold higher compared to kcat/Km of NAD+ D-glucono-1,5-lactone + NADPH + H+
-
?
1.1.1.359 additional information Saccharolobus solfataricus the enzyme iss involved in aldose catabolism via the branched variant of the Entner–Doudoroff pathway ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Saccharolobus solfataricus Q97U21
-
-
1.1.1.359 Saccharolobus solfataricus Q7LYI9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47
-
Saccharolobus solfataricus
1.1.1.359
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 beta-D-glucose + NAD(P)(+) the enzyme might represent the major player in glucose catabolism via the branched Entner-Doudoroff pathway Saccharolobus solfataricus D-glucono-1,5-lactone + NAD(P)H
-
ir
1.1.1.47 beta-D-glucose + NAD+ GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km for glucose in the reaction with NAD+ is about 8fold lower compared to kcat/Km of glucose in the reaction with NADP+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADH + H+
-
?
1.1.1.47 beta-D-glucose + NAD+ the enzyme is absolutely specific for glucose. D-Galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol are not used as substrates. the catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADH
-
ir
1.1.1.47 beta-D-glucose + NADP+ GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of NADP+ is about 8fold higher compared to kcat/Km of NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH + H+
-
?
1.1.1.47 beta-D-glucose + NADP+ GDH-2 is absolutely specific for D-glucose. D-galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol were not used as substrates. kcat/Km of glucose in the reaction with NADP+ is about 8fold higher compared to kcat/Km of glucose in reaction with NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH + H+
-
?
1.1.1.47 beta-D-glucose + NADP+ the enzyme is absolutely specific for glucose. D-Galactose, D-allose, D-mannose, D-xylose, L-arabinose, D-ribose, D-xylose, D-arabinose, L-xylose, D-glucosamine, 2-deoxy-D-glucose, D-fucose, D-lactose, D-maltose, D-fructose and ethanol are not used as substrates. the catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ Saccharolobus solfataricus D-glucono-1,5-lactone + NADPH
-
ir
1.1.1.359 beta-D-glucose + NAD+ + H2O
-
Saccharolobus solfataricus D-gluconate + NADH + 2 H+
-
ir
1.1.1.359 beta-D-glucose + NADP+ + H2O
-
Saccharolobus solfataricus D-gluconate + NADPH + 2 H+
-
ir
1.1.1.359 D-galactose + NAD+ + H2O
-
Saccharolobus solfataricus D-galactonate + NADH + 2 H+
-
?
1.1.1.359 D-galactose + NADP+ + H2O
-
Saccharolobus solfataricus D-galactonate + NADPH + 2 H+
-
?
1.1.1.359 D-xylose + NAD+ + H2O
-
Saccharolobus solfataricus ?
-
?
1.1.1.359 D-xylose + NADP+ + H2O
-
Saccharolobus solfataricus ?
-
?
1.1.1.359 L-arabinose + NADP+ + H2O no activity with NAD+ Saccharolobus solfataricus ?
-
?
1.1.1.359 additional information the enzyme iss involved in aldose catabolism via the branched variant of the Entner–Doudoroff pathway Saccharolobus solfataricus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.47 gdh-2
-
Saccharolobus solfataricus
1.1.1.47 SSO3204
-
Saccharolobus solfataricus
1.1.1.359 gdh-1
-
Saccharolobus solfataricus
1.1.1.359 SSO3003
-
Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.47 70
-
assay at Saccharolobus solfataricus
1.1.1.359 70
-
assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.47 19.34
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.47 68.23
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 37
-
D-galactose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 41
-
L-arabinose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 47
-
D-xylose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 47.7
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 61
-
D-galactose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 61
-
D-xylose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 74.9
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 6.5
-
assay at Saccharolobus solfataricus
1.1.1.359 6.5
-
assay at Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NAD+ catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ Saccharolobus solfataricus
1.1.1.47 NAD+ kcat/Km of glucose in reaction with NAD+ is about 8fold lower compared to kcat/Km of glucose in the reaction with NADP+ Saccharolobus solfataricus
1.1.1.47 NADP+ catalytic efficiency for the reaction with beta-D-glucose and NADP+ (kcat/Km) is 7.8 fold higher compared to catalytic efficiency for the reaction with beta-D-glucose and NAD+ Saccharolobus solfataricus
1.1.1.47 NADP+ kcat/Km of glucose in reaction with NADP+ is about 8fold higher compared to kcat/Km of glucose in the reaction with NAD+ Saccharolobus solfataricus
1.1.1.359 NAD+
-
Saccharolobus solfataricus
1.1.1.359 NADP+
-
Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
1.1.1.47 physiological function the enzyme is involved in glucose catabolism via the branched Entner–Doudoroff pathway Saccharolobus solfataricus
1.1.1.359 physiological function in glucose catabolism via the branched Entner–Doudoroff pathway the enzyme might acquire important function at higher D-glucose concentrations and in the presence of NAD+. It seems to have additional functions in the catabolism of D-galactose via the same pathway as well as in degradation pathways of D-xylose and L-arabinose Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.47 14.86
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.47 115.2
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 36.7
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 49.9
-
beta-D-glucose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 82
-
L-arabinose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 85
-
D-galactose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus
1.1.1.359 108
-
D-galactose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 245
-
D-xylose pH 6.5, 70°C, cosubstrate: NAD+ Saccharolobus solfataricus
1.1.1.359 261
-
D-xylose pH 6.5, 70°C, cosubstrate: NADP+ Saccharolobus solfataricus